Bacterial Adhesion and Biomaterial Surfaces

Moriarty, T. Fintan; Poulsson, Alexandra H.C.; Rochford, Edward T.J.; Richards, R. Geoff

doi:10.1016/b978-0-08-055294-1.00007-6

Cited by 25 publications

(37 citation statements)

References 174 publications

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“…34,39 Agar hydrogels adsorbed significant Fibronectin, exceeding glass controls, which adsorbed significantly more protein than the PEGDMA. Since most mechanisms of bacterial adhesion are protein-mediated, 53,54 the presence of protein on the agar hydrogel surface serves as a positive control for the PEGDMA hydrogels that resist protein adsorption over the timescale of this study, 24 hr.…”

Section: Resultsmentioning

confidence: 99%

Fewer Bacteria Adhere to Softer Hydrogels

Kolewe

Peyton

Schiffman

2015

ACS Appl. Mater. Interfaces

114

140

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Clinically, biofilm-associated infections commonly form on intravascular catheters and other hydrogel surfaces. The overuse of antibiotics to treat these infections has led to the spread of antibiotic resistance and underscores the importance of developing alternative strategies that delay the onset of biofilm formation. Previously, it has been reported that during surface contact, bacteria can detect surfaces through subtle changes in the function of their motors. However, how the stiffness of a polymer hydrogel influences the initial attachment of bacteria is unknown. Systematically, we investigated poly(ethylene glycol) dimethacrylate (PEGDMA) and agar hydrogels that were twenty times thicker than the cumulative size of bacterial cell appendages, as a function of Young’s moduli. Soft (44.05 – 308.5 kPa), intermediate (1495 – 2877 kPa), and stiff (5152 – 6489 kPa) hydrogels were synthesized. Escherichia coli and Staphylococcus aureus attachment onto the hydrogels was analyzed using confocal microscopy after 2 and 24 hr incubation periods. Independent of hydrogel chemistry and incubation time, E. coli and S. aureus attachment correlated positively to increasing hydrogel stiffness. For example, after a 24 hr incubation period, there were 52% and 82% less E. coli adhered to soft PEGDMA hydrogels, than to the intermediate and stiff PEGDMA hydrogels, respectively. A 62% and 79% reduction in the area coverage by the Gram-positive microbe S. aureus occurred after 24 hr incubation on the soft versus intermediate and stiff PEGDMA hydrogels. We suggest that hydrogel stiffness is an easily tunable variable that, potentially, could be used synergistically with traditional antimicrobial strategies to reduce early bacterial adhesion, and therefore the occurrence of biofilm-associated infections.

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Section: Resultsmentioning

confidence: 99%

Fewer Bacteria Adhere to Softer Hydrogels

Kolewe

Peyton

Schiffman

2015

ACS Appl. Mater. Interfaces

114

140

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“…However, in recent years, other materials such as PEEK have also been used (Santing, Meijer, Raghoebar, & Özcan, 2012;Stawarczyk et al, 2013). Even though PEEK has many advantages, as with all biomaterials, undesired tissue reactions (e.g., allergic reactions) and infections may occur (Moriarty, Poulsson, Rochford, & Richards, 2011).…”

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confidence: 99%

Biofilm formation on polyetheretherketone and titanium surfaces

Barkarmo

Longhorn

Leer

et al. 2019

Clinical & Exp Dental Res

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Objective Polyetheretherketone (PEEK) is a polymer used in devices in orthopedic and dental rehabilitation. The aim of this in vitro study was to compare biofilm formation by a range of important oral bacterial species on PEEK, blasted PEEK, commercially pure titanium (cp‐Ti), and titanium‐6 aluminium‐4 vanadium (Ti6Al4V). Material and methods Coin‐shaped samples were manufactured, and the surfaces were characterized using optical interferometry, scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, and contact angle measurements. Bacterial species of Streptococcus sanguinis, Streptococcus oralis, Enterococcus faecalis , and Streptococcus gordonii were cultured on the four material surfaces for varying amounts of time. Biofilms were quantified following staining with crystal violet. Results Roughness and contact angle results showed blasted PEEK > PEEK > cp‐Ti = Ti6Al4V. There was increased biofilm formation on blasted PEEK by S. sanguinis, S. oralis , and S. gordonii , whereas the bacterial adhesion was similar on PEEK, cp‐Ti, and Ti6Al4V. The bacterial growth of E. faecalis was significantly higher on cp‐Ti compared with the other three groups. Conclusion The results, taking into consideration the biofilm formation, suggest that PEEK should perform as well as cp‐Ti or TiAl6V4 when used as a dental restorative material.

