Bacterial and fungal microleakage of AH26 and AH Plus root canal sealers

Miletić, Ivana; Prpić-Mehičić, Goranka; Maršan, Teuta; Tambić-Andrašević, Arjana; Pleško, Sanja; Karlović, Zoran; Anić, Ivica

doi:10.1046/j.1365-2591.2002.00490.x

Cited by 26 publications

(27 citation statements)

References 18 publications

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“…The fungus Candida albicans has the same capacity as bacteria to penetrate filled canals (10). Polymicrobial infections involving black-pigmented Gramnegative anaerobes are likely to have a greater pathogenic potential than infections with a single species (40).…”

Section: Bacteria and Toxin Infiltration Methodsmentioning

confidence: 99%

“…Commonly used tracers are dyes, radioisotopes, bacteria and their products, such as endotoxins. Bacteria used as tracers most closely approximate what happens clinically in terms of leakage (9)(10)(11). Other methodologies, such as fluid filtration and dye extraction methods have also been used, their main advantage being high reproducibility (1,(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

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Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review

Veríssimo

Vale

2006

Journal of Oral Science

137

110

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Keywords: dye dilution technique; dyes; filtration. IntroductionMicroorganisms present inside root canals may remain active in the dentinal tubules even after vigorous chemicalmechanical preparation. Thus, perfect apical sealing is desirable to prevent the remaining bacteria and their endotoxins from reaching the root apex (1,2). Apical leakage is considered to be a common cause for endodontic therapy failure, and is influenced by many variables such as different filling techniques, the physical and chemical properties of sealers and the presence or absence of a smear layer (1,3). In coronal leakage, the canal may be recontaminated in various ways such as contact between the oral bacterial flora and root canal tubule inlets. However, it most frequently occurs as a result of loss of temporary filling, or inadequate endodontic filling or crown sealing (4,5).According to Timpawat et al. (6), endodontic sealers are used to eliminate the interface between the gutta-percha and the dentinal walls. Leakage may, however, occur at the interfaces between the sealer and dentin, sealer and gutta-percha and in spaces within the sealer itself. Thus, the quality of the filling depends largely on the sealing capacity offered by sealers (7,8).Methodologies in vitro are used to estimate sealing quality, generally by measuring microleakage that allows the tracer agent to penetrate the filled canal. Commonly used tracers are dyes, radioisotopes, bacteria and their products, such as endotoxins. Bacteria used as tracers most closely approximate what happens clinically in terms of leakage (9-11). Other methodologies, such as fluid filtration and dye extraction methods have also been used, their main advantage being high reproducibility (1,(12)(13)(14).The aim of this review was to critically analyze the various methodologies reported in the literature for assessing root canal sealing capacity. Apparently, they are not standardized, which makes it difficult to compare the results obtained when different methodologies are used, even though the same substrate is being assessed. Literature ReviewAlthough there are many leakage studies, there is no consensus about the endodontic sealer and core material sealing capacities. One of the reasons is that investigations do not use a standardized methodology and this frequently

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Section: Bacteria and Toxin Infiltration Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review

Veríssimo

Vale

2006

Journal of Oral Science

137

110

View full text Add to dashboard Cite

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“…Trabalhos, como os de Jacobson et al 9 (2002), Miletić et al 10 (2002) e Gomes-Filho et al 11 (2012), demonstram que somente a obturação de canal, após o tratamento endodôntico, não é o suficiente para impedir a penetração de microrganismos até a região periapical, permitindo a percolação ou a microinfiltração coronária, resultando na recontaminação dos tecidos periapicais.…”

