Bacterial and protozoan pathogens/symbionts in ticks infecting wild grasscutters (Thryonomys swinderianus) in Ghana

Adenyo, Christopher; Ohya, Kenji; Qiu, Yongjin; Takashima, Yasuhiro; Ogawa, Hirohito; Matsumoto, Tateki; Thu, May June; Sato, Kozue; Kawabata, Hiroki; Katayama, Yukie; Omatsu, Tsutomu; Mizutani, Tetsuya; Fukushi, Hideto; Katakura, Ken; Nonaka, Narikaki; Inoue‐Murayama, Miho; Kayang, Boniface B.

doi:10.1016/j.actatropica.2020.105388

Cited by 10 publications

(9 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although previous studies reported the presence of C. pecorum in birds, most reports are from captive birds [6,24]. Therefore, to confirm the presence of C. pecorum in the wild birds of the Afadjato area, we attempted to analyze the 16S rRNA region using pan-Chlamydiales PCR that could detect a broad range of Chlamydiales, including parachlamydia, with a high sensitivity and a detection limit of 5 copies as described previously [1,16]. In this pan-Chlamydiales PCR, we used locked nucleic acids in the PCR primers to improve PCR performance [1,16].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, to confirm the presence of C. pecorum in the wild birds of the Afadjato area, we attempted to analyze the 16S rRNA region using pan-Chlamydiales PCR that could detect a broad range of Chlamydiales, including parachlamydia, with a high sensitivity and a detection limit of 5 copies as described previously [1,16]. In this pan-Chlamydiales PCR, we used locked nucleic acids in the PCR primers to improve PCR performance [1,16]. With this improved PCR method, we successfully constructed a phylogenetic tree of partial 16S rRNA sequences.…”

Section: Discussionmentioning

confidence: 99%

“…The whole-genome amplification of positive samples was conducted using an illustra GenomiPhi DNA Amplification kit (GE healthcare, Chicago, IL, USA) to enhance the PCR efficiency. The ompA VD2 region was amplified using nested PCR with an outer pair of primers (CMGP-1F: 5′-CCTTGTGATCCTTGCGCTACTTG-3′/CMGP-1R: 5′-GTGAGCAGCTCTTCGTTGAT-3′) and an inner pair of primers (CMGP-2F: 5′-GCCTTAAACATCTGGGATCG-3′/CMGP-2R: 5′-GCACAACCACATTCCCATAAAG-3′) and previously described conditions [3] Chlamydiales, including parachlamydia, with high sensitivity and a detection limit of 5 copies [1,16]. The enoA gene was amplified with the primers YPenoA5: 5′-CCWATGATGAAYCTYATTAACGG-3′ and YPenoA6: 5′-TCTTCYTCAGGRAGGCCATCT-3′, (W = A or T; Y = C or T), following a method described elsewhere [22].…”

Section: Phylogenetic Analyses Of Detected Species Of Family Chlamydi...mentioning

confidence: 99%

See 2 more Smart Citations

Chlamydial species among wild birds and livestock in the foothills of Mt. Afadjato, Ghana

Sassa-O’Brien

Ohya

Yasuda-Koga

et al. 2022

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

The members of family Chlamydiaceae have a broad host range and cause many kinds of diseases in humans and animals. Several cases of Chlamydiaceae being detected in atypical hosts have been reported recently. Consequently, cross-species monitoring of Chlamydia in wildlife and livestock is pertinent for public health, animal hygiene and wildlife conservation. In this study, we conducted molecular surveillance of Chlamydia in wild birds and livestock around a small village in the foothills of Mt. Afadjato, Ghana where direct contact between wildlife and livestock occurs. Among 29 captured wild birds and 63 livestock, 5 sheep, 30 goats and 28 chickens, the positive ratios of Chlamydia were 24.1%, 40.0%, 43.3% and 26.9%, respectively. Chlamydia pecorum was detected in wild birds, goats, sheep and chickens. On the basis of the variable domain 2 region of ompA, several samples from different hosts showed identical sequences and were phylogenetically located to the same clusters. In addition, using ompA, C. psittaci, C. abortus and C. gallinacea were also detected in this small habitat. Further genetic and pathogenic analyses of the chlamydial distribution in this area, which represents the interface of wild and domestic animal interactions, may improve our knowledge of their transmission among different hosts.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Phylogenetic Analyses Of Detected Species Of Family Chlamydi...mentioning

confidence: 99%

See 1 more Smart Citation

Chlamydial species among wild birds and livestock in the foothills of Mt. Afadjato, Ghana

