Bacterial community composition characterization of a lead-contaminated Microcoleus sp. consortium

Giloteaux, Ludovic; Solé, Antonio; Esteve, Isabel; Duran, Robert

doi:10.1007/s11356-010-0432-x

Cited by 8 publications

(1 citation statement)

References 43 publications

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“…Recent developments in molecular fingerprinting techniques, such as denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism, and real-time PCR quantification, have allowed researchers to investigate the microbial diversity variations in soils contaminated with heavy metals (Berg et al 2012;Cáliz et al 2013;Giloteaux et al 2011;Petrić et al 2011;Zhao et al 2014). Molecular fingerprint techniques have high resolution; however, the fingerprint complexity caused by the high richness of indigenous microbial communities in soil may mask the changes induced by heavy metals (Gomes et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas

Chen

Xing

et al. 2015

Environ Sci Pollut Res

196

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Mine activities leaked heavy metals into surrounding soil and may affected indigenous microbial communities. In the present study, the diversity and composition of the bacterial community in soil collected from three regions which have different pollution degree, heavy pollution, moderate pollution, and non-pollution, within the catchment of Chao River in Beijing City, were compared using the Illumina MiSeq sequencing technique. Rarefaction results showed that the polluted area had significant higher bacterial alpha diversity than those from unpolluted area. Principal component analysis (PCA) showed that microbial communities in the polluted areas had significant differences compared with the unpolluted area. Moreover, PCA at phylum level and Matastats results demonstrated that communities in locations shared similar phyla diversity, indicating that the bacterial community changes under metal pollution were not reflected on phyla structure. At genus level, the relative abundance of dominant genera changed in sites with degrees of pollution. Genera Bradyrhizobium, Rhodanobacter, Reyranella, and Rhizomicrobium significantly decreased with increasing pollution degree, and their dominance decreased, whereas several genera (e.g., Steroidobacter, Massilia, Arthrobacter, Flavisolibacter, and Roseiflexus) increased and became new dominant genera in the heavily metal-polluted area. The potential resistant bacteria, found within the genera of Thiobacillus, Pseudomonas, Arthrobacter, Microcoleus, Leptolyngbya, and Rhodobacter, are less than 2.0 % in the indigenous bacterial communities, which play an important role in soil ecosystem. This effort to profile the background diversity may set the first stage for better understanding the mechanism underlying the community structure changes under in situ mild heavy metal pollution.

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Section: Introductionmentioning

confidence: 99%

Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas

Chen

Xing

et al. 2015

Environ Sci Pollut Res

196

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Microbiology of Healing Mud (Fango) from Roman Thermae Aquae Iasae Archaeological Site (Varaždinske Toplice, Croatia)

et al. 2014

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We found well-preserved, rocky artefacts that had been buried in the healing mud (fango) for more than 1,500 years at the Roman archaeological site at Varaždinske Toplice. This Roman pool with fango sediments and artefacts is fed from hot sulphidic springs. The fango exhibited nearly neutral pH, a high level of organic C, an elevated concentration of heavy metals and a high total microbial biomass, greater than 10(8) cells per gram of dry weight. The dominant microbes, assessed by molecular profiling (denaturing gradient gel electrophoresis), were affiliated with Thiobacillus, Sulfuricurvum, Polaromonas, and Bdellovibrio. Polymerase chain reaction screening for microbial functional guilds revealed the presence of sulphur oxidizers and methanogens but no sulphate reducers. The dominance of four Proteobacterial classes (α-, β-, δ- and ε-Proteobacteria) was confirmed by fluorescence in situ hybridisation; Actinobacteria were less abundant. Cultivable bacteria represented up to 23.4 % of the total bacterial counts when cultivation media was enriched with fango. These bacteria represented the genera Acinetobacter, Aeromonas, Arthrobacter, Comamonas, Ewingella, Flavobacterium, Pseudomonas, Rahnella and Staphylococcus. This study showed that the heterogeneous nature of fango at neutral pH created various microniches, which largely supported microbial life based on sulphur-driven, autotrophic denitrification.

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Interactions between Zn and bacteria in marine tropical coastal sediments

Pringault

Viret

Duran

2011

Environ Sci Pollut Res

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In the three studied sediments, Zn spiking resulted in only slight changes in bacterial community structure. In contrast, the addition of low concentrations of glucose (5 mM) strongly modified the bacterial community structure: <20% of similarity with the initial structure concomitant with a strong diminution of the specific richness. Overall, these results suggest that highly labile organic matter has a larger impact on microbial structure than heavy metal. These weak impacts of Zn on bacteria diversity might be partly explained by (1) the strong adsorption of Zn in the presence of bacteria and/or (2) the incorporation of Zn into a nonbioavailable fraction. Nevertheless, Zn spiking resulted in significant changes in nutrient cycles, suggesting that bacterial metabolisms were impacted by the heavy metal. This led to an increase in nutrient supplies to the water column, potentially enhancing eutrophication in a nutrient-limited, oligotrophic ecosystem.

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Bacterial community composition characterization of a lead-contaminated Microcoleus sp. consortium

Cited by 8 publications

References 43 publications

Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas

Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas

Microbiology of Healing Mud (Fango) from Roman Thermae Aquae Iasae Archaeological Site (Varaždinske Toplice, Croatia)

Interactions between Zn and bacteria in marine tropical coastal sediments

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