Bacterial composition of commercial probiotic products as evaluated by PCR-DGGE analysis

Fasoli, S.; Marzotto, Marta; Rizzotti, Lucia; Rossi, Franca; Dellaglio, Franco; Torriani, Sandra

doi:10.1016/s0168-1605(02)00259-3

Cited by 194 publications

(140 citation statements)

References 35 publications

Supporting

Mentioning

131

Contrasting

Unclassified

Order By: Relevance

“…To date, various molecular fingerprinting techniques, using different genetic markers, have proven useful in subspecies discrimination or strain differentiation. A number of studies evaluated the bacterial composition of commercial probiotic products for human consumption (Fasoli et al, 2003;Temmerman et al, 2003;Huys et al, 2006). Despite the availability of various molecular techniques, reports on the evaluation of the composition of probiotic products in China are rare.…”

Section: Molecular Techniques For Evaluation Of Commercial Probioticsmentioning

confidence: 99%

Probiotics in aquaculture of China — Current state, problems and prospect

et al. 2009

View full text Add to dashboard Cite

Section: Molecular Techniques For Evaluation Of Commercial Probioticsmentioning

confidence: 99%

Probiotics in aquaculture of China — Current state, problems and prospect

et al. 2009

View full text Add to dashboard Cite

“…Traditional microbiological methods for studying microbial communities, i.e. counts on differential and selective media followed by identification of isolates, are time consuming and highlight the disadvantages of low selectivity and reciprocal inhibition in plate (Fasoli et al 2003). Only one ISO standard exists for enumerating bifidobacteria in food.…”

Section: Resultsmentioning

confidence: 99%

“…Probiotic products should contain viable, defined microorganisms in sufficient numbers. Moreover, correct identification of the bacterial species used and agreement between the species declared on the label and those actually present in the product are both necessary for a probiotic product to be considered reliable (Fasoli et al 2003;Temmerman et al 2003). As stated above, there are still no official or generally accepted ISO standards for analysing the composition of bacterial species in probiotic products in Europe.…”

mentioning

confidence: 99%

Direct identification of bifidobacteria from probiotic supplements

Bunešová¹,

Vlková²,

Rada³

et al. 2014

Czech J. Food Sci.

View full text Add to dashboard Cite

Bunešová V., Vlková E., Rada V., Hovorková P., Musilová Š., Kmeť V. (2014): Direct identification of bifidobacteria from probiotic supplements. Czech J. Food Sci., 32: 132-136.The DNA of 14 probiotic supplements was isolated directly from various products without previous cultivation. The bifidobacteria composition declared by the manufacturer was determined by species-specific PCR. Such species found were in accordance with those listed on the products. This approach can be performed in less than 5 h and is applicable to other genera of probiotic bacteria. Bifidobacteria were also detected by culture-dependent analysis and MALDI-TOF MS. These methods drew the same results; however, they are more expensive and time consuming. So, we propose direct identification of bifidobacteria for routine quality control inspections of species composition in probiotic supplements.

show abstract

“…Attention to such features is important, as it has been repeatedly shown that in about 50% of the probiotic products the identity of recovered microorganisms does not correspond to the information stated on the product label (Yeung et al, 2002;Fasoli et al, 2003;Temmerman et al, 2003;Theunissen et al, 2005).…”

Section: Taxonomymentioning

confidence: 99%

“…Mislabelling may have serious safety implications. For example, mislabelling closely related species of lactobacilli maybe is not too worrying, but failure to properly identify or label the bacterial genus such as products labelling Lactobacillus acidophilus and containing Streptococcus sanguis (Yeung et al, 2002) or failure to list bacterial contents such as Enterococcus (Yeung et al, 2002) or Staphylococcus (Fasoli et al, 2003) are concerning from the point of view of safety of those products. These observations indicate the importance of rigorous quality control at every stage of the production and of each production batch.…”

Section: Taxonomymentioning

confidence: 99%

Assuring the continued safety of lactic acid bacteria used as probiotics

Gueimonde

Frías

Ouwehand³

2006

Biologia

View full text Add to dashboard Cite

Lactic acid bacteria have long been used to improve the safety of foods through fermentation. Some fermented products were also early used for their perceived health benefits, which lead to the development of probiotics as we now know them. Probiotics mainly belong to the genera Lactobacillus and Bifidobacterium. Most members of these genera are not considered pathogens or even opportunistic pathogens. Nevertheless, rare cases of Lactobacillus and Bifidobacterium infection have been reported, possibly even associated with the consumption of probiotic products. Such cases are extremely rare and the subjects always had severe underlying conditions most often affecting the immune system. There does not seem to be any risk for the general population. Safety assessments can be performed and many possible tests exist. It is, however, not certain these tests will prevent rare case of Lactobacillus infection in certain high-risk patients. The benefits of probiotic use should be weighed against the possible small risk. Such an evaluation will, in most cases, be favourable and should therefore not discourage consumption of probiotics.

show abstract

Bacterial composition of commercial probiotic products as evaluated by PCR-DGGE analysis

Cited by 194 publications

References 35 publications

Probiotics in aquaculture of China — Current state, problems and prospect

Probiotics in aquaculture of China — Current state, problems and prospect

Direct identification of bifidobacteria from probiotic supplements

Assuring the continued safety of lactic acid bacteria used as probiotics

Contact Info

Product

Resources

About