Bacterial Community with Plant Growth-Promoting Potential Associated to Pioneer Plants from an Active Mexican Volcanic Complex

Rincón-Molina, Clara Ivette; Martı́nez-Romero, Esperanza; Aguirre-Noyola, José Luis; Manzano-Gómez, Luis Alberto; Zenteno-Rojas, Adalberto; Rogel, Marco A.; Rincón-Molina, Francisco Alexander; Rincón-Rosales, Reiner

doi:10.3390/microorganisms10081568

Cited by 10 publications

(9 citation statements)

References 119 publications

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“…These findings highlight the potential of native Rhizobium strains as biofertilizers to promote plant growth and health, and provide insights into the complex interactions between plant-associated bacteria and their environment. In this study, the bacterial communities associated with tomato plants inoculated with Rhizobium strains were found to be dominated by Proteobacteria, Acidobacteria, Bacteroidetes, Verrucomicrobia, and Gemmatimonadetes, which are commonly observed in the root microbiome of different plant species (Sivakumar et al, 2020;Rincón-Molina et al, 2022). Rhizobium belongs to the alpha-Proteobacteria group and is known for its multifunctional plant growth-promoting abilities, such as nitrogen fixation, phosphate solubilization, and auxin production.…”

Section: Treatmentmentioning

confidence: 83%

Enhance of tomato production and induction of changes on the organic profile mediated by Rhizobium biofortification

Gen-Jiménez,

Flores-Félix,

Rincón-Molina

et al. 2023

Front. Microbiol.

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IntroductionThe extensive use of chemical fertilizers has served as a response to the increasing need for crop production in recent decades. While it addresses the demand for food, it has resulted in a decline in crop productivity and a heightened negative environmental impact. In contrast, plant probiotic bacteria (PPB) offer a promising alternative to mitigate the negative consequences of chemical fertilizers. PPB can enhance nutrient availability, promote plant growth, and improve nutrient uptake efficiency, thereby reducing the reliance on chemical fertilizers.MethodsThis study aimed to evaluate the impact of native Rhizobium strains, specifically Rhizobium calliandrae LBP2-1, Rhizobium mayense NSJP1-1, and Rhizobium jaguaris SJP1- 2, on the growth, quality, and rhizobacterial community of tomato crops. Various mechanisms promoting plant growth were investigated, including phosphate solubilization, siderophore production, indole acetic acid synthesis, and cellulose and cellulase production. Additionally, the study involved the assessment of biofilm formation and root colonization by GFP-tagged strains, conducted a microcosm experiment, and analyzed the microbial community using metagenomics of rhizospheric soil.ResultsThe results showed that the rhizobial strains LBP2-1, NSJP1-1 and SJP1-2 had the ability to solubilize dicalcium phosphate, produce siderophores, synthesize indole acetic acid, cellulose production, biofilm production, and root colonization. Inoculation of tomato plants with native Rhizobium strains influenced growth, fruit quality, and plant microbiome composition. Metagenomic analysis showed increased Proteobacteria abundance and altered alpha diversity indices, indicating changes in rhizospheric bacterial community.DiscussionOur findings demonstrate the potential that native Rhizobium strains have to be used as a plant probiotic in agricultural crops for the generation of safe food and high nutritional value.

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Section: Treatmentmentioning

confidence: 83%

Enhance of tomato production and induction of changes on the organic profile mediated by Rhizobium biofortification

Gen-Jiménez,

Flores-Félix,

Rincón-Molina

et al. 2023

Front. Microbiol.

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“…We cultured the purified colony of S. yanoikuyae in 1 L of LB liquid medium at 25 • C for 24-36 h. We then measured the bacterial suspension with a spectrophotometer at OD600 to ensure that this value was at 1 (2 × 10 9 CFU/mL = 1.0) [36]. We determined the five different CFU concentration levels through the process of serial dilution.…”

Section: Bacterial Concentrationmentioning

confidence: 99%

“…Hence, the researchers considered it to function as PGPR. Rincón-Molina et al [36] inoculated pepper (Capsicum chinense) with S. yanoikuyae NFB69 and observed substantial increases in the crop's plant height, root length, and stem diameter. Furthermore, the bacteria greatly improved the crop's biomass and increased the number of fruits produced.…”

