Bacterial adhesion on PEG modified polyurethane surfaces

Парк, Ки Донг; Kim, Young Soo; Han, Dong Keun; Lee, Eun Hee Bae; Suh, Hwal; Choi, Kyu Suk

doi:10.1016/s0142-9612(97)00245-7

Cited by 414 publications

(262 citation statements)

References 22 publications

Supporting

Mentioning

249

Contrasting

Unclassified

Order By: Relevance

“…PEO-brushes were characterized by various surface-sensitive techniques. Water contact angles indicated that pristine glass and silica surfaces were more hydrophilic than the PEO-coated surfaces, as in other studies (Park et al, 1998;Lee & Laibinis, 1998). The high contact angle hysteresis (>25 u ) found for both PEO-coated surfaces suggests penetration of water in the PEO layer and mobility of the PEO chains (Andrade et al, 1985).…”

Section: Discussionsupporting

confidence: 70%

“…This is a larger reduction than usually achieved in vitro with changing the hydrophobicity or other surface modifications. Park et al (1998) showed that reduced Lifshitz-Van der Waals attraction by a PEO-coating with a molar mass of 1000 was not accompanied by a reduction in adhesion of Sta. epidermidis ATCC 12228.…”

Section: Discussionmentioning

confidence: 99%

“…Therewith, a PEO-brush forms a steric barrier preventing close approach and thus attenuates the attractive Lifshitz-Van der Waals interaction between the protein and the surface. PEO-coatings have also been investigated for their ability to prevent bacterial adhesion (Park et al, 1998;Razatos et al, 2000;Ista et al, 1996;Bridgett et al, 1992). Varying results have been obtained, ranging from reductions in bacterial adhesion of 98 % (Razatos et al, 2000;Ista et al 1996), 90-98 % (Bridgett et al, 1992) and between 0 and 90 % (Park et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…PEO-coatings have also been investigated for their ability to prevent bacterial adhesion (Park et al, 1998;Razatos et al, 2000;Ista et al, 1996;Bridgett et al, 1992). Varying results have been obtained, ranging from reductions in bacterial adhesion of 98 % (Razatos et al, 2000;Ista et al 1996), 90-98 % (Bridgett et al, 1992) and between 0 and 90 % (Park et al, 1998). These studies, however, have used only a small number of strains and the adhesion methodology employed involved dipping and slight rinsing, which may also cause detachment and accompanying low adhesion numbers (Gò mez-Suárez et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Inhibition of adhesion of yeasts and bacteria by poly(ethylene oxide)-brushes on glass in a parallel plate flow chamber

et al. 2003

View full text Add to dashboard Cite

Poly(ethylene oxide) (PEO)-brushes are generally recognized as protein-repellent surfaces, and although a role in discouraging microbial adhesion has been established for some strains and species, no study exists on the effects of PEO-brushes on a large variety of bacterial and yeast strains. In this paper, a PEO-brush has been covalently attached to glass and silica by reaction in a polymer melt. Subsequently, the presence of a PEO-brush was demonstrated using contact angle measurements, X-ray photoelectron spectroscopy and ellipsometry. For five bacterial (Staphylococcus epidermidis, Staphylococcus aureus, Streptococcus salivarius, Escherichia coli and Pseudomonas aeruginosa) and two yeast strains (Candida albicans and Candida tropicalis), adhesion to PEO-brushes was compared with adhesion to bare glass in a parallel plate flow chamber. The initial deposition rates of Sta. epidermidis, Sta. aureus and Str. salivarius to glass were relatively high, between 2400 and 2600 cm "2 s "1 , while E. coli and P. aeruginosa deposited much more slowly. The initial deposition rates of the yeasts to glass were 144 and 444 cm "2 s "1 for C. albicans GB 1/2 and C. tropicalis GB 9/9, respectively. Coating of the glass surface with a PEO-brush yielded more than 98 % reduction in bacterial adhesion, although for the more hydrophobic P. aeruginosa a smaller reduction was observed. For both yeast species adhesion suppression was less effective than for the bacteria and here too the more hydrophobic C. tropicalis showed less reduction than the more hydrophilic C. albicans. The PEO-brush had a thickness of 22 nm in water, as inferred from ellipsometry. Assuming that on bare glass the adhered micro-organisms are positioned only a few nanometers away from the surface and that the brush keeps them at a distance of 22 nm, it is calculated that the brush yields a sevenfold attenuation of the Lifshitz-Van der Waals attraction to the surface between the micro-organisms and the surface. Decreased Lifshitz-van der Waals attraction may be responsible for the suppression of the microbial adhesion observed.

