Bacteria from Arbuscular Mycorrhizal Fungi Spores Gigaspora sp. and Glomus sp. : Their Antagonistic Effects towards Soilborne Fungal Pathogens and Growth Stimulation of Gigaspora sp. in vitro

Abstract

This research was aimed at obtaining bacterial isolate from arbuscular mycorrhizal fungi (AMF) spores showing antagonistic effects against fungal pathogens and yet compatible with AMF. Seven isolates of bacteria were isolated from surface-sterilized AMF spores of sp. (GG) and five isolates of bacteria isolated from sp. (GL). All bacterial isolates were identified based on its morphological methods and biochemical reaction and revealed that 9 isolates belong to genus , while the other 3 isolates belong to genus , and respectively. The tests to the antagonists against fungal pathogens and stimulation of AMF hyphal development of sp. showed that there were 3 isolates of bacteria (GG1, GG5 and GL3) had the ability to inhibit the growth of pathogens and to enhance the development of AMF hyphae sp. These three bacteria isolates potentially can be used to enhance the quality of AMF inoculants as biocontrol and biofertilizers. Enzymatic activity test showed that there were 7 isolates of bacteria that produced cellulase and protease activities, i.e. GG1,