Bacopaside N1 biosynthetic potential of endophytic Aspergillus sp. BmF 16 isolated from Bacopa monnieri

Abstract

Chemically unique environment of endophytes makes them to have various adaptive mechanisms for survival. One of such mechanisms involves the production of pharmacologically significant plant-specific metabolites. In the present study, 26 endophytic fungi were isolated from stem of Bacopa monnieri (L.) Wettst. plants. All the isolates were screened for bacopaside production property by HPLC. Among these, the fungal isolate BmF 16 which was identified as Aspergillus sp. was confirmed for bacopaside N1 production (m/z 796) by LC-MS/MS analysis. As the extract of BMF16 used in the study was prepared from the fifth generation of culture, the obtained result can be confirmed as due to fungal production of bacopaside. In addition, this property was identified only for one among the 26 fungi screened. As bacopaside N1 production in fungi has not yet been reported, the results of the study are novel.

Isolation and characterization of endophytic fungi having plant growth promotion traits that biosynthesizes bacosides and withanolides under in vitro conditions

“…Saponins [ 34 ] and terpenoids [ 35 ] have multiple therapeutic values and are found usually in medicinal and aromatic plants. It was found that three isolated endophytic fungi were able to produce saponins and terpenoids which are in similar lines to other reports [ 34 , 36 ]. The crude extract of Aspergillus terreus (strain SUBL206) showed the presence of phenolics, tannin, flavonoids, and steroids.…”

“…Among aforesaid strains, SUBL33 produce highest quantity of Bacoside A3 (4093 μg mL −1 ), Jujubogenin isomer of Bacopasaponin C (65,339 μg mL −1 ), and Bacopasaponin C (1325 μg mL −1 ) while Bacopasaponin II was produced maximum by SUBL51 (13,030 μg mL −1 ) (Table 7 ). Although, Jasim et al [ 36 ] have characterized and reported the synthesis of bacoside from Aspergillus spp. under in vitro conditions but it is a novelty of our work which reports both withanolides and bacosides from endophytes including A. terreus in same metabolites in reasonable quantity.…”

“…Although, Jasim et al [36] have characterized and reported the synthesis of bacoside from Aspergillus spp. under in vitro conditions but it is a novelty of our work which reports both withanolides and bacosides from endophytes including A. terreus in same metabolites in reasonable quantity.…”

Bacopa monnieri Extract As a Neuroprotective and Cognitive Enhancement Agent

“…For instance, in dementia and amnesia, the protein acetylcholinesterase (AChE) is implicated, while α -synuclein is associated with Parkinson's disease. Furthermore, β -amyloid is closely linked to Alzheimer's disease, and VGluT1, VGluT2, and VGluT3 are involved in schizophrenia [12,13]. The glutamate-8 receptor (mGluR8) plays a role in epileptic seizures.…”

Bioprospecting of endophytes in medicinal plants of Thar Desert: An attractive resource for biopharmaceuticals

“… - Cytotoxic and antimicrobial activity Jain et al [ 143 ]; Singh et al [ 148 ]; Katoch et al [ 169 ] - - Aspergillus sp. Bacopaside Jasim et al [ 170 ] Ephedra foliata Boiss. ex C.A.…”