Bacillus urumqiensis sp. nov., a moderately haloalkaliphilic bacterium isolated from a salt lake

Abstract

A Gram-stain-positive, rod-shaped, aerobic and moderately haloalkaliphilic bacterium, designated BZ-SZ-XJ18 T , was isolated from the mixed water and sediment of a saline-alkaline lake located in the Xinjiang Uyghur Autonomous Region of China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BZ-SZ-XJ18T was a member of the genus Bacillus. , equivalent to 6.3 %, w/v, NaCl), at pH 6.5-10.0 (optimum pH 8.5-9.5) and at 8-41 ºC (optimum 37 ºC). The major cellular fatty acids were anteiso-C 15:0 (43.0 %), C 16:0 (18.1 %), iso-C 15:0 (11.3 %), anteiso-C 17:0 (8.0 %) and iso-C 16:0 (7.0 %). The major polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol. The main respiratory quinone was menaquinone-7 (MK-7), and the peptidoglycan type of the cell wall was A1g based on meso-diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 42.3 mol% (HPLC) or 41.4 mol% (T m ). On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain BZ-SZ-XJ18T is proposed to represent a novel species, Bacillus urumqiensis within the genus Bacillus. The type strain is BZ-SZ-XJ18 T (=DSM 29145 T =JCM 30195 T ).

Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it only to the members of the Subtilis and Cereus clades of species

“…Type strain: KCTC 33100=LMG 27257=JC167. The description of this species is the same as provided by Zhang et al [83] for Bacillus urumqiensis.…”

Polyhydroxybutyrate production by an extremely halotolerant Halomonas elongata strain isolated from the hypersaline meromictic Fără Fund Lake (Transylvanian Basin, Romania)

“…; Zhang et al . ; Porras et al . ), this is the first report on the isolation of a halotolerant PHB‐producing bacterium from inland hypersaline meromictic lakes.…”

Tandem mass tag‐based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae BZ‐SZ‐XJ29T, a halophilic bacterium with a broad salinity range for optimal growth

“…Na + /K + transport systems might not function in long-term osmoadaptation of A. halolimnae For most halophilic microorganisms, a high internal ion concentration is an essential strategy for coping with external salt concentrations (Gunde-Cimerman et al, 2018;Oren, 2013). K + influx systems or Na + efflux systems can help cells maintain Na + /K + homeostasis and generate ion gradients necessary for energy production to resist high osmotic pressure (Peng et al, 2020;Yaakop et al, 2016;Zhang, Li, Yan, et al, 2016;. Peng et al (2020) found that genes encoding Na + /K + transporters were up-regulated in Halomonas socia NY-011NY-011 when subjected to high salt stress at various time intervals (Peng et al, 2020).…”