Section: Introductionmentioning

confidence: 99%

Effects of Sphingobium yanoikuyae SJTF8 on Rice (Oryza sativa) Seed Germination and Root Development

et al. 2022

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The interaction between plant roots and rhizobacterium communities plays a crucial role in sustainable agriculture. We aimed to assess the effects of Sphingobium yanoikuyae SJTF8 on rice seed germination and development, as well as to observe the effects of different concentrations of S. yanoikuyae SJTF8 on the root systems of rice seedlings. The bacteria are best known for their role in the bioremediation and biodegradation of pollutants, and thus far, there is research that supports their agricultural prospects. The experiment comprised five different S. yanoikuyae SJTF8 concentrations: SP-y 8 (108 CFU/mL); SP-y 7 (107 CFU/mL); SP-y 6 (106 CFU/mL); SP-y 5 (105 CFU/mL); SP-y 4 (104 CFU/mL). We used sterilized water as the control treatment. The bacteria triggered the synthesis of IAA, while the seedling root lengths substantially increased on the 12th day after germination. The high application concentrations of S. yanoikuyae SJTF8 resulted in higher IAA production (with the SP-y 7 and SP-y 8 concentrations ranging from 151,029 pg/mL to 168,033 pg/mL). We found that the appropriate concentrations of S. yanoikuyae SJTF8 when applied as an inoculant were SP-y 7 and SP-y 6, based on the increased root growth and biomass production. The bacteria were also able to solubilize phosphorous. The growth response from the rice seedlings when inoculated with S. yanoikuyae SJTF8 presents the potential of the bacteria as a growth promotor. Its application in rice cultivation could be a sustainable approach to rice production.

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“…In field-level biofertilization experiments, these bacterial strains have demonstrated a positive impact on growth and fruit production in crops, as well as in other non-leguminous plant species [ 11 ]. These native bacteria exhibit desirable characteristics as plant growth-promoting (PGP) bacteria, including a high nitrogen fixation potential, phosphate solubilization, auxin synthesis, and siderophore production [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Cost-Effective Cultivation of Native PGPB Sinorhizobium Strains in a Homemade Bioreactor for Enhanced Plant Growth

Manzano-Gómez,

Rincón-Rosales,

Flores-Felix

et al. 2023

Bioengineering

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The implementation of bioreactor systems for the production of bacterial inoculants as biofertilizers has become very important in recent decades. However, it is essential to know the bacterial growth optimal conditions to optimize the production and efficiency of bioinoculants. The aim of this work was to identify the best nutriment and mixing conditions to improve the specific cell growth rates (µ) of two PGPB (plant growth-promoting bacteria) rhizobial strains at the bioreactor level. For this purpose, the strains Sinorhizobium mexicanum ITTG-R7T and Sinorhizobium chiapanecum ITTG-S70T were previously reactivated in a PY-Ca2+ (peptone casein, yeast extract, and calcium) culture medium. Afterward, a master cell bank (MCB) was made in order to maintain the viability and quality of the strains. The kinetic characterization of each bacterial strain was carried out in s shaken flask. Then, the effect of the carbon and nitrogen sources and mechanical agitation was evaluated through a factorial design and response surface methodology (RSM) for cell growth optimization, where µ was considered a response variable. The efficiency of biomass production was determined in a homemade bioreactor, taking into account the optimal conditions obtained during the experiment conducted at the shaken flask stage. In order to evaluate the biological quality of the product obtained in the bioreactor, the bacterial strains were inoculated in common bean (Phaseolus vulgaris var. Jamapa) plants under bioclimatic chamber conditions. The maximum cell growth rate in both PGPB strains was obtained using a Y-Ca2+ (yeast extract and calcium) medium and stirred at 200 and 300 rpm. Under these growth conditions, the Sinorhizobium strains exhibited a high nitrogen-fixing capacity, which had a significant (p < 0.05) impact on the growth of the test plants. The bioreactor system was found to be an efficient alternative for the large-scale production of PGPB rhizobial bacteria, which are intended for use as biofertilizers in agriculture.

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Bacterial Community with Plant Growth-Promoting Potential Associated to Pioneer Plants from an Active Mexican Volcanic Complex

Cited by 10 publications

References 119 publications

Enhance of tomato production and induction of changes on the organic profile mediated by Rhizobium biofortification

Enhance of tomato production and induction of changes on the organic profile mediated by Rhizobium biofortification

Effects of Sphingobium yanoikuyae SJTF8 on Rice (Oryza sativa) Seed Germination and Root Development

Cost-Effective Cultivation of Native PGPB Sinorhizobium Strains in a Homemade Bioreactor for Enhanced Plant Growth

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