Sassa-O’Brien

Ohya

Yasuda-Koga

et al. 2022

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…(10 positive/44 tested pools; 23%) and the more sporadic presence of Bartonella spp. (4/44; 9%) in grasscutter I. aulacodi ticks should alert public health managers, since these tick-borne pathogens could pose a major threat to people whose livelihoods depend on animal production and in particular grasscutter farms, which have been set up to meet the high demand for its meat in the local market (Adenyo et al, 2020). However, studies must be carried out to identify the species of these pathogens incriminated.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Rickettsia spp., Bartonella spp., and Anaplasma phagocytophilum in hard ticks collected from wild animals in Benin, West Africa

Yessinou

Adehan

Hedegbetan³

et al. 2022

Trop Anim Health Prod

View full text Add to dashboard Cite

Ticks and tick-borne pathogens constitute a growing veterinary and public health concern around the world. Ticks are considered to be natural reservoirs for tick borne related pathogens and are equally responsible for the spread of infections in animals as well as in humans. In this study, the presence of Rickettsia and Bartonella species and Anaplasma phagocytophilum was investigated in hard ticks collected from reptiles, birds and wild mammalian animals. The samples collection of this study was carried out between December 2020 and September 2021. Adult ticks (male and female) were collected from wild animals in six districts of southern Benin. Molecular analysis was used to verify the presence of pathogens in all the samples (ticks) collected from wild animals. A total of 504 ticks were collected and grouped into 115 different tick pools. The PCR analysis detected 19 out of 115 tick pools positive for Rickettsia spp. and 9/115 tick pools positive for Bartonella spp., while Anaplasma phagocytophilum DNA was not detected in any tick. Several of the tick species collected from our studied reptiles/wild mammalian animals could be potential sources of zoonotic pathogens when subjected to further investigation. Therefore, stringent attention should be paid to tick infestation of reptiles/wild mammalian animals in order to put in place proper control and prevention measures for tick-borne diseases in the wild, which could serve as reservoirs in the infestation of domestic animals/humans in the event of any possible contact.

show abstract

“…The PCR was conducted as described above with the annealing temperatures listed in Table 1 . Tick DNA samples positive for Anaplasma in our previous study [ 27 , 28 ] and sterilised water were included in each PCR run as positive and negative controls, respectively. The PCR products were separated by electrophoresis on a 1.0% agarose gel stained with Gel-Red and visualised under UV light ( Supplementary Figure S1 ).…”

Section: Methodsmentioning

confidence: 99%

Molecular Survey of Babesia and Anaplasma Infection in Cattle in Bolivia

Ogata

Pereira

Vega

et al. 2021

Veterinary Sciences

Self Cite

View full text Add to dashboard Cite

Latin American countries produce more than a quarter of the world’s beef and are a major global supplier of livestock protein. Tick-borne diseases (TBDs) are a major constraint to the livestock industry worldwide, including in Latin America. The aim of this study was to detect and characterise tick-borne pathogens in cattle from Santa Cruz, Bolivia, where no detailed epidemiological data are available. Blood samples were collected from 104 cattle. Apicomplexan parasites were detected by nested PCR amplification of the 18S ribosomal RNA gene (rDNA), and Anaplasmataceae was screened by the PCR amplification of 16S rDNA, followed by characterisation based on the heat shock protein and citrate synthase gene sequences. Babesia infection was observed in nine cattle (one Babesia bovis and eight Babesia bigemina), while Anaplasmataceae infection was detected in thirty-two cattle. A sequencing analysis confirmed the presence of Anaplasma marginale and Anaplasma platys-like. These results provide the first molecular evidence for the four above-mentioned tick-borne pathogens in cattle in Bolivia. This information improves our understanding of the epidemiology of TBDs and will help in formulating appropriate and improved pathogen control strategies.

show abstract

Bacterial and protozoan pathogens/symbionts in ticks infecting wild grasscutters (Thryonomys swinderianus) in Ghana

Cited by 10 publications

References 40 publications

Chlamydial species among wild birds and livestock in the foothills of Mt. Afadjato, Ghana

Chlamydial species among wild birds and livestock in the foothills of Mt. Afadjato, Ghana

Molecular characterization of Rickettsia spp., Bartonella spp., and Anaplasma phagocytophilum in hard ticks collected from wild animals in Benin, West Africa

Molecular Survey of Babesia and Anaplasma Infection in Cattle in Bolivia

Contact Info

Product

Resources

About