Bacterial community associated with <i>Pfiesteria</i>‐like dinoflagellate cultures

Alavi, Mohammad; Miller, Todd R.; Erlandson, Karn; Schneider, Rachel; Belas, Robert

doi:10.1046/j.1462-2920.2001.00207.x

Cited by 148 publications

(176 citation statements)

References 39 publications

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“…Roseobacters have been isolated from seawater, marine sediments, surfaces of marine organisms, and hypersaline ponds (Buchan and Moran, 2005) and can constitute up to 2-8% of surface water bacterioplankton (Wietz et al, 2010;Sunagawa et al, 2015), but the clade can represent as much as 20-40% of sequence data from 16S ribosomal RNA (rRNA) gene libraries especially during algal blooms (Moran et al, 2003;Buchan and Moran, 2005;Prabagaran et al, 2007). Many of the Roseobacter-clade strains are associated with algae and can metabolize dimethylsulfoniopropionate produced by algae (González et al, 2000;Alavi et al, 2001;Miller and Belas, 2004;Buchan and Moran, 2005;Todd et al, 2009). Cosmopolitan distribution has been demonstrated for the SAR11 clade also belonging to the alphaproteobacteria (Wietz et al, 2010;Giebel et al, 2011;Ghiglione et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis

et al. 2016

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Tropodithietic acid (TDA)-producing Ruegeria mobilis strains of the Roseobacter clade have primarily been isolated from marine aquaculture and have probiotic potential due to inhibition of fish pathogens. We hypothesized that TDA producers with additional novel features are present in the oceanic environment. We isolated 42 TDA-producing R. mobilis strains during a global marine research cruise. While highly similar on the 16S ribosomal RNA gene level (99-100% identity), the strains separated into four sub-clusters in a multilocus sequence analysis. They were further differentiated to the strain level by average nucleotide identity using pairwise genome comparison. The four sub-clusters could not be associated with a specific environmental niche, however, correlated with the pattern of sub-typing using co-isolated phages, the number of prophages in the genomes and the distribution in ocean provinces. Major genomic differences within the sub-clusters include prophages and toxin-antitoxin systems. In general, the genome of R. mobilis revealed adaptation to a particle-associated life style and querying TARA ocean data confirmed that R. mobilis is more abundant in the particle-associated fraction than in the free-living fraction occurring in 40% and 6% of the samples, respectively. Our data and the TARA data, although lacking sufficient data from the polar regions, demonstrate that R. mobilis is a globally distributed marine bacterial species found primarily in the upper open oceans. It has preserved key phenotypic behaviors such as the production of TDA, but contains diverse sub-clusters, which could provide new capabilities for utilization in aquaculture.

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Section: Introductionmentioning

confidence: 99%

Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis

et al. 2016

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“…The taxonomic identifications of the bacteria in Pseudo-nitzschia cultures revealed members of the Roseobacter clade, Flavobacteria, and Gammaproteobacteria, all frequently found with other phytoplankton cultures (Stewart et al 1997, Alavi et al 2001, Hold et al 2001, Schäfer et al 2002, Kobayashi et al 2003, Fandino et al 2005, Grossart et al 2005, Jasti et al 2005, Kaczmarska et al 2005, Sapp et al 2007a. However, the level of intraspecific diversity was unexpected; 9 of 10 ARISA peaks sequenced in replicate contained multiple nonidentical 16S rDNA sequences, suggesting that multiple strains of the same bacterial species (> 99% 16S rDNA identity; Acinas et al 2004) coexisted in Pseudo-nitzschia cultures.…”

Section: Discussionmentioning

confidence: 99%

“…Some of these bacteria may occur intracellularly (Kobayashi et al 2003), and these same bacterial taxa often co-occur among other diatom and dino flagellate cultures (Alavi et al 2001, Hold et al 2001, Schäfer et al 2002, Fandino et al 2005, Grossart et al 2005, Jasti et al 2005, Sapp et al 2007a). For Pseudonitzschia, coexisting bacteria have been described only for 1 species, the typically toxigenic species P. multiseries.…”

Section: Introductionmentioning

confidence: 99%

Bacterial community composition differs with species and toxigenicity of the diatom Pseudo-nitzschia

Guannel¹,

Horner-Devine²,

Rocap

2011

Aquat. Microb. Ecol.

