Bacterial bioluminescence inhibition by Chlorophenols

Ismailov, A. D.; Pogosyan, S. I.; Mitrofanova, T. I.; Егоров, Н.С.; Нетрусов, А.И.

doi:10.1007/bf02738051

Cited by 7 publications

(6 citation statements)

References 17 publications

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“…In the case of PCP, the most pronounced distinctions in the inhibitory effect depending on pH were observed; in the case of 2,4-D, the least. The sensitivities of both species to chlorophenols increased in the order 2,4-D, 2,4,5-T, and PCP, which accords with published data [6,10]. The efficiency of luminescence inhibition by 2,6-DMP is similar to the effect of 2,4-D at neutral pH values, thereby suggesting that EC 10 and EC 50 values are similar.…”

Section: Inhibitory Effect Of Chlorophenols At Various Phsupporting

confidence: 90%

“…The activation by chlorophenols was reported earlier [6]. Other aromatic xenobiotics have displayed similar effects [22].…”

Section: Inhibitory Effect Of Chlorophenols At Various Phsupporting

confidence: 60%

“…As was demonstrated in experiments with Escherichia coli cells carrying cloned luciferase genes [10], the toxic effect increased with the number of chlorine atoms in the chlorophenol molecule. The same pattern was observed in analyzing the effect of chlorinated phenols and phenoxyacetic acids on Vibrio harveyi cells [6]. Note that the enzymatic complexes of the bioluminescent system are associated with the bacterial plasma membrane [11].…”

supporting

confidence: 60%

“…The mechanism of action of chlorophenols on bacterial structures is an important topic not only for biodetection but also for an understanding of the processes involved in microbiological degradation of these substances [4,5]. Both the interaction of a toxicant with bacterial luciferase and the mediated response of the bioluminescent system to other processes (first and foremost, those controlling the formation of luciferase substrates-reduced flavin and long-chain aliphatic aldehyde) may determine the effect of xenobiotics on the luminescence of bacterial cells [6].Many papers [7-9] consider the plasma membrane as the primary target of chloroaromatic toxicants. Absorption of toxicants changes the physical characteristics of the membrane, which disrupts membrane functions and, correspondingly, influences cell viability and growth [9].…”

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confidence: 99%

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Inhibitory Effects of Phenolic Ecotoxicants on Photobacteria at Various pH Values

IuM

et al. 2005

Appl Biochem Microbiol

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An integral concept of the primary and secondary targets for chlorophenolic toxicants in luminescent bacteria has not been developed, in spite of the fact that photobacteria are used on a large scale as biosensors for these and other toxic compounds [1][2][3]. As a rule, the main attention is paid to comparative analysis of toxicities of certain classes of chemical compounds versus structural parameters of their molecules [2,3]. The mechanism of action of chlorophenols on bacterial structures is an important topic not only for biodetection but also for an understanding of the processes involved in microbiological degradation of these substances [4,5]. Both the interaction of a toxicant with bacterial luciferase and the mediated response of the bioluminescent system to other processes (first and foremost, those controlling the formation of luciferase substrates-reduced flavin and long-chain aliphatic aldehyde) may determine the effect of xenobiotics on the luminescence of bacterial cells [6].Many papers [7-9] consider the plasma membrane as the primary target of chloroaromatic toxicants. Absorption of toxicants changes the physical characteristics of the membrane, which disrupts membrane functions and, correspondingly, influences cell viability and growth [9]. The fact that the efficiency of chlorophenolic toxicants increases with the hydrophobicity of their molecule is interpreted as a proof of the membrane action of these compounds. As was demonstrated in experiments with Escherichia coli cells carrying cloned luciferase genes [10], the toxic effect increased with the number of chlorine atoms in the chlorophenol molecule. The same pattern was observed in analyzing the effect of chlorinated phenols and phenoxyacetic acids on Vibrio harveyi cells [6]. Note that the enzymatic complexes of the bioluminescent system are associated with the bacterial plasma membrane [11]. The damage of the membrane structural and functional organization by chlorophenols should in any case influence the cell luminescent reaction.The organochlorinated compounds chosen for this work, namely, 2,4-di-and 2,4,5-triphenoxyacetic acids (2,4-D and 2,4,5-T), belong to the so-called bifunctional inhibitors [12] with the properties of weak acids (pK ~ 2.6 ), possessing a hydrophobic aromatic core and a carboxyl group. As a rule, such compounds interact with biological structures via the aromatic core, followed by the reaction of the acid group with the corresponding binding centers. The hydrophobicity of the toxin molecule is the determining parameter. The efficiency of such compounds depends on the pH of the medium; moreover, the pH influences the energy and metabolic potential of the cell, on the one hand, thereby affecting emission kinetics and intensity, and the dissociation of the toxicant and the membrane surface charge, on the other hand. The combination of these processes may influence the binding of the toxicant and its transfer into the cell.Only sparse data about the effect of pH on the toxicity of chlorophenols may be found in the lit...