show abstract

Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Inhibition of adhesion of yeasts and bacteria by poly(ethylene oxide)-brushes on glass in a parallel plate flow chamber

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Estudos têm demonstrado a comum contaminação de superfícies em hospitais, tais como maçanetas de portas (OIE et al, 2002), embalagens estéreis (DIETZE et al, 2001), tecidos e plásticos utilizados pela enfermagem (NEELY & MALEY, 2000), canetas utilizadas pelos funcionários (BANERJEE et al, 1999), teclados e torneiras (BURES et al, 2000), estetoscópios (COHEN et al, 1997) et al, 1975;DESAI et al, 1992;BRIDGETT et al, 1992;ARCIOLA et al, 1993;KOHNEN & JANSEN, 1995;PARK et al, 1998). Ainda existem as superfícies revestidas com polímeros zwiteriônicos.…”

Section: Filmes E Superfícies Com Propriedades Antimicrobianasunclassified

Partículas e filmes híbridos de polímeros e compostos de amônio quaternário com atividade antimicrobiana

Melo¹

View full text Add to dashboard Cite

Effects of surface-coupled polyethylene oxide on human macrophage adhesion and foreign body giant cell formationin vitro

Jenney

Anderson

1999

J. Biomed. Mater. Res.

107

View full text Add to dashboard Cite

Surface immobilized polyethylene oxide (PEO) has been shown to efficiently reduce protein adsorption and cellular adhesion, resulting in a biologically passive surface. To explore the in vitro effects of surface immobilized PEO on the human inflammatory cells, macrophages, and foreign body giant cells (FBGCs), we developed a diisocyanate-based method for coupling PEO to amine-modified glass, a surface previously shown to enhance macrophage adhesion and FBGC formation. Contact angle analysis and X-ray photoelectron spectroscopy confirmed the presence of PEO molecules bound to the surface and revealed that PEO molecular weight significantly influenced the efficiency of PEO coupling. We used a 10-day human monocyte culture protocol to demonstrate that the presence of surface coupled PEO molecules does not significantly decrease initial monocyte density or monocyte-derived macrophage density after 3 days. However, PEO-coupled surfaces significantly reduced long-term monocyte-derived macrophage density and virtually eliminated interleukin-4-induced FBGC formation observed at day 10. The cellular response to these PEO-coupled surfaces was related to the molecular weight of the PEO chains, which was varied between 200 Da and 18.5 kDa. These results suggest that an optimized PEO surface treatment may be effective in reducing inflammatory cell adhesion and possible degradation during the inflammatory response to an implanted biomedical device.

show abstract

Bacterial adhesion on PEG modified polyurethane surfaces

Cited by 414 publications

References 22 publications

Inhibition of adhesion of yeasts and bacteria by poly(ethylene oxide)-brushes on glass in a parallel plate flow chamber

Inhibition of adhesion of yeasts and bacteria by poly(ethylene oxide)-brushes on glass in a parallel plate flow chamber

Partículas e filmes híbridos de polímeros e compostos de amônio quaternário com atividade antimicrobiana

Effects of surface-coupled polyethylene oxide on human macrophage adhesion and foreign body giant cell formationin vitro

Contact Info

Product

Resources

About