Bacterial and fungal gut communities of Agrilus mali at different developmental stages and fed different diets

Zhang, Zhengqing; Jiao, Shuo; Li, Xiaohui; Meng-lou, LI

doi:10.1038/s41598-018-34127-x

Cited by 48 publications

(54 citation statements)

References 61 publications

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“…It was suggested that C. pomonella could obtain the nutrients necessary for their own growth and development by feeding different host plants in the complex environment. The high gut microbial diversity observed in shoot‐feeding G. molesta may have resulted from the vast microbiota via a specific nutritional complementation from an imbalanced diet …”

Section: Discussionsupporting

confidence: 85%

“…The high gut microbial diversity wileyonlinelibrary.com/journal/ps observed in shoot-feeding G. molesta may have resulted from the vast microbiota via a specific nutritional complementation from an imbalanced diet. 47,48 At the phylum level, there were no dramatic differences in the bacterial composition of the fruit-boring lepidopteran pests. Four bacterial phyla, including Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes, were predominant (relative abundance >1%).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of gut bacterial communities and their associations with host diets in four fruit borers

Liu

Shen

et al. 2019

Pest Management Science

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BACKGROUND Microbiota that live in the gut of insects have a wide range of effects on host nutrition, physiology, and behavior. They may shape the adaptation of their hosts to different habitats and lifestyles. To characterize the gut microbiota of fruit borers comprehensively, we compared bacterial communities among Grapholita molesta, Conogethes punctiferalis, Carposina sasakii, and Cydia pomonella, which are serious lepidopteran pests. We selected G. molesta as a representative pest to more explicitly test the influence of host dietary niche on the insect gut microbiome, and compared the bacterial microbial communities of G. molesta fed different diets (peach shoots and apple) using Illumina high‐throughput sequencing technology. RESULTS The results show that Proteobacteria and Firmicutes are dominant in their gut microbiota. The C. sasakii had the highest richness values and G. molesta (shoot‐feeding) had the highest diversity, whereas C. pomonella and G. molesta (fruit‐feeding) had the lowest bacterial richness and diversity, respectively. The ANOSIM analysis revealed significant differences in the structure of gut microbiota among different insects. In addition, G. molesta with a different feeding diet had significant differences in gut microbiota composition. PICRUSt analysis indicated that most functional prediction categories were related to metabolism. CONCLUSION Our results show that gut microbiota composition is affected significantly not only by host species but also host diets. An enhanced understanding of these herbivore‐associated microbial symbionts is essential for understanding the biology and ecology of the host insect, and may offer new possibilities to improve integrated pest‐management strategies for efficient control of fruit borers. © 2019 Society of Chemical Industry

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Comparison of gut bacterial communities and their associations with host diets in four fruit borers

Liu

Shen

et al. 2019

Pest Management Science

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“…Sordariomycetes fungi might assist in detoxification, as many members from this fungal group were found to be involved in organic matter decomposition and known as endophytes of plants (Santana et al ., ; Zhang et al ., ). The high abundance of Sordariomycetes were also reported in the apple buprestid Agrilus mali and Asian longhorned beetle Anoplophora glabripennis across developmental stages (Zhang et al ., ; Mason et al ., ). At the genus level, a total of 278 genera of bacteria and 80 genera of fungi were annotated in all BPH samples, including culturable and unculturable bacteria and fungi.…”

Section: Discussionmentioning

confidence: 97%

“…Remarkably, there are a large number of fungal genera not previously detected in BPH, mainly including Wallemia , Talaromyces , Cladosporium , Papiliotrema , Mucor and Penicillium . Three of them ( Wallemia , Cladosporium and Penicillium ) have also previously been identified in the apple buprestid and parasitic Dendroctonus bark beetles (Dohet et al ., ; Zhang et al ., ). Future research should focus on the prevalence and possible functions of these fungal associates of BPH.…”

