Bacterial community structure and function in a metal‐working fluid

Gast, Christopher J. van der; Whiteley, Andrew S.; Lilley, Andrew K.; Knowles, Christopher J.; Thompson, Ian P.

doi:10.1046/j.1462-2920.2003.00428.x

Cited by 86 publications

(45 citation statements)

References 25 publications

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“…Similarities analysis was conducted using the unweighted-pair group method with the arithmetic average (UPGMA) clustering algorithm in Quantity One software (24). All data analyses were performed using the statistical package for the social sciences (SPSS) software v.20.0 (SPSS, Inc., Chicago, IL, USA).…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Fecal Microbiota in 5 - 8-Year-old Children with and without Cow Milk Protein Allergy

et al. 2016

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Background: The bacterial diversity and microbial communities in the feces of children with cow milk protein allergy (CMPA) may be altered; these changes have not been clearly elucidated in 5 -8-year-old children with CMPA. Objectives: The aim of this study was to compare the bacterial diversity and microbial communities of feces in 5 -8-year-old children with CMPA and those of healthy children of the same age. Methods: Stool samples from 12 children with CMPA and 12 healthy individuals were collected. Total bacterial DNA of all samples were extracted and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) using bacterial v3 universal primers and the species-specific primers of Bifidobacterium, Lactobacillus, and Clostridium coccoides. The preponderant bacterial bands were identified by gene sequencing. Results:The results revealed that the diversity of fecal dominant microbiota and C. coccoides group in children with CMPA were significantly higher (P < 0.05) than in healthy children. Meanwhile, a lower degree of diversity in the Bifidobacterium group and no difference in the diversity of the Lactobacillus group were found in the feces of children with CMPA. Bacteroides, Clostridium, and Escherichia coli were more abundant in the fecal microbiota of CMPA children than in healthy controls. In addition, C. celerecrescens was found only in the fecal microbiota of CMPA children, whereas B. bifidum was found only in the feces of healthy children. Conclusions: There were some notable changes in the fecal microbiota of 5 -8-year-old children with CMPA.

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Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Fecal Microbiota in 5 - 8-Year-old Children with and without Cow Milk Protein Allergy

et al. 2016

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“…The so-called hybrid processes make use of more than one separation techniques that complement one another and they may be supported by biological treatment stage [1,9]. Such integrated processes take the advantage of the novel technological as well as biotechnological achievements to reduce total treatment costs, energy consumption, and finally to give the most efficient result [1,9,10,30,32,34,40,41,43,48].…”

Section: Discussionmentioning

confidence: 99%

“…Microbiologically-based treatment processes make use of the activities of activated sludges working in aeration chambers of wastewater treatment plants or of bacterial consortia constructed upon specialized strains [9,22,34,35,39,40,42]. Bioremediation of recalcitrant MWF constituents was shown to be effective either with aerobic or anaerobic methods [9,14,39,49].…”

Section: Discussionmentioning

confidence: 99%

“…The first strategy involves either biostimulation of the indigenous microflora within the treated MWF [9,39] or selection of the most active strains from various MWF samples, then cultivation and inoculation to a new wastewater to be treated [9,30,34,35,[40][41][42]. The use of indigenous microorganisms is of a special interest since their resistance to a complex chemical content of MWFs might result in elevated xenobiotic biodegradation activity [13,14,40,43].…”

Section: Introductionmentioning

confidence: 99%

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Bioremediation Of A Spent Metalworking Fluid With Auto- And Allochthonous Bacterial Consortia

Kaszycki

Petryszak

Supel

2015

Ecological Chemistry and Engineering S

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Spent mineral oil-based metalworking fluids are waste products of the machining processes and contribute substantially to the global industrial pollution with petroleum oil products. Wastewaters containing oily emulsions are ecologically hazardous and thus a variety of methods have been implemented to prevent these effluents from affecting the natural environment. Most of these methods rely upon physical-chemical treatment and phase separation; however, none of them proved to be effective enough to meet tightening environmental regulations. Therefore, novel technologies need to be elaborated and there is growing interest in implementing biological treatment methods based on microbial bioremediation. In this study an oil/water emulsion obtained from a waste stream of the metal-processing industry was tested for biodegradability of its organic constituents. This liquid waste was found non-toxic to bacterial consortia and was colonized with indigenous microorganisms (approx. 10 7 cfu · cm -3). The total load of organic content was determined as a chemical oxygen demand (COD) value of 48 200 mg O2 · dm -3. Emulsion treatment was carried out using a threefold wastewater dilution and employing two variants of biostimulated aerobic bacterial communities: (1) uninoculated emulsion, where bioremediation was carried out by the autochthonous bacteria alone, and (2) wastewater samples inoculated with a ZB-01 microbial consortium which served as a source of specialized bacteria for process bioaugmentation. Biodegradation efficiency achieved in a 14-day test was monitored by measuring both the COD parameter and the concentration of high-boiling organic compounds. Both approaches yielded satisfactory results showing significant reduction of the emulsion organic fraction; however, the resultant decrease of wastewater load tended to be more efficient for the case where the process was bioaugmented with the inoculated consortium. Gas chromatography analyses coupled with mass spectrometric detection (GC-MS) confirmed high degradation yields obtained for both cases studied (58 and 71%, respectively) in a 28-day test. It is concluded that oil-based metalworking emulsions can undergo efficient biological treatment under conditions enabling aerobic bacterial proliferation and that xenobiotic biodegradation kinetics can be accelerated by bioaugmenting the process with allochthonous microbial consortia.

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“…Molecular techniques can lead to a better understanding of overall microbial community in MWF (Wand et al, 1995;Tiedje and Stein, 1999;van der Gast, 2003). Recent research has applied culture-independent DNA based methods to identify specific 85 organisms of interest missed through their inability to grow under laboratory conditions.…”

Section: Background and Aimsmentioning

confidence: 99%

Biological and chemical hazards in water-mix metalworking fluids and mists

Brookes¹

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Bacterial community structure and function in a metal‐working fluid

Cited by 86 publications

References 25 publications

Comparative Analysis of Fecal Microbiota in 5 - 8-Year-old Children with and without Cow Milk Protein Allergy

Comparative Analysis of Fecal Microbiota in 5 - 8-Year-old Children with and without Cow Milk Protein Allergy

Bioremediation Of A Spent Metalworking Fluid With Auto- And Allochthonous Bacterial Consortia

Biological and chemical hazards in water-mix metalworking fluids and mists

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