Bacterial biodiversity from an anaerobic up flow bioreactor with ANAMMOX activity inoculated with swine sludge

Viancelli, Aline; Kunz, Airton; Esteves, Paulo Augusto; Bauermann, Fernando V.; Furukawa, Koichi; Fujii, Tomoyuki; Antônio, Regina Vasconcellos; Vanotti, Matías B.

doi:10.1590/s1516-89132011000500022

Cited by 28 publications

(7 citation statements)

References 17 publications

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“…Previous phylogenetic analysis based on 16S rRNA genes indicated that Jettenia was most closely related (98% similarity of 16S rRNA gene) to an anammox bacterium strain called KSU-1 (Quan et al, 2008 ; Viancelli et al, 2011 ). Additionally, the HZS subunit A ( hzsA ), a phylomarker for anammox bacteria, of Jettenia and KSU-1 shared 92.8% sequence identity on nucleotide level and 97.0% on amino acid level, which was much higher than any other pair of anammox bacteria (Harhangi et al, 2012 ).…”

Section: Methodsmentioning

confidence: 99%

Metagenome Analysis of a Complex Community Reveals the Metabolic Blueprint of Anammox Bacterium “Candidatus Jettenia asiatica”

Speth

Françoijs

et al. 2012

Front. Microbio.

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Anaerobic ammonium-oxidizing (anammox) bacteria are key players in the global nitrogen cycle and responsible for significant global nitrogen loss. Moreover, the anammox process is widely implemented for nitrogen removal from wastewaters as a cost-effective and environment-friendly alternative to conventional nitrification-denitrification systems. Currently, five genera of anammox bacteria have been identified, together forming a deep-branching order in the Planctomycetes-Verrucomicrobium-Chlamydiae superphylum. Members of all genera have been detected in wastewater treatment plants and have been enriched in lab-scale bioreactors, but genome information is not yet available for all genera. Here we report the metagenomic analysis of a granular sludge anammox reactor dominated (∼50%) by “Candidatus Jettenia asiatica.” The metagenome was sequenced using both Illumina and 454 pyrosequencing. After de novo assembly 37,432 contigs with an average length of 571 nt were obtained. The contigs were then analyzed by BLASTx searches against the protein sequences of “Candidatus Kuenenia stuttgartiensis” and a set of 25 genes essential in anammox metabolism were detected. Additionally all reads were mapped to the genome of an anammox strain KSU-1 and de novo assembly was performed again using the reads that could be mapped on KSU-1. Using this approach, a gene encoding copper-containing nitrite reductase NirK was identified in the genome, instead of cytochrome cd1-type nitrite reductase (NirS, present in “Ca. Kuenenia stuttgartiensis” and “Ca. Scalindua profunda”). Finally, the community composition was investigated through MetaCluster analysis, 16S rRNA gene analysis and read mapping, which showed the presence of other important community members such as aerobic ammonia-oxidizing bacteria, methanogens, and the denitrifying methanotroph “Ca. Methylomirabilis oxyfera”, indicating a possible active methane and nitrogen cycle in the bioreactor under the prevailing operational conditions.

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Section: Methodsmentioning

confidence: 99%

Metagenome Analysis of a Complex Community Reveals the Metabolic Blueprint of Anammox Bacterium “Candidatus Jettenia asiatica”

Speth

Françoijs

et al. 2012

Front. Microbio.

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“…In studies by VIANCELLI et al (2011), in which partial nitritation and anammox activity occurred in the same reactor submitted to DO values lower than 0.5 mg L −1 , process viability was confirmed under low dissolved oxygen concentration. In experiments with complete nitrification, ANDRADE et al (2010) assessed the influence of DO on nitrification speed and observed that nitrification rate is directly proportional to DO concentration.…”

Section: Introductionmentioning

confidence: 92%

“…The factors that exert a greater influence on this inhibition are pH, dissolved oxygen (DO), temperature and nitrifying bacteria (Nitrosomonas and Nitrobacter) doubling rate (PRA et al, 2012;VIANCELLI et al, 2011;SUN et al 2010, ZHU et al 2008.…”

Section: Introductionmentioning

confidence: 99%

Stability of Partial Nitritation of Poultry Slaughterhouse Wastewater in a Sequential Batch Reactor

et al. 2017

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ABSTRACT:The aim of this study was to monitor the stabilization of a sequential batch reactor (SBR) regarding the partial nitritation of poultry slaughterhouse wastewater, aiming at preparing it for a reactor with bacteria that promote anaerobic ammonia oxidation (anammox). A cylindrical reactor with a useful volume of 3.5 L, mechanical agitation (50 rpm), and suspended biomass was used in the experiment. The strategies adopted for nitrite-oxidizing bacteria (NOB) inhibition were cycle time (CT: 24, 16, 6, 5, 4 h and real-time monitoring), free ammonia accumulation, alkalinity restriction and low concentrations of dissolved oxygen (DO). The ammonification process, observed at all experimental phases, negatively influenced the control of partial nitritation process. Alkalinity restriction under low cycle times (4 and 5 h) conditions, together with low dissolved oxygen concentration, was a key factor for nitrite-oxidizing bacteria inhibition. Stability of partial nitritation was only reached by real-time monitoring, being the cycles stopped when 50% N-NH4 + and 50% N-NO2 − were present in the reactor.

