Bacterial Cinnamoyl Esterase Activity Screening for the Production of a Novel Functional Food Product

Abstract

Lactobacillus helveticus MIMLh5 was selected for its strong cinnamoyl esterase activity on chlorogenic acid and employed for the preparation of a food product containing a high concentration of free caffeic acid. The novel food product was demonstrated to display high total antioxidant power and potential probiotic properties.

Marine Microorganisms as Source of Stereoselective Esterases and Ketoreductases: Kinetic Resolution of a Prostaglandin Intermediate

“…Hydrolytic activity observed in whole cultures can be due to extracellular or cell-bound activity (Gandolfi et al 2000;Guglielmetti et al 2008). Thus, extracellular and cell-bound activities were measured independently, showing that esterase activity was mostly cell-bound.…”

Lactobacillus helveticus MIMLh5-Specific Antibodies for Detection of S-Layer Protein in Grana Padano Protected-Designation-of-Origin Cheese

“…helveticus MIMLh5 was isolated from natural whey starter (NWS) used in the production of Grana Padano PDO cheese (2). The dynamics of the bacterial population during Grana Padano PDO cheese production and ripening was monitored (38), and the persistence of L. helveticus strains in NWS and during early cheese ripening was demonstrated (39)(40)(41)(42)(43).…”

“…helveticus MIMLh5 is a dairy bacterial strain isolated from the natural whey starter of Grana Padano, a protected-designation-of-origin (PDO) cheese (2,3). MIMLh5 was included in previous studies for its metabolic, biotechnological, and probiotic features (2,4,5) and, interestingly, for its immunomodulatory properties (5)(6)(7). Particularly, L. helveticus MIMLh5 was proposed as a potential pharyngeal probiotic because of its ability to adhere to human epithelial cell lines and to efficiently antagonize group A streptococci on these cells (6).…”

Crystallization and preliminary X-ray analysis of a novel halotolerant feruloyl esterase identified from a soil metagenomic library

“…In the papermaking industry, raw plant materials could be treated with FAEs and other enzymes to increase the alkaline solubility of the product and to reduce the use of chlorine in the bleaching process (Record et al, 2003;Sigoillot et al, 2005). In the feed industry, FAEs could be used to improve feed efficiency (Guglielmetti et al, 2008). In bio-energy, FAEs could increase the yield of fermentable sugars such as glucose and xylose (Tabka et al, 2006).…”