Bacterial contamination of blood components due to <i>Burkholderia cepacia</i> contamination from clorhexidine bottles

García‐Erce, José Antonio; Grasa, J.; Solano, Víctor Manuel; Gimeno, Juan José; López, A. Carrillo; Hernández, María Jesús; Marco, Maria L.; Arribas, José Luis Carles; Giralt, Manuel

doi:10.1046/j.1423-0410.2002.00195.x

Cited by 15 publications

(16 citation statements)

References 8 publications

(9 reference statements)

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“…B. cepacia is most frequently encountered in patients with cystic fibrosis, but also in other immunocompromised patients, and is usually a nosocomial infection [10]. In the latter, a common source is frequently found in the hospital, like contaminated intravenous fluid bags or even contaminated chlorhexidine bottles used for disinfection [11,12]. Nevertheless, the likelihood of clinical Burkholderia bacteraemia seemed low although our patient was immunocompromised.…”

Section: Resultsmentioning

confidence: 77%

Detection of bacterial DNA in blood samples from febrile patients: underestimated infection or emerging contamination?

Peters

Mohammadi

Vandenbroucke-Grauls

et al. 2004

FEMS Immunology &Amp; Medical Microbiology

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We applied real-time broad-range polymerase chain reaction (PCR) to detect bacteraemia in blood from febrile patients. Interpretation of amplification results in relation to clinical data and blood culture outcome was complex, although the reproducibility of the PCR results was good. Sequencing analysis of the PCR products revealed the presence of Burkholderia species DNA while no Burkholderia species grew in culture. The source of this contamination was shown to be the commercial DNA isolation kit used in the automated MagNA Pure Isolation Robot. A high degree of suspicion is required when uncommon or unexpected pathogens are diagnosed by molecular methods as clinical consequences can be serious.

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Section: Resultsmentioning

confidence: 77%

Detection of bacterial DNA in blood samples from febrile patients: underestimated infection or emerging contamination?

Peters

Mohammadi

Vandenbroucke-Grauls

et al. 2004

FEMS Immunology &Amp; Medical Microbiology

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“…where blood components were contaminated due to Burkholderia cepacie growing in chlorhexidine bottles. [4]…”

Section: Discussionmentioning

confidence: 99%

Snake in the grass: A case report of transfusion reactions due to contaminated donor arm disinfectant

Sonker

Chaudhary

2017

Asian J Transfus Sci

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Bacterial contamination of blood components remains an on-going challenge. In the majority of cases, organisms contaminating the blood components are a part of normal skin flora. Here, we report a case of bacterial contamination of blood units through contaminated donor arm disinfectant. There was a series of reactions due to random donor platelet (RDP) transfusion. The patients had features of septic transfusion reactions. On root cause analysis, spirit swabs used for disinfection of donors’ arm were identified as the culprit and presence of Clostridium difficile was established. All the blood components prepared on the dates of implicated RDP units were removed from the stock and we replaced the existing 70% alcohol disinfectant with chlorhexidine-alcohol-based antiseptic rub. Further, no such transfusion reactions were reported. Implementation of good donor arm disinfection technique in addition to the use of blood bags with diversion pouch is proposed to be best preventive strategy for resource-poor settings.

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“…Detailed investigations into various hospital outbreaks, particularly among immunocompromised or cystic fibrosis patients, have usually been associated with a contaminated liquid reservoir or moist environmental surface (Weems, 1993; Reboli et al ., 1996). Even disinfectants, such as benzalkonium chloride, povidone‐iodine or chlorhexidine, have been described as being contaminated with B. cepacia (Panlilio et al ., 1992; Oie & Kamiya, 1996; Garcia‐Erce et al ., 2002).…”

mentioning

confidence: 99%

“…However, if blood products are contaminated, leucocyte depletion is likely to increase the risk of bacterial outgrowth, and detection of gram‐negative bacteria during quality controls is an alarming finding. Garcia‐Erce et al . (2002) report the case of a transfusion‐associated septic reaction because of B. cepacia originating from a chlorhexidine‐solution used for donor arm cleansing.…”

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confidence: 99%

Pseudocontamination of blood components with Burkholderia cepacia during quality controls

Ebner¹,

Meyer²,

Schulz-Huotari

et al. 2005

Transfusion Medicine

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We report on a pseudooutbreak of Burkholderia cepacia because of the use of a contaminated disinfectant during quality controls in a university blood bank. No septic reactions associated with transfusions had been reported in patients over the last 6 months. Analysis of the individual quality control procedures showed that a disinfectant based on a quaternary ammonium compound (QAC) had been used in order to disinfect the rubber stopper of the blood culture bottle. B. cepacia was found in a sample taken from this disinfectant, which was prepared with concentrate and tap water according to the manufacturer's instructions. The four isolates (one in disinfectant and three in blood components) were found to be identical in their biochemical reactions and resistance patterns. QAC-based disinfectants are not efficacious against a part of the spectrum of gram-negatives and are therefore inadequate. After introduction of an alcohol-based preparation, no more cases of B. cepacia contamination have been identified.

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Bacterial contamination of blood components due to Burkholderia cepacia contamination from clorhexidine bottles

Cited by 15 publications

References 8 publications

Detection of bacterial DNA in blood samples from febrile patients: underestimated infection or emerging contamination?

Detection of bacterial DNA in blood samples from febrile patients: underestimated infection or emerging contamination?

Snake in the grass: A case report of transfusion reactions due to contaminated donor arm disinfectant

Pseudocontamination of blood components with Burkholderia cepacia during quality controls

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