Section: Discussionmentioning

confidence: 97%

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Diversity and dynamics of microbial communities in brown planthopper at different developmental stages revealed by high‐throughput amplicon sequencing

et al. 2019

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The microbiome associated with brown planthopper (BPH) plays an important role in mediating host health and fitness. Characterization of the microbial community and its structure is prerequisite for understanding the intricate symbiotic relationships between microbes and host insect. Here, we investigated the bacterial and fungal communities of BPH at different developmental stages using high‐throughput amplicon sequencing. Our results revealed that both the bacterial and fungal communities were diverse and dynamic during BPH development. The bacterial communities were generally richer than fungi in each developmental stage. At 97% similarly, 19 phyla and 278 genera of bacteria were annotated, while five fungal phyla comprising 80 genera were assigned. The highest species richness for the bacterial communities was detected in the nymphal stage. The taxonomic diversity of the fungal communities in female adults was generally at a relatively higher level when compared to other developmental stages. The most dominant phylum of bacteria and fungi at each developmental stage all belonged to Proteobacteria and Ascomycota, respectively. A significantly lower abundance of bacterial genus Acinetobacter was recorded in the egg stage when compared to other developmental stages, while the dominant fungal genus Wallemia was more abundant in the nymph and adult stages than in the egg stage. Additionally, the microbial composition differed between male and female adults, suggesting that the microbial communities in BPH were gender‐dependent. Overall, our study enriches our knowledge on the microbial communities associated with BPH and will provide clues to develop potential biocontrol techniques against this rice pest.

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Characterization of cultivable intestinal microbiota in Rhynchophorus palmarum Linnaeus (Coleoptera: Curculionidae) and determination of its cellulolytic activity

Calumby

Almeida

Barros

et al. 2022

Arch Insect Biochem Physiol

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Rhynchophorus palmarum Linnaeus is an agricultural pest that affects various palm crops, including coconut (Cocos nucifera) plantations which are prominent in the economy of Northeastern Brazil. Characterization of the intestinal microbiota of R. palmarum, as well as elucidation of aspects related to the biochemistry and physiology of the insect's digestion, is essential for intervention in specific metabolic processes as a form of pest control. Thus, this study aimed to characterize the intestinal microbiota of R. palmarum and investigate its ability to degrade cellulosic substrates, to explore new biological control measures. Intestinal dissection of eight adult R. palmarum insects was performed in a laminar flow chamber, and the intestines were homogenized in sterile phosphate‐buffered saline solution. Subsequently, serial dilution aliquots of these solutions were spread on nutritive agar plates for the isolation of bacteria and fungi. The microorganisms were identified by matrix‐assisted laser desorption/ionization with a time‐of‐flight mass spectrometry and evaluated for their ability to degrade cellulose. Fourteen bacterial genera (Acinetobacter, Alcaligenes, Arthrobacter, Bacillus, Citrobacter, Enterococcus, Kerstersia, Lactococcus, Micrococcus, Proteus, Providencia, Pseudomonas, Serratia, and Staphylococcus) and two fungal genera (Candida and Saccharomyces)—assigned to the Firmicutes, Actinobacteria, Proteobacteria, and Ascomycota phyla—were identified. The cellulolytic activity was exhibited by six bacterial and one fungal species; of these, Bacillus cereus demonstrated the highest enzyme synthesis (enzymatic index = 4.6). This is the first study characterizing the R. palmarum intestinal microbiota, opening new perspectives for the development of strategies for the biological control of this insect.

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Bacterial and fungal gut communities of Agrilus mali at different developmental stages and fed different diets

Cited by 48 publications

References 61 publications

Comparison of gut bacterial communities and their associations with host diets in four fruit borers

Comparison of gut bacterial communities and their associations with host diets in four fruit borers

Diversity and dynamics of microbial communities in brown planthopper at different developmental stages revealed by high‐throughput amplicon sequencing

Characterization of cultivable intestinal microbiota in Rhynchophorus palmarum Linnaeus (Coleoptera: Curculionidae) and determination of its cellulolytic activity

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