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Interactions between bacteria and members of the marine diatom genus Pseudonitzschia may enhance production of the toxin domoic acid (DA) by toxigenic strains of Pseudonitzschia. To gain a broader understanding of relationships between bacteria and Pseudo-nitzschia species, we used automated ribosomal intergenic spacer analysis (ARISA) to assess the composition of the bacterial communities coexisting with 18 Pseudo-nitzschia strains representing 6 species. For cultures surveyed across multiple time points and size fractions, the attached and free-living bacterial communities were not significantly distinct from one another, and bacterial composition was stable across diatom growth phases (exponential versus stationary) and approximately 1 yr in culture. Among all cultures, bacterial communities differed significantly with Pseudo-nitzschia species and toxigenicity. Toxigenic strains of Pseudo-nitzschia hosted fewer bacterial ARISA operational taxonomic units (OTUs), in comparison to nontoxigenic strains. We constructed two 16S rDNA clone libraries to identify bacteria coexisting with 1 P. multiseries (toxigenic) and 1 P. delicatissima (nontoxigenic) culture. Both cultures hosted members of the Roseobacter clade, Gammaproteobacteria, and Flavobacteria, yet the specific bacteria coexisting with each Pseudo-nitzschia strain differed at the genus level or above. Our findings support the hypothesis that bacterial communities respond to DA or other species-specific differences in the environments created by Pseudo-nitzschia strains.KEY WORDS: Pseudo-nitzschia · Domoic acid · Phytoplankton-bacteria interactions · ARISA Resale or republication not permitted without written consent of the publisher

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“…Bacteria within this clade are known to be ubiquitous and rapid colonizers of surfaces in coastal environments (Dang and Lovell, 2000) and have been enumerated as the most abundant group within the bacterial assemblages associated with some marine algal cultures and phytoplankton blooms in nature (Gonzalez et al, 2000;Riemann et al, 2000;Alavi et al, 2001). Given that many of the DGGE sequences fell within the Roseobacter clade, this group was selected for a detailed quantification on the surface of U. australis.…”

Section: ) and Coralsmentioning

confidence: 99%

Variability and abundance of the epiphytic bacterial community associated with a green marine Ulvacean alga

et al. 2009

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Marine Ulvacean algae are colonized by dense microbial communities predicted to have an important role in the development, defense and metabolic activities of the plant. Here we assess the diversity and seasonal dynamics of the bacterial community of the model alga Ulva australis to identify key groups within this epiphytic community. A total of 48 algal samples of U. australis that were collected as 12 individuals at 3 monthly intervals, were processed by applying denaturing gradient gel electrophoresis (DGGE), and three samples from each season were subjected to catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH). CARD-FISH revealed that the epiphytic microbial community was comprised mainly of bacterial cells (90%) and was dominated by the groups Alphaproteobacteria (70%) and Bacteroidetes (13%). A large portion (47%) of sequences from the Alphaproteobacteria fall within the Roseobacter clade throughout the different seasons, and an average relative proportion of 19% was observed using CARD-FISH. DGGE based spatial (between tidal pools) and temporal (between season) comparisons of bacterial community composition demonstrated that variation occurs. Between individuals from both the same and different tidal pools, the variation was highest during winter (30%) and between seasons a 40% variation was observed. The community also includes a sub-population of bacteria that is consistently present. Sequences from excised DGGE bands indicate that members of the Alphaproteobacteria and the Bacteroidetes are part of this stable sub-population, and are likely to have an important role in the function of this marine epiphytic microbial community.

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Bacterial community associated with Pfiesteria‐like dinoflagellate cultures

Cited by 148 publications

References 39 publications

Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis

Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis

Bacterial community composition differs with species and toxigenicity of the diatom Pseudo-nitzschia

Variability and abundance of the epiphytic bacterial community associated with a green marine Ulvacean alga

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