Section: Discussionunclassified

Infiltração marginal microbiana em selamento coronário duplo

Parron

Panerari

Cimardi

et al. 2014

Rev. odontol. UNESP

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ResumoIntrodução: O selamento coronário deve ser eficiente em impedir a penetração de saliva e seus contaminantes para o interior do sistema de canais radiculares. Objetivo: Avaliar o grau de infiltração microbiana em selamento duplo coronário utilizando Coltosol  associado ao MaxxionR  e ao Bioplic  . Material e método: Foram utilizados dentes pré-molares humanos extraídos, nos quais foram realizadas aberturas coronárias padronizadas com seis milímetros de profundidade. Os grupos foram divididos de acordo com os cimentos restauradores provisórios: Grupo I -Bioplic  ; Grupo II: Bioplic  + Coltosol  ; Grupo III: Maxxion R  ; Grupo IV: Maxxion R  + Coltosol  ; Grupo V -Coltosol  ; Grupo controle. Foi confeccionado um dispositivo adaptando-se os dentes na porção inferior de tubos Eppendorf  , de modo que dois terços se projetem para fora do tubo plástico. O dispositivo foi fixado em frasco de vidro, contendo 7 mL de caldo estéril "Brain Heart Infusion". Na porção superior do tubo Eppendorf, foi realizada a inoculação de Enterococcus faecalis. A leitura foi realizada a cada 24h, durante 30 dias, avaliando-se a turvação no meio de cultura em contato com o ápice dentário. Os resultados foram submetidos ao teste estatístico Kruskal-Wallis (p<0,05). Resultado: Houve infiltração em todos os grupos ao final dos 30 dias e os grupos I e IV apresentaram 50% de infiltração. Os grupos IV e V apresentaram infiltração em cinco dias e o grupo III, em 26 dias. Conclusão: A associação entre os cimentos restauradores provisórios não impediu nem diminuiu a infiltração marginal de Enterococcus faecalis.Descritores: Infiltração dentária; restauração dentária temporária; Enterococcus faecalis. AbstractIntroduction: The coronal sealing should be effective in preventing the penetration of saliva and its contaminants into the root canal system. Objective: To evaluate the degree of microbial coronal leakage in double sealing using Coltosol  associated MaxxionR  and Bioplic  . Material and method: premolars extracted human teeth were used, in which standardized with 6 mm deep coronary openings will be held . The groups were divided according to provisional restorative cements : Group I -Bioplic  ; Group II -Bioplic  +Coltosol  ; Group III -Maxxion R  ; Group IV -Maxxion R  + Coltosol  ; Group V -Coltosol  ; Control Group. One adapting the teeth in the lower portion of Eppendorf  tubes device was manufactured so that two-thirds to protrude out of the plastic tube. The device was fixed to a glass vial containing 7 ml of sterile broth "Brain Heart Infusion". In the upper portion of the Eppendorf tube was inoculated with Enterococcus faecalis performed. The reading was performed every 24 hours for 30 days evaluating turbidity in the culture medium in contact with the tooth apex. The results were analyzed by Kruskal -Wallis test (p < 0.05 ) statistical test. Result: There was infiltration in all groups at the end of 30 days, and groups I and IV showed 66% infiltration. Groups IV and V showed infiltration in 5 days and group ...

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“…[12], -Bakteriellen Dichtigkeit [4,[13][14][15][16][17], -Penetration radioaktiver Isotope [18], -Penetration von wässrigen Farbstofflösungen [10,[19][20][21], -Flüssigkeits-Transport and glucose penetration model [22][23][24][25]. Die sensitivste Untersuchungsmethode von all diesen Methoden zur Beurteilung der apikalen Dichtigkeit ist die Farbstoffpenetrationsmethode [26].…”

Section: Ziel Der Arbeitunclassified

Dichtigkeit von Wurzelkanalfüllungen mit GuttaFlow® und Resilon™ im Vergleich zur lateralen Kondensation

Kqiku

Miletić

Gruber

et al. 2010

Wien Med Wochenschr

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Epiphany/Resilon and GuttaFlow are newly developed methods for obturation of the root canal system. Epiphany/Resilon is a thermoplastic, synthetic polymer-based root canal filling material which enables the bonding to the dentin root canal wall during root canal obturation. GuttaFlow is a cold flowable filling system for the obturation of root canals, combining sealer and gutta-percha in one product. The purpose of this study was to assess the leakage of the Epiphany/Resilon or GuttaFlow root canal filling compared with lateral condensation of gutta-percha. For this study were used 45 human extracted teeth, chemo mechanically prepared, divided into three groups and obturated with gutta-percha/AH Plus, Epiphany/Resilon and GuttaFlow. For dye penetration all teeth were centrifuged for three minutes at 30 g in 2% methylene blue and dissolved in 65% nitric acid for 3 days. The extracted methylene blue was determined with Photometer. Root Canal fillings with Epiphany/Resilon showed less dye penetration than lateral condensation of gutta-percha and GuttaFlow. Epiphany/Resilon is ideally suited as a root canal filling material.

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Bacterial and fungal microleakage of AH26 and AH Plus root canal sealers

Abstract: In this in vitro study, gutta-percha and the sealers AH26 and AH Plus allowed leakage of bacteria and fungi.

Cited by 26 publications

References 18 publications

Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review

Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review

Infiltração marginal microbiana em selamento coronário duplo

Dichtigkeit von Wurzelkanalfüllungen mit GuttaFlow® und Resilon™ im Vergleich zur lateralen Kondensation

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