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“…It is believed that the adhesion of bacteria to the surface is a complicated physical-chemical process, which depends on: the properties of a surface (topography, roughness, hydrophobicity, chemical composition, surface energy) [13,14]. It also depends on the initial number of microorganisms, their shape, size, temperature, and pH of the environment, etc.…”

Section: Literature Review and Problem Statementmentioning

confidence: 99%

“…However, among many of those specified factors that affect the process of adhesion, researchers point to the role of the properties of a surface, which is considered to be the most essential [17,18]. Consequently, three theories of microbial adhesion to the surface were proposed: thermodynamic, DLVO theory, and XDLVO [13]. The thermodynamic theory is based on that when microorganisms attach to the surface there is a change in the total Gibbs free energy, van der Waals forces.…”

Section: Literature Review and Problem Statementmentioning

confidence: 99%

Modeling the process of microbial biofilm formation on stainless steel with a different surface roughness

Kukhtyn

Kravcheniuk

Beyko

et al. 2019

EEJET

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Наведено дослідження процесу адгезії бактерій до поверхні з різною шорсткістю залежно від розмірів і форми. Встановлено, що на поверхні нержавіючої сталі з шорсткістю 2,687±0,014 мкм, процес плівкоутворення у E. coli та S. aureus проходив однаково упродовж з 3 до 24 години та не залежав від розмірів бактерій. Це дозволяє стверджувати, що паличковидні і кокові бактерії вільно прикріпляються у западинах шорсткості та розпочинається початковий процес першої стадії формування біоплівки. Під час санітарної обробки у западинах шорсткості можуть залишатися, як кокові, так паличковидні бактерії. На поверхні сталі з шорсткістю 0,95±0,092 мкм процес плівкоутворення у S. aureus проходив інтенсивніше, ніж у E. coli. Упродовж 3 год інкубації щільність сформованих біоплівок S. aureus була в 1,2 раза більша, порівняно з біоплівками E. coli. У наступні 15 годин інкубації сформовані біоплівки S. aureus були, в середньому в 1,3 раза щільніші. Це дає підставу вважати, що S. aureus завдяки кулястій формі здатний розміщуватися у западинах шорсткості 0,95±0,092 мкм і швидше адгезуватися до поверхні. Водночас E. coli, завдяки паличковидній формі, за такої шорсткості поверхні може адгезуватися у западини тільки повздовж. Доведено, що за шорсткості поверхні 0,63±0,087 мкм інтенсивність плівкоутворення S.aureus була, в середньому в 1,4 раза швидша, ніж у E. coli. Водночас, за шорсткості 0,16±0,018 мкм процес плівкоутворення проходив однаково у S. aureus і E. coli, але біоплівки були нижчої щільності, порівняно з такими, які формувалися за шорсткості 0,63±0,087 мкм. Отже, використання обладнання у молочній промисловості з шорсткістю менше 0,5 мкм дозволить зменшення прикріплення мікроорганізмів до поверхні і зниження контамінації молочних продуктів Ключові слова: мікробна адгезія, формування біоплівок, шорсткість поверхні нержавіючої сталі, процес плівкоутворення

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Bacterial Adhesion and Biomaterial Surfaces

Cited by 25 publications

References 174 publications

Fewer Bacteria Adhere to Softer Hydrogels

Fewer Bacteria Adhere to Softer Hydrogels

Biofilm formation on polyetheretherketone and titanium surfaces

Modeling the process of microbial biofilm formation on stainless steel with a different surface roughness

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