Bacterial adhesion onto apatite minerals —electrokinetic aspects

Rao, Michaël; Somasundaran, P.; Schilling, K; Carson, Bonnie L.; Ananthapadmanabhan, K.P.

doi:10.1016/0927-7757(93)80182-e

Cited by 21 publications

(12 citation statements)

References 12 publications

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“…4). The morphology of these curves is similar to reported data for calcium phosphates (Ducheyne et al, 1992;Yelloji Rao et al, 1993;Borum & Wilson, 2003;Smith et al, 2004). Negative f-potential values obtained for these bioceramics, in the pH range tested, range between 6 and 18 mV for HA and between 11 and 21 mV for BCP.…”

Section: F-potential Determinationssupporting

confidence: 88%

Influence of surface porosity and pH on bacterial adherence to hydroxyapatite and biphasic calcium phosphate bioceramics

Kinnari

Esteban

Martín-de-Hijas

et al. 2009

Journal of Medical Microbiology

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Hydroxyapatite (HA) and biphasic calcium phosphate (BCP) ceramic materials are widely employed as bone substitutes due to their porous and osteoconductive structure. Their porosity and the lowering of surrounding pH as a result of surgical trauma may, however, predispose these materials to bacterial infections. For this reason, the influence of porosity and pH on the adherence of common Gram-positive bacteria to the surfaces of these materials requires investigation. Mercury intrusion porosimetry measurements revealed that the pore size distribution of both bioceramics had, on a logarithmic scale, a sinusoidal frequency distribution ranging from 50 to 300 nm, with a mean pore diameter of 200 nm. Moreover, total porosity was 20 % for HA and 50 % for BCP. Adherence of Staphylococcus aureus and Staphylococcus epidermidis was studied at a physiological pH of 7.4 and at a pH simulating bone infection of 6.8. Moreover, the effect of pH on the f potential of HA, BCP and of both staphylococci was evaluated. Results showed that when pH decreased from 7.4 to 6.8, the adherence of both staphylococci to HA and BCP surfaces decreased significantly, although at the same time the negative f-potential values of the ceramic surfaces and both bacteria diminished. At both pH values, the number of S. aureus adhered to the HA surface appeared to be lower than that for BCP. A decrease in pH to 6.8 reduced the adherence of both bacterial species (mean 57 %). This study provides evidence that HA and BCP ceramics do not have pores sufficiently large to allow the internalization of staphylococci. Their anti-adherent properties seemed to improve when pH value decreased, suggesting that HA and BCP bioceramics are not compromised upon orthopaedic use.

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Section: F-potential Determinationssupporting

confidence: 88%

Influence of surface porosity and pH on bacterial adherence to hydroxyapatite and biphasic calcium phosphate bioceramics

Kinnari

Esteban

Martín-de-Hijas

et al. 2009

Journal of Medical Microbiology

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“…This is confirmed by the electrophoretic mobility which becomes more negative after adsorption. Similar observations were made for succinylated lysozyme (4), bovin serum albumin (9), and for bacteria (10) with hydroxyapatite. Rosa et al (11) showed also that catalase adsorbs on carbon black under conditions of electrostatic repulsion.…”

Section: Adsorption Mechanismsupporting

confidence: 76%

Adsorption of Catalase on Hydroxyapatite

Barroug

Lernoux²,

Lemaître

et al. 1998

Journal of Colloid and Interface Science

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“…A study by van Merode et al [14] showed that the clinical Enterococcus faecalis strains with less negative zeta potential adhered significantly better on polystyrene. On the other hand Rao et al [25] suggested that electrostatic interactions are not the primary factors determining adhesion of Streptococcus sanguis and Actinomyces naeslundii to apatite minerals. Similar deduction was obtained in a study on immobilization of A. junii on surfactant-modified zeolites [12].…”

Section: Immobilization Dependent On the Zeta Potentialmentioning

confidence: 99%