Use of Granada Medium To Detect Group B Streptococcal Colonization in Pregnant Women

Rosa-Fraile, Manuel; Rodríguez‐Grangér, Javier; Cueto-López, Marina; Sampedro, Antonio; Gaye, Enrique Biel; Haro, Juan; Andreu, Antònia

doi:10.1128/jcm.37.8.2674-2677.1999

Cited by 66 publications

(20 citation statements)

References 23 publications

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“…However, variable results have been reported using different media preparations while studying direct plating versus subculture from Lim broth. Some studies found comparable sensitivity of direct plating on a differential (Granada) medium compared with Lim broth enrichment (Gil et al , 1999; Votava et al , 2001), whereas other reports (De la Rosa et al , 1992; Rosa-Fraile et al , 1999) suggested that direct plating on chromogenic and or selective media was significantly less sensitive (Overman et al , 2002; Nomura et al , 2006). Comparing direct plating on SBS or CNA (bioMérieux) to that of plating on the same media after Lim broth enrichment was found to be equally sensitive with no significant advantage for enrichment (Tazi et al , 2009; El Aila et al , 2010; Louie et al , 2010).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of chromogenic medium and direct latex agglutination test for detection of group B streptococcus in vaginal specimens from pregnant women in Lebanon and Kuwait

Ghaddar

Anastasiadis

Jiser

et al. 2014

Journal of Medical Microbiology

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This study was undertaken to evaluate chromogenic medium and a direct latex agglutination test (DLA) for detection of Group B Streptococcus (GBS) in the vaginal specimens of pregnant women, and to ascertain the prevalence of GBS in this population in Kuwait and Lebanon. Vaginal swabs, collected from women at 35-37 weeks of gestation, were cultured on 5 % sheep blood agar (SBA), colistin nalidixic acid agar (CNA), Strept B Select chromogenic agar (SBS) as well as Lim enrichment broth in 168 cases in Lebanon while only SBA was used for 1391 samples in Kuwait. In addition, vaginal samples from 102 GBS-positive and 20 GBS-negative women near the time of delivery were collected in Kuwait for evaluation of the DLA test. During the study period, the prevalence of GBS colonization was determined to be 20.7 % (288/1391) in Kuwait while 18.4 % (31) of 168 pregnant women in Lebanon had vaginal cultures positive for GBS. By direct plating of vaginal swabs on the three media used, the isolation rates of GBS were 51.6, 64.5 and 77.4 % on SBA, CNA and SBS, respectively, which increased to 90.35, 93.1 and 96.8 %, respectively, following subculture in Lim broth after 18 h of incubation. The sensitivity of the DLA test was found to be dependent on the density of GBS colonization, resulting in 100 % sensitivity and 100 % specificity for heavy (.10 2 c.f.u. per swab) and moderately heavy (50-100 c.f.u. per swab) growth of GBS. However, for vaginal specimens yielding ,50 c.f.u. per swab, the sensitivity, specificity, positive and negative predictive values of the DLA test were 100, 55.5, 63.6 and 100 %, respectively. In conclusion, a chromogenic agar, such as SBS, and a DLA test can be used for rapid detection of GBS in pregnant women. The DLA test, in particular, could prove to be a useful tool for immediate detection of GBS in women near delivery so that intrapartum antibiotic prophylaxis can be initiated.

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Section: Discussionmentioning

confidence: 99%

Evaluation of chromogenic medium and direct latex agglutination test for detection of group B streptococcus in vaginal specimens from pregnant women in Lebanon and Kuwait

Ghaddar

Anastasiadis

Jiser

et al. 2014

Journal of Medical Microbiology

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“…Limitations of Granada medium are the requirement for anaerobiasis and its limited ability at suppressing the swarming growth of Proteus sp., which was recovered from four samples. The requirement for anaerobic incubation can be circumvented by the application of a cover slide on the inoculum, as described by Rosa‐Fraile et al. (1999).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a new chromogenic agar medium for isolation and identification of Group B streptococci

Perry

Oliver

Nicholson

et al. 2006

Lett Appl Microbiol

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Aims: To evaluate a new chromogenic agar as a screening medium for the isolation of Group B streptococci from high vaginal swabs from pregnant women. Methods and Results: The medium was evaluated with 195 high vaginal swabs referred for antenatal screening and compared with blood agar and Granada medium. The new chromogenic medium showed 100% sensitivity for the detection of Group B streptococci, and also showed a positive predictive value of 100%. Granada medium also showed excellent sensitivity and specificity and both media were superior to blood agar. Conclusions: The new chromogenic medium showed excellent performance for the detection of Group B streptococci from clinical samples. Significance and Impact of the Study: This is the first chromogenic medium described for the detection of Group B streptococci. The medium offers an effective and convenient alternative to conventional media, currently used in clinical laboratories.

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“…Fallon (1974) proposed the use of pigment detection as a diagnostic tool for GBS identification. This orange, brick or red pigment is unique and highly specific for GBS isolates and is used in the clinical laboratory for the identification of GBS (Fallon, 1974;Noble et al, 1983;Rosa-Fraile et al, 1999b;Spellerberg & Brandt, 2011). Nevertheless, pigment production can be variable among bovine strains and other animal species isolates (Mhalu, 1976;Merritt & Jacobs, 1978;Brglez, 1983;L€ ammler et al, 1985;Garcia et al, 2008).…”

Section: Biological Characteristicsmentioning

confidence: 99%

Group B streptococcal haemolysin and pigment, a tale of twins

Rosa-Fraile¹,

Dramsi²,

Spellerberg³

2014

FEMS Microbiol Rev

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Group B streptococcus [(GBS or Streptococcus agalactiae)] is a leading cause of neonatal meningitis and septicaemia. Most clinical isolates express simultaneously a β-haemolysin/cytolysin and a red polyenic pigment, two phenotypic traits important for GBS identification in medical microbiology. The genetic determinants encoding the GBS haemolysin and pigment have been elucidated and the molecular structure of the pigment has been determined. The cyl operon involved in haemolysin and pigment production is regulated by the major two-component system CovS/R, which coordinates the expression of multiple virulence factors of GBS. Genetic analyses indicated strongly that the haemolysin activity was due to a cytolytic toxin encoded by cylE. However, the biochemical nature of the GBS haemolysin has remained elusive for almost a century because of its instability during purification procedures. Recently, it has been suggested that the haemolytic and cytolytic activity of GBS is due to the ornithine rhamnopolyenic pigment and not to the CylE protein. Here we review and summarize our current knowledge of the genetics, regulation and biochemistry of these twin GBS phenotypic traits, including their functions as GBS virulence factors.

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Use of Granada Medium To Detect Group B Streptococcal Colonization in Pregnant Women

Cited by 66 publications

References 23 publications

Evaluation of chromogenic medium and direct latex agglutination test for detection of group B streptococcus in vaginal specimens from pregnant women in Lebanon and Kuwait

Evaluation of chromogenic medium and direct latex agglutination test for detection of group B streptococcus in vaginal specimens from pregnant women in Lebanon and Kuwait

Evaluation of a new chromogenic agar medium for isolation and identification of Group B streptococci

Group B streptococcal haemolysin and pigment, a tale of twins

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