Bacteremia caused by nonhemolytic group B streptococci

Cueto, Marina de; Sanchez, M.; Serrano, José Manuel Flores; Aguilar, José Martínez; Martínez, Rosa Alba Sola; Rosa, Manuel de la

doi:10.1016/s0196-4399(96)80013-4

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…Although it occurs very infrequently, nonhemolytic, nonpigmented GBS have been implicated in cases of neonatal disease (18). Although these strains grow perfectly in GM (4,15), methods that do not rely on either hemolysis or pigment production must be used to detect them.…”

Section: Discussionmentioning

confidence: 99%

Use of Granada Medium To Detect Group B Streptococcal Colonization in Pregnant Women

et al. 1999

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Direct inoculation onto Granada medium (GM) in plates and tubes was compared to inoculation into a selective Todd-Hewitt broth (with 8 μg of gentamicin per ml and 15 μg of nalidixic acid per ml) for detection of group B streptococci (GBS) in pregnant women with 800 vaginal and 450 vaginoanorectal samples. Comparatively, GM was found to be as sensitive as the selective broth for the detection of GBS in vaginal specimens and more sensitive than selective broth for the detection of GBS in vaginoanorectal samples (96 versus 82%). The use of GM improved the time to reporting of a GBS-positive result by at least 24 h and reduced the direct cost of screening. We have also found that the inconvenience of anaerobic incubation of GM plates can be avoided when a cover slide is placed upon the inoculum, because aerobic incubation in GM plates with cover slides causes GBS to develop the same pigmentation that it develops with incubation under anaerobic conditions. These data support the routine use of GM plates or tubes as a more accurate, easier, and cheaper method of identification of GBS-colonized women compared to the enrichment broth technique.

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Section: Discussionmentioning

confidence: 99%

Use of Granada Medium To Detect Group B Streptococcal Colonization in Pregnant Women

et al. 1999

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“…Nonhemolytic GBS strains isolated in clinical microbiology laboratories make up 1 to 2% of all strains of this microorganism (3) and are considered less virulent than hemolytic strains (9). Although the former strains have been implicated in cases of neonatal infection (5), the number of infections in adults caused by these microorganisms is reportedly extremely small (2). To the best of our knowledge, the following is the first reported case of endocarditis caused by a nonhemolytic GBS.…”

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Endocarditis caused by nonhemolytic group B streptococcus

et al. 1997

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We report a case of bacterial endocarditis caused by nonhemolytic group B streptococcus (GBS) in a 67-year-old man with no predisposing risk factors. Nonhemolytic GBS strains rarely cause illness and are usually detected in perinatal infections. We believe this to be the first reported case of endocarditis caused by a nonhemolytic strain of GBS.Cases of bacterial endocarditis caused by Streptococcus agalactiae (Lancefield group B streptococcus [GBS]) are rare, with fewer than 100 episodes reported in the literature in English (1,4,7,8). More than half of all cases arise in patients with risk factors such as cardiac disease, diabetes, alcoholism, solid or hematological tumors, peripheral vascular disease, and nephropathy. Nonhemolytic GBS strains isolated in clinical microbiology laboratories make up 1 to 2% of all strains of this microorganism (3) and are considered less virulent than hemolytic strains (9). Although the former strains have been implicated in cases of neonatal infection (5), the number of infections in adults caused by these microorganisms is reportedly extremely small (2). To the best of our knowledge, the following is the first reported case of endocarditis caused by a nonhemolytic GBS.A 67-year-old man came to the emergency service of a district hospital and was admitted for a systolic II/IV heart murmur with an aortic focus. The only antecedents of note were fever (more than 39°C) of 1 month's duration and a poor general condition. There were no antecedents of cardiovascular disease, hypertension, diabetes, or recent urinary or dental procedures. On day 2, blood cultures (BacTAlert system; Organon Teknika, Durham, N.C.) and urine cultures (cystine lactose electrolyte-deficient agar [CLED] medium) grew a nonhemolytic gram-positive coccus with streptococcal morphology, and imipenem and amikacin administration was started. On day 3, the patient developed severe aortic insufficiency and was transferred to our hospital for emergency valvular replacement. On admission, echocardiographic exploration revealed rupture of the papillary muscle, and a 2-by 2-cm vegetation on the aortic valve was detected. Three sets of aerobic and anaerobic FAN blood culture bottles (BactTAlert system) were then inoculated.After valvular replacement, Gram staining of material from the valve showed abundant gram-positive cocci resembling streptococci, and antibiotic therapy was switched to vancomycin (500 mg/6 h given intravenously [i.v.]) plus amikacin (7.5 mg/kg of body weight/12 h given i.v.) until identification and susceptibility studies of the microorganism were available.Slices of the native valve were homogenized in brain heart infusion broth and cultured in blood agar (Columbia agar base plus 5% sheep blood) aerobically and under an anaerobic atmosphere (7% CO 2 , 10% H 2 , 83% N 2 ) and in chocolate agar (5% CO 2 atmosphere). A pure culture of grey, convex, creamy, nonhemolytic colonies developed in all culture media after 18 h of incubation at 37°C. Colonies were oxidase and catalase negative, and Gram staining ...

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Bacteremia caused by nonhemolytic group B streptococci

Cited by 2 publications

References 8 publications

Use of Granada Medium To Detect Group B Streptococcal Colonization in Pregnant Women

Use of Granada Medium To Detect Group B Streptococcal Colonization in Pregnant Women

Endocarditis caused by nonhemolytic group B streptococcus

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