Bacterial capsular polysaccharides—biochemistry, immunity and vaccine

Cj, Lee

doi:10.1016/0161-5890(87)90067-8

Cited by 68 publications

(1 citation statement)

References 137 publications

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“…By nonspecific activation of hydroxyl groups and creation of reactive cyanate esters, cyanogen bromide has been employed to conjugate carbohydrates and protein amino groups in aqueous alkaline solution via a stable O -alkyl isourea linkage [ 76 ]. When a phosphate presents at the terminal end, it frequently appears in an internal ester, because of the closeness of the adjoining hydroxyl group on the ribitol moiety to the phosphate hydroxyl; this internal esterification triggers Hib PS hydrolytic lability at both alkaline and acid pH and at elevated temperature [ 77 ].…”

Section: Hib Vaccinesmentioning

confidence: 99%

Hib Vaccines: Past, Present, and Future Perspectives

Zarei

Almehdar

Redwan

2016

Journal of Immunology Research

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Haemophilus influenzae type b (Hib) causes many severe diseases, including epiglottitis, pneumonia, sepsis, and meningitis. In developed countries, the annual incidence of meningitis caused by bacteria is approximately 5–10 cases per population of 100,000. The Hib conjugate vaccine is considered protective and safe. Adjuvants, molecules that can enhance and/or regulate the fundamental immunogenicity of an antigen, comprise a wide range of diverse compounds. While earlier developments of adjuvants created effective products, there is still a need to create new generations, rationally designed based on recent discoveries in immunology, mainly in innate immunity. Many factors may play a role in the immunogenicity of Hib conjugate vaccines, such as the polysaccharides and proteins carrier used in vaccine construction, as well as the method of conjugation. A Hib conjugate vaccine has been constructed via chemical synthesis of a Hib saccharide antigen. Two models of carbohydrate-protein conjugate have been established, the single ended model (terminal amination-single method) and cross-linked lattice matrix (dual amination method). Increased knowledge in the fields of immunology, molecular biology, glycobiology, glycoimmunology, and the biology of infectious microorganisms has led to a dramatic increase in vaccine efficacy.

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Section: Hib Vaccinesmentioning

confidence: 99%

Hib Vaccines: Past, Present, and Future Perspectives

Zarei

Almehdar

Redwan

2016

Journal of Immunology Research

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Polyclonal B-Cell activation byNeisseria meningitidis capsular polysaccharides elicit antibodies protective againstTrypanosoma cruzi infection in vitro

Oliveira

Milani

Travassos

1996

J. Clin. Lab. Anal.

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CE to monitor endotoxins by protein complexation

et al. 2006

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A new CE method for fast and efficient analysis of bacterial endotoxins (lipopolysaccharides) is described. It is based on the strong interaction between proteins and endotoxins. The UV absorption of the protein component in the complex is used for the detection. The electrophoretic mobility of the complex hemoglobin/endotoxin can be employed for qualitative analysis of the endotoxin. For instance, the structural differences between "smooth" and "rough" lipopolysaccharides from Salmonella minnesota (wild-type), Salmonella minnesota R595 and Shigella sonnei R562H are reflected in the electrophoretic mobilities of their hemoglobin complex.

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Bacterial capsular polysaccharides—biochemistry, immunity and vaccine

Cited by 68 publications

References 137 publications

Hib Vaccines: Past, Present, and Future Perspectives

Hib Vaccines: Past, Present, and Future Perspectives

Polyclonal B-Cell activation byNeisseria meningitidis capsular polysaccharides elicit antibodies protective againstTrypanosoma cruzi infection in vitro

CE to monitor endotoxins by protein complexation

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