Bacterial community structure and detection of putative plant growth-promoting rhizobacteria associated with plants grown in Chilean agro-ecosystems and undisturbed ecosystems

Jorquera, Milko A.; Inostroza, Nitza G.; Lagos, Lorena M.; Barra, Patricio Javier; Marileo, Luis; Rilling, Joaquín I.; Campos, Daniela Cavalcante dos Santos; Crowley, David E.; Richardson, Alan; Mora, Marı́a de la Luz

doi:10.1007/s00374-014-0935-6

Cited by 41 publications

(43 citation statements)

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“…The bacterial diversity and the ecology of the other phytase classes remain poorly characterized (Mullaney and Ullah 2003;Hill and Richardson 2006;Lim et al 2007;Huang et al 2011). The BPP class of phytase enzymes were first isolated from Bacillus strains (Kerovuo et al 1998;Shin et al 2001), and have since been shown to occur in a wide range of environments (Cheng and Lim 2006;Greiner et al 2007;Lim et al 2007;Huang et al 2009;Jorquera et al 2012Jorquera et al , 2014. Phylogenetic analysis of BPP genes in a range of bacteria, mostly from aquatic environments Lim et al 2007;Huang et al 2009), revealed suitable DNA homology for the design of BPPspecific probes (Huang et al 2009).…”

Section: Introductionmentioning

confidence: 98%

“…However, a variety of microorganisms have been shown to hydrolyse phytate in various ecosystems (Richardson and Hadobas 1997;Yanke et al 1998;Idriss et al 2002;Hill and Richardson 2006;Hill et al 2007;Nakashima et al 2007;Jorquera et al 2008a;Huang et al 2009). Many organisms harbour a phytase gene in their genomes Jorquera et al 2008bJorquera et al , 2012Jorquera et al , 2014, though the catalytic efficiency of these phytase homologues has not yet been demonstrated biochemically. Pseudomonas and Bacillus strains have been isolated from soils and studied for their ability to hydrolyse phytate and to enhance the P nutrition of plants (Richardson and Hadobas 1997;Richardson et al 2001b;Idriss et al 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Phylogenetic analysis of BPP genes in a range of bacteria, mostly from aquatic environments Lim et al 2007;Huang et al 2009), revealed suitable DNA homology for the design of BPPspecific probes (Huang et al 2009). BPP genes previously characterized from rhizosphere are mainly affiliated to Firmicutes (Jorquera et al 2011(Jorquera et al , 2012(Jorquera et al , 2014.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of functional diversity and structure of phytate-hydrolysing bacterial community in Lolium perenne rhizosphere

2015

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Background and aims Plant growth is frequently limited by the availability of inorganic phosphorus (P) in the soil. In most soils, a considerable amount of the soil P is bound to organic molecules. Of these, phytate is the most abundant identifiable organic P form, but is not readily available to plants. In contrast, microorganisms have been shown to degrade phytate with high efficiency. The current study aims to characterize the members of the phytate-hydrolysing bacterial community in rhizosphere, and the molecular and enzymatic ability of these bacteria to degrade phytate. Methods and resultsThe phytate-hydrolysing bacterial community was characterized from the rhizosphere of plants cultivated in the presence or absence of phytate supplementation. Major changes in the bacterial community structure were observed with both culturedependent and -independent methods, which highlighted the predominance of Proteobacteria and Actinobacteria. Phytase activity was detected for a range of rhizobacterial isolates as well as the presence of, β-propeller phytases (BPP) for both isolates and directly in a soil sample. Conclusion A wide taxonomic range of functional phytate utilizers have been discovered, in soil bacterial taxa that were previously not well known for their ability to utilise phytate as P or C sources. This study provides new insights into microbial carbon and phosphorus cycling in soil.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of functional diversity and structure of phytate-hydrolysing bacterial community in Lolium perenne rhizosphere

2015

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“…Biogeography, plant species, and physicochemical soil properties can influence the composition of rhizosphere microbial communities (Edwards et al 2006;Fierer and Jackson 2006;Stegen et al 2009), including those of plants grown in hostile environments (Ciccazzo et al 2014;Massaccesi et al 2015). Differences in bacterial community composition were also observed in rhizosphere soils of plants from agroecosystems and extreme environments in Chile, such as Atacama Desert (Jorquera et al 2013(Jorquera et al , 2014Neilson et al 2012). Bacterial diversity of rhizosphere soils from Atacama Desert differed from those of the other studied rhizosphere soils.…”

