Bacterial conjugation within к-carrageenan gel beads: Biotic and abiotic factors affecting plasmid transfer

Mater, Denis D.G.; Craynest, M.; Barbotin, Jean‐Noël; Truffaut, Nicole; Thomas, Daniel

doi:10.1016/s0921-0423(96)80061-x

Cited by 1 publication

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Second, the population of cyst-like DF was heterogeneous and included DF with multilayered or single-layer thickened envelopes (Figures 6 and 8), as has been previously reported for DF of non-spore-forming bacteria of various taxa [18,47]. Third, the massive formation of pili (presumably type F) in the samples of SHG-immobilized HOB cells, which was more pronounced in the cases of A. seifertii and P. extremoaustralis, supports and confirms the previously shown higher ability of immobilized bacterial cells (compared to planktonic cultures) to carry out the exchange of genetic material [28,29]. Cell immobilization in SHG promotes the formation of fertile pili and provides for the preservation of their intact, undamaged state.…”

Section: Discussionsupporting

confidence: 86%

“…These were probably F-pili, responsible for the horizontal transfer of genetic material during transformation or transduction. The enhanced horizontal transfer of genetic determinants has been known to occur in the populations of immobilized cells [ 28 , 29 ], although we are not aware of reports on the visualization of the cell structures responsible for this process.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Ways of Long-Term Survival of Hydrocarbon-Oxidizing Bacteria in a New Biocomposite Material—Silanol-Humate Gel

et al. 2023

View full text Add to dashboard Cite

Immobilized bacterial cells are presently widely used in the development of bacterial preparations for the bioremediation of contaminated environmental objects. Oil hydrocarbons are among the most abundant pollutants. We have previously described a new biocomposite material containing hydrocarbon-oxidizing bacteria (HOB) embedded in silanol-humate gels (SHG) based on humates and aminopropyltriethoxysilane (APTES); high viable cell titer was maintained in this material for at least 12 months. The goal of the work was to describe the ways of long-term HOB survival in SHG and the relevant morphotypes using the techniques of microbiology, instrumental analytical chemistry and biochemistry, and electron microscopy. Bacteria surviving in SHG were characterized by: (1) capacity for rapid reactivation (growth and hydrocarbon oxidation) in fresh medium; (2) ability to synthesize surface-active compounds, which was not observed in the cultures stored without SHG); (3) elevated stress resistance (ability to grow at high Cu2+ and NaCl concentrations); (4) physiological heterogeneity of the populations, which contained the stationary hypometabolic cells, cystlike anabiotic dormant forms (DF), and ultrasmall cells; (5) occurrence of piles in many cells, which were probably used to exchange genetic material; (6) modification of the phase variants spectrum in the population growing after long-term storage in SHG; and (7) oxidation of ethanol and acetate by HOB populations stored in SHG. The combination of the physiological and cytomorphological properties of the cells surviving in SHG for long periods may indicate a new type of long-term bacterial survival, i.e., in a hypometabolic state.

show abstract