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“…Sejak penemuannya pada tahun 1995, proses anammox dalam siklus nitrogen biologis terus dilakukan eksplorasi. Saat ini, terdapat 7 genus bakteri anammox, yaitu Candidatus Brocadia (6) , Candidatus Kuenenia (7) , Candidatus Scalindua (8) , Candidatus Anammoxoglobus (9) , Candidatus Jettenia (10) , Canditatus Brasilis (11) dan Candidatus Anammoximicrobium (12) dengan jumlah 22 spesies (13) akan tetapi tidak satupun genus atau spesies yang dilaporkan ditemukan di Indonesia. Indonesia sebagai negara tropis yang terkenal dengan keanekaragaman hayati dan mikroba memiliki potensi ditemukannya bakteri anammox.…”

Section: Latar Belakangunclassified

Start – Up Proses Anammox Menggunakan Lumpur Telaga Kotobaru sebagai Inokulum

2020

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The anammox process plays an essential role in removing nitrogen from the waters anaerobically. Since the discovery of anammox in 1995, no studies have reported anammox bacteria from the Indonesian environment. This research aims to begin exploring anammox bacteria from the environment in Indonesia as a tropical country. The exploration was carried out with a start-up anammox process in a continuous reactor. The reactor was constructed using a housing filter equipped with a string wound filter as a supporting media for biofilm. Sludge from Koto Baru Lake, Tanah Datar, Indonesia, was used as inoculum. The substrate was fed into the reactor through the inside of the filter using a peristaltic pump. Ammonium and nitrite were supplemented to the substrate at a 70-150 mg-N/L concentration and operated at room temperature. The samples were collected once a week. Ammonium and nitrite were measured using the colorimetric method, nitrate using the ultraviolet spectrophotometric method. Performance of nitrogen removal and the growth biofilm in the reactor shown the success of the start of the anammox process. After 140 days of reactor operation, the maximum value of nitrogen removal rate (NRR) was 0.271 kg-N/m3.day at the nitrogen loading rate (NLR) 0.3095 kg-N/m3.day. Ammonium conversion efficiency (ACE) and nitrogen removal efficiency (NRE) during start-up were 97.07% and 91.92%. Red biofilm growth on the filter and the reactor's inner wall, which is characteristic of the anammox bacteria biomass.Keywords: anammox, Indonesia, Koto Baru Lake, tropical ABSTRAKProses anammox memiliki peran penting dalam penyisihan nitrogen dari perairan secara anaerobik. Sejak ditemukannya anammox pada 1995, belum ada penelitian yang melaporkan keberadaan bakteri anammox dari lingkungan Indonesia. Tujuan dari penelitian ini adalah untuk memulai eksplorasi bakteri anammox dari lingkungan di Indonesia sebagai negara tropis. Eksplorasi dilakukan dengan start-up proses anammox pada sebuah reaktor kontinu. Reaktor terdiri dari housing filter yang dilengkapi dengan string wound filter sebagai media lekat untuk biofilm. Lumpur dari Telaga Koto Baru digunakan sebagai inokulum. Substrat dialirkan ke dalam reaktor melalui bagian dalam filter menggunakan pompa peristaltik. Amonium dan nitrit ditambahkan ke substrat dengan konsentrasi 70-150 mg-N/L dan dioperasikan pada suhu kamar. Konsentrasi amonium dan nitrit diukur dengan metode kolorimetri, serta konsentrasi nitrat dianalisis menggunakan metode spektrofotometri UV dengan interval pengukuran sampel setiap 5 hari. Pengamatan penyisihan nitrogen dan pertumbuhan biofilm di reaktor menunjukkan keberhasilan dimulainya proses anammox. Setelah 140 hari operasional reaktor, didapatkan nilai tingkat penyisihan nitrogen (TPyN) maksimum 0,271 kg-N/m3.hari pada tingkat pemuatan nitrogen (TPN) 0,3095 kg-N/m3.hari. Nilai efisiensi konversi amonium (EKA) dan efisiensi penyisihan nitrogen (EPN) maksimum selama start-up adalah : 97,07% dan 91,92%. Biofilm berwarna merah tumbuh pada filter dan dinding bagian dalam reaktor yang merupakan karakteristik dari biomasa bakteri anammox.Kata kunci: anammox, Indonesia, Telaga Koto Baru, tropis

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Bacterial biodiversity from an anaerobic up flow bioreactor with ANAMMOX activity inoculated with swine sludge

Abstract: ABSTRACT

Cited by 28 publications

References 17 publications

Metagenome Analysis of a Complex Community Reveals the Metabolic Blueprint of Anammox Bacterium “Candidatus Jettenia asiatica”

Metagenome Analysis of a Complex Community Reveals the Metabolic Blueprint of Anammox Bacterium “Candidatus Jettenia asiatica”

Stability of Partial Nitritation of Poultry Slaughterhouse Wastewater in a Sequential Batch Reactor

Start – Up Proses Anammox Menggunakan Lumpur Telaga Kotobaru sebagai Inokulum

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