Section: Discussionmentioning

confidence: 54%

“…per gram soil with the lowest values observed in the rhizosphere soils of Atacama Desert. This is a hyperarid ecosystem where low numbers of bacteria (from 1 × 10 2 to 3 × 10 5 CFU g −1 soil) and low amount of extractable DNA (<1.2 μg DNA g −1 of soil) from soil have been reported (Azua-Bustos et al 2015;Jorquera et al 2014;Maier et al 2004). However, Fletcher et al (2011) observed similar Fig.…”

Section: Discussionmentioning

confidence: 96%

Bacterial alkaline phosphomonoesterase in the rhizospheres of plants grown in Chilean extreme environments

et al. 2016

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Bacterial alkaline phosphomonoesterases (APases) are relevant for organic phosphorus (Po) recycling in many soils. However, the abundance and diversity of bacterial APase in the rhizospheres of native plants are poorly known, particularly in extreme environments. In this research work, we studied the composition of total and APase-harboring bacterial communities, abundances of selected APase genes (phoD and phoX), and APase activities in rhizosphere soils from native plants grown in extreme environments of northern (Atacama Desert), central (Andes volcano; Quetrupillan and Mamuil Malal) and hot spring (Liquiñe), and southern polar (Patagonia and Antarctic) regions of Chile. Differences in the composition of bacterial communities in the rhizosphere soils were revealed by denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR) of 16S ribosomal RNA (rRNA), phoD, and phoX genes. In general, the significant lowest bacterial diversities, APase gene abundances, and APase activities were observed in rhizosphere soils from Atacama Desert, whereas the highest values were observed in rhizosphere soils of Patagonia. In addition, APase gene abundances were positively correlated among them and with APase activity of rhizosphere soils, but negatively correlated with phosphorus (P) availability in rhizosphere soils. Although bacterial APases were observed in all studied rhizosphere soils, their relevance to soil Po recycling in soils of extreme environments remains unclear and further studies are needed.

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Rhizobacterial Community Structures Associated with Native Plants Grown in Chilean Extreme Environments

et al. 2016

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Chile is topographically and climatically diverse, with a wide array of diverse undisturbed ecosystems that include native plants that are highly adapted to local conditions. However, our understanding of the diversity, activity, and role of rhizobacteria associated with natural vegetation in undisturbed Chilean extreme ecosystems is very poor. In the present study, the combination of denaturing gradient gel electrophoresis and 454-pyrosequencing approaches was used to describe the rhizobacterial community structures of native plants grown in three representative Chilean extreme environments: Atacama Desert (ATA), Andes Mountains (AND), and Antarctic (ANT). Both molecular approaches revealed the presence of Proteobacteria, Bacteroidetes, and Actinobacteria as the dominant phyla in the rhizospheres of native plants. Lower numbers of operational taxonomic units (OTUs) were observed in rhizosphere soils from ATA compared with AND and ANT. Both approaches also showed differences in rhizobacterial community structures between extreme environments and between plant species. The differences among plant species grown in the same environment were attributed to the higher relative abundance of classes Gammaproteobacteria and Alphaproteobacteria. However, further studies are needed to determine which environmental factors regulate the structures of rhizobacterial communities, and how (or if) specific bacterial groups may contribute to the growth and survival of native plants in each Chilean extreme environments.

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Bacterial community structure and detection of putative plant growth-promoting rhizobacteria associated with plants grown in Chilean agro-ecosystems and undisturbed ecosystems

Cited by 41 publications

References 58 publications

Assessment of functional diversity and structure of phytate-hydrolysing bacterial community in Lolium perenne rhizosphere

Assessment of functional diversity and structure of phytate-hydrolysing bacterial community in Lolium perenne rhizosphere

Bacterial alkaline phosphomonoesterase in the rhizospheres of plants grown in Chilean extreme environments

Rhizobacterial Community Structures Associated with Native Plants Grown in Chilean Extreme Environments

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