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Section: Inhibitory Effect Of Chlorophenols At Various Phsupporting

confidence: 90%

“…The activation by chlorophenols was reported earlier [6]. Other aromatic xenobiotics have displayed similar effects [22].…”

Section: Inhibitory Effect Of Chlorophenols At Various Phsupporting

confidence: 60%

supporting

confidence: 60%

mentioning

confidence: 99%

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Inhibitory Effects of Phenolic Ecotoxicants on Photobacteria at Various pH Values

IuM

et al. 2005

Appl Biochem Microbiol

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“…Light production is directly proportional to the metabolic activity of the bacterial population and any inhibition of enzymatic activity causes a corresponding decrease in bioluminescence . With regard to the mechanism of inhibition of bioluminescent activity developed by BE in the presence of toxicants, it can be argued that chlorophenol compounds with a pronounced cytotoxic effect both disrupt the normal functioning of mitochondria and cytoplasmic membranes and interfere with the normal course of energy processes .…”

Section: Resultsmentioning

confidence: 99%

Biosensitive element in the form of immobilized luminescent photobacteria for detecting ecotoxicants in aqueous flow‐through systems

et al. 2016

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We demonstrated the possibility of long-term and efficient application of a biosensitive element (BE) in the form of Photobacterium phosphoreum photobacteria immobilized in poly(vinyl alcohol) (PVA) cryogel for detecting various ecotoxicants (Zn(2) (+) , Cu(2) (+) , Hg(2) (+) , Pb(2) (+) , 2,4-dichlorophenoxyacetic acid, 2,6-dimethylphenol, pentachlorophenol, coumaphos, malathion, chlorpyrifos and methyl parathion) in flow-through media. The range of detectable concentrations of ecotoxicants was determined at 1 × 10(-8) to 1 × 10(-4) M for heavy metal ions and at 1 × 10(-8) to 1 × 10(-5) M for phenol derivatives and organophosphorus pesticides. Immobilized cells of photobacteria quantitatively reacted with these ecotoxicants; cell sensitivity exhibited no flow rate dependence in the range from 45 to 180 mL/h. At a constant concentration of ecotoxicant in the flow, the bioluminescence quenching profile of immobilized cells demonstrated an integral response. The BE could remain in a flow-through medium for at least 10 days while retaining 95% of luminescent activity in the absence of ecotoxicants. The BE tested in this work was demonstrated to have a long shelf life (> 60 weeks) at -80°C without changes in the baseline level of bioluminescence. Copyright © 2016 John Wiley & Sons, Ltd.

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Bioluminescent Models to Evaluate the Efficiency of Light-Based Antibacterial Approaches

Gomes

Faustino

Neves

et al. 2022

Methods in Molecular Biology

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Bacterial bioluminescence inhibition by Chlorophenols

Cited by 7 publications

References 17 publications

Inhibitory Effects of Phenolic Ecotoxicants on Photobacteria at Various pH Values

Inhibitory Effects of Phenolic Ecotoxicants on Photobacteria at Various pH Values

Biosensitive element in the form of immobilized luminescent photobacteria for detecting ecotoxicants in aqueous flow‐through systems

Bioluminescent Models to Evaluate the Efficiency of Light-Based Antibacterial Approaches

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