Bacterial Behavior

Armitage, Judith P.

doi:10.1007/0-387-30742-7_5

Cited by 5 publications

(2 citation statements)

References 344 publications

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“…had only one gene ( fliC ) encoding the filament. Although the Rhizobiaceae have mostly peritrichous flagella (Armitage, 2004), Brucella , much more like C. crescentus , appears to build a polar (monotrichous) flagellum, at least in the condition tested. Finally, as demonstrated by the immunogold labelling with anti‐LPS O‐chain monoclonal antibody (mAb), Brucella has a sheathed flagellum similar to that described in V. cholerae (Fuerst and Perry, 1988), Bdellovibrio sp.…”

Section: Discussionsupporting

confidence: 88%

“…Several points make the flagellum of Brucella quite peculiar among Rhizobiaceae or even alphaproteobacteria. As opposed to the majority of bacterial species which have flagellar filaments made from a single protein (flagellin) (Armitage, 2004), the alphaproteobacteria (e.g. Agrobacterium tumefaciens , C. crescentus , S. meliloti , Rhizobium lupini , Rhodobacter sphaeroides ) have various flagellins as subunits to construct a complex rigid flagellum (Scharf et al ., 2001; Armitage, 2004).…”

Section: Discussionmentioning

confidence: 99%

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The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infection

Frétin

Fauconnier

Köhler

et al. 2005

Cellular Microbiology

143

188

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SummaryPersistence infection is the keystone of the ruminant and human diseases called brucellosis and Malta fever, respectively, and is linked to the intracellular tropism of Brucella spp. While described as nonmotile, Brucella spp. have all the genes except the chemotactic system, necessary to assemble a functional flagellum. We undertook to determine whether these genes are expressed and are playing a role in some step of the disease process. We demonstrated that in the early log phase of a growth curve in 2YT nutrient broth, Brucella melitensis expresses genes corresponding to the basal (MS ring) and the distal (hook and filament) parts of the flagellar apparatus. Under these conditions, a polar and sheathed flagellar structure is visible by transmission electron microscopy (TEM). We evaluated the effect of mutations in flagellar genes of B. melitensis encoding various parts of the structure, MS ring, P ring, motor protein, secretion apparatus, hook and filament. None of these mutants gave a discernible phenotype as compared with the wild-type strain in cellular models of infection. In contrast, all these mutants were unable to establish a chronic infection in mice infected via the intraperitoneal route, raising the question of the biological role(s) of this flagellar appendage.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infection

Frétin

Fauconnier

Köhler

et al. 2005

Cellular Microbiology

143

188

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Bacterial Behavior

Armitage¹,

Scott²

2013

The Prokaryotes

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Ammonium and attachment of Rhodopirellula baltica

et al. 2011

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A dimorphic life cycle has been described for the planctomycete Rhodopirellula baltica SH1(T), with juvenile motile, free-swimming cells and adult sessile, attached-living cells. However, attachment as a response to environmental factors was not investigated. We studied the response of R. baltica to nitrogen limitation. In batch cultures, ammonium limitation coincided with a dominance of free-swimming cells and a low number of aggregates. Flow cytometry revealed a quantitative shift with increasing ammonium availability, from single cells towards attached cells in large aggregates. During growth of R. baltica on glucose and ammonium in chemostats, an ammonium addition caused a macroscopic change of the growth behaviour, from homogeneous growth in the liquid phase to a biofilm on the borosilicate glass wall of the chemostat vessel. Thus, an ammonium limitation-a carbon to nitrogen supply ratio of 30:1-sustained free-living growth without aggregate formation. A sudden increase in ammonium supply induced sessile growth of R. baltica. These observations reveal a response of Rhodopirellula baltica cells to ammonium: they abandon the free-swimming life, attach to particles and form biofilms.

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Bacterial Behavior

Cited by 5 publications

References 344 publications

The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infection

The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infection

Bacterial Behavior

Ammonium and attachment of Rhodopirellula baltica

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