Bacterial Bioluminescent Biosensor Characterisation for On-line Monitoring of Heavy Metals Pollutions in Waste Water Treatment Plant Effluents

Charrier, Thomas; Durand, Marie-José; Affi, Mahmoud; Jouanneau, Sulivan; Gezekel, Hélène; Thouand, Gérald

doi:10.5772/7210

Cited by 13 publications

(14 citation statements)

References 56 publications

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“…) . For instance, light emission signal is received by whole cell biosensor where, in the presence of gene‐inducing environmental pollutants, cells react with production of fluorescent proteins . Biosensor prototype based on modified bacteriophage carrying gene of fluorescent reporter protein was successfully tested for the detection of E. coli in sea water samples .…”

Section: Methods Of Detecting Microorganismsmentioning

confidence: 99%

Detection of Microbiological Content in Water as Indicator of Its Ecological State − Mini‐Review

Besarab

Новик

2016

CLEAN Soil Air Water

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Intense anthropogenic pressure on the native media causing grave ecological problems such as deficiency of drinking water, shift of natural evolution of ecosystems due to species extinction, emphasizes the need of accurate analytical systems for continuous environmental monitoring. Microorganisms as simple sensitive living units conducting nutrient metabolic cycles in the environment are applicable for proper assessment of ambient media state. The paper will review modes of microbial contribution in indication and will list important and standard micro-indicators of water pollution. The article will describe up-to-date direct methods of pollutant detection and indirect indicator-recognition techniques. The peculiarities and requirements of water quality monitoring correlated with high dynamics and mobility of aqueous media will be discussed.

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Section: Methods Of Detecting Microorganismsmentioning

confidence: 99%

Detection of Microbiological Content in Water as Indicator of Its Ecological State − Mini‐Review

Besarab

Новик

2016

CLEAN Soil Air Water

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“…In all cases, reporter genes lux CDABE are cloned downstream of a promoter allowing to highlight the specific or semi-specific presence for certain compounds in a sample [46,47]. In the case of metal detection, the promoters used are mostly involved in the mechanisms of bacterial resistance to heavy metals [48][49][50]. A set of five bioluminescent bacteria namely E. coli Taclux, E. coli Zntlux, E. coli Arslux, E. coli Coplux and E. coli Merlux was used in this study (Table 1).…”

Section: Induction Of Bioluminescent Bacteriamentioning

confidence: 99%

“…Detection capabilities of the ecotoxicity of the leachate are actually dependent on the test used [62,63] and it has been already shown that V. fischeri was generally less sensitive than D. magna [10,64]. Instead of these tests, experiments by using bacterial strains allowed to determine and quantify the element which was potentially bioavailable and/or toxic for bacteria [50]. Then, response in ecotoxicity tests was not always directly correlated with total or water-soluble concentrations [65].…”

Section: Relevance Of Ecotoxicity Tests For Risk Assessment Posed By mentioning

confidence: 99%

Use of ecotoxicity test and ecoscores to improve the management of polluted soils: case of a secondary lead smelter plant

Foucault

Durand

Tack

et al. 2013

Journal of Hazardous Materials

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OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible. This is an author-deposited version published in : http://oatao.univ-toulouse.fr/ Eprints ID : 11346 a b s t r a c tWith the rise of sustainable development, rehabilitation of brownfield sites located in urban areas has become a major concern. Management of contaminated soils in relation with environmental and sanitary risk concerns is therefore a strong aim needing the development of both useful tools for risk assessment and sustainable remediation techniques. For soils polluted by metals and metalloids (MTE), the criteria for landfilling are currently not based on ecotoxicological tests but on total MTE concentrations and leaching tests. In this study, the ecotoxicity of leachates from MTE polluted soils sampled from an industrial site recycling lead-acid batteries were evaluated by using both modified Escherichia coli strains with luminescence modulated by metals and normalized Daphnia magna and Alivibrio fischeri bioassays. The results were clearly related to the type of microorganisms (crustacean, different strains of bacteria) whose sensitivity varied. Ecotoxicity was also different according to sample location on the site, total concentrations and physico-chemical properties of each soil. For comparison, standard leaching tests were also performed. Potentially phytoavailable fraction of MTE in soils and physico-chemical measures were finally performed in order to highlight the mechanisms. The results demonstrated that the use of a panel of microorganisms is suitable for hazard classification of polluted soils. In addition, calculated eco-scores permit to rank the polluted soils according to their potentially of dangerousness. Influence of soil and MTE characteristics on MTE mobility and ecotoxicity was also highlighted.

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“…In addition to the ability to produce light to win in this competition, bioluminescence microorganism have the ability to protect hosts from pathogenic microorganisms. These properties allow the use of luminescent microbial isolates genes in the field of ecology as heavy metal biosensor agents in the aquatic environment [9]. Therefore, the potential of bioluminescence studies is very promising in the field of biotechnology and ecology.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Symbiont Microorganisms from Bioluminescent Marine Life

Pringgenies

Dewi

Apriliyani

2019

ARRB

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Bioluminescence means the ability of animals or plants to naturally produce light. The three known ways by which bioluminescence is produced are through specific cells called photocytes, bioluminescent glands in tissues and symbiotic bioluminescent microorganisms. Bioluminescence in Loligo duvaucelii is known to be caused by the presence of symbiotic microorganisms in bioluminescent sacs. There is a need to compile more information on bioluminescent symbiotic microorganisms on marine life in Indonesia and their potential. This study aims to determine the species of bioluminescent microorganisms on squid and fish, namely Loligo sp. and Loligo edulis from the waters of Jepara and the Bombay duck (Harpadon nehereus) from the Strait of Malacca, Indonesia and their potential. The samples were collected by isolating the microorganisms from the luminescent organs, after which the bioluminescent microorganisms were used in the research. This research consisted of antimicrobial tests against pathogenic microorganisms which were conducted qualitatively. The bioluminescent microorganisms were identified using biochemical assay and molecular assay (16S rRNA PCR). Tests results from Loligo sp. symbiotic microorganisms found two isolates which showed antimicrobial activities against pathogenic Multi Drug Resistant (MDR) microorganisms, namely uncultured bacterium clone 1P-1-G05 against Escherichia coli with 32.59 mm of inhibitory zone and Uncultured bacterium clone 3g10a against Enterobacter sp. with 28.44 mm of inhibitory zone. The bioluminescent symbiont microorganisms in Loligo edulis, which was identified to be Photobacterium phosphoreum, showed antimicrobial activities against Vibrio harveyi, E. coli, Staphylococcus aureus, and Bacillus sp. Bioluminescent symbiotic microorganisms on H. nehereus identified Alteromonas macleodii, which showed gamma hemolysis on the blood agar test.

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Bacterial Bioluminescent Biosensor Characterisation for On-line Monitoring of Heavy Metals Pollutions in Waste Water Treatment Plant Effluents

Cited by 13 publications

References 56 publications

Detection of Microbiological Content in Water as Indicator of Its Ecological State − Mini‐Review

Detection of Microbiological Content in Water as Indicator of Its Ecological State − Mini‐Review

Use of ecotoxicity test and ecoscores to improve the management of polluted soils: case of a secondary lead smelter plant

Isolation and Identification of Symbiont Microorganisms from Bioluminescent Marine Life

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