Bacteria‐Based Materials for Stem Cell Engineering

Hay, Jake J.; Rodrigo‐Navarro, Aleixandre; Petaroudi, Michaela; Bryksin, Anton V.; Garcı́a, Andrés J.; Barker, Thomas H.; Dalby, Matthew J.; Salmerón-Sánchez, Manuel

doi:10.1002/adma.201804310

Cited by 58 publications

(57 citation statements)

References 46 publications

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“…[132] Hay et al have recently introduced an innovative approach to present these binding motifs on material surfaces. [133] Lactococcus lactis bacteria able to spontaneously colonize a variety of material surfaces were engineered to become polypeptide-presenting units in order to control human MSCs in a highly dynamic temporal manner. L. lactis were engineered to express a membrane-bound FN III 7-10 (integrin-binding properties) creating a eukaryotic cell-biomaterial interface which allowed good cell adhesion and spreading.…”

Section: Platforms With Inclusion Of Ecm Componentsmentioning

confidence: 99%

“…This allowed a temporal control over the production of the GF, and therefore triggered osteogenic differentiation in an ondemand manner. [133] FN is indubitably a versatile and effective way to sequester and present GFs for tailored control of stem cell responses. Nonetheless, like heparin, the affinity-binding properties of its III 12-14 domain are promiscuous, which does not allow for the capture of a specific endogenous GF.…”

Section: Platforms With Inclusion Of Ecm Componentsmentioning

confidence: 99%

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Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

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Growth factors (GFs) are proteins secreted by cells that regulate a variety of biological processes. Although they have long been proposed as potent therapeutic agents, their administration in a soluble form has proven costly and ineffective due to their short half-lives in biological environments. Biomaterial-based approaches are increasingly sought as alternatives to improve the efficacy or, ideally, replace the need for exogenous administration of GFs in regenerative medicine strategies. The means by which these systems evolve from biomaterials for conventional controlled release of GFs to the recent extracellular matrix (ECM)-inspired approaches for sequestering these labile molecules and regulating their spatiotemporal activity and presentation are reviewed. Focus is placed on biomaterials functionalized either with ECM components, which show promiscuous GF binding, or with targeted GF ligands (antibodies, aptamers, or peptides). The potential of synthetic platforms with abiotic affinity as cost-effective alternatives to the current biological ligands is also discussed. Overall, the various GF sequestering systems developed so far have remarkably improved the activity of GFs at reduced doses and, in some cases, completely avoided the need for their exogenous administration to guide cell fates. These bioinspired concepts thus enable the rational exploration of the full therapeutic potential of GFs in regenerative medicine.

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Section: Platforms With Inclusion Of Ecm Componentsmentioning

confidence: 99%

Section: Platforms With Inclusion Of Ecm Componentsmentioning

confidence: 99%

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

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show abstract

“…11 A similar materials approach has also been reported using bacteria to form a living interface between material surfaces and mammalian cells either for intracellular delivery of proteins 12 or extracellular delivery of growth factors inducing stem-cell differentiation. 13 It should be noted that bacteria are robust and have been successfully entrapped in a viable and functional manner within various hydrogel matrices. Alginate, 14 polyvinyl alcohol, 15 polyacrylamide (PAAM), 16 Pluronic F127, 17 etc.…”

Section: Introductionmentioning

confidence: 99%

Optoregulated Drug Release from an Engineered Living Hydrogel

Sankaran¹,

Becker²,

Wittmann³

et al. 2018

Preprint

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“…Stiffness of materials that are used in stem cell culturing is shown as a critical parameter in cell differentiation and self‐renewal . Living base materials that evolve their material properties based on the cell status can be engineered for organoid formation and stem cell engineering . In addition, patterning of E. coli cells on textile materials such as cotton and plastics through functionalized CsgA variants makes it possible to develop responsive clothes that can sense small molecules or replace the body odor with more pleasant scents .…”

mentioning

confidence: 99%

Genetic Logic Gates Enable Patterning of Amyloid Nanofibers

et al. 2019

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Distinct spatial patterning of naturally produced materials is observed in many cellular structures and even among communities of microorganisms. Reoccurrence of spatially organized materials in all branches of life is clear proof that organization is beneficial for survival. Indeed, organisms can trick the evolutionary process by using organized materials in ways that can help the organism to avoid unexpected conditions. To expand the toolbox for synthesizing patterned living materials, Boolean type “AND” and “OR” control of curli fibers expression is demonstrated using recombinases. Logic gates are designed to activate the production of curli fibers. The gates can be used to record the presence of input molecules and give output as CsgA expression. Two different curli fibers (CsgA and CsgA‐His‐tag) production are then selectively activated to explore distribution of monomers upon coexpression. To keep track of the composition of fibers, CsgA‐His‐tag proteins are labeled with nickel–nitrilotriacetic acid (Ni–NTA‐) conjugated gold nanoparticles. It is observed that an organized living material can be obtained upon inducing the coexpression of different CsgA fibers. It is foreseen that living materials with user‐defined curli composition hold great potential for the development of living materials for many biomedical applications.

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Bacteria‐Based Materials for Stem Cell Engineering

Cited by 58 publications

References 46 publications

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Optoregulated Drug Release from an Engineered Living Hydrogel

Genetic Logic Gates Enable Patterning of Amyloid Nanofibers

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