Bacterial conversion of glycerol to beta-hydroxypropionaldehyde

Vancauwenberge, J. E.; Slininger, Patricia J.; Bothast, Rodney J.

doi:10.1128/aem.56.2.329-332.1990

Cited by 24 publications

(8 citation statements)

References 3 publications

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“…Further gut microbes with predicted glycerol/diol dehydrates were shown to form 3-HPA from glycerol. Klebsiella and Citrobacter species produced 3-HPA during growth in the presence of glycerol in addition to 1,3-PD ( 21 ), and the ability of R. gnavus to form propionate from 1,2-propanediol ( 22 ) implies the presence of an active GDH and the potential of this species to metabolize glycerol to 3-HPA.…”

Section: Microbial Glycerol Metabolism and The Reuterin Systemmentioning

confidence: 99%

Gut Microbial Glycerol Metabolism as an Endogenous Acrolein Source

Zhang

Sturla

Lacroix

et al. 2018

mBio

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Acrolein is a highly reactive electrophile causing toxic effects, such as DNA and protein adduction, oxidative stress, endoplasmic reticulum stress, immune dysfunction, and membrane damage. This Opinion/Hypothesis provides an overview of endogenous and exogenous acrolein sources, acrolein’s mode of action, and its metabolic fate. Recent reports underpin the finding that gut microbial glycerol metabolism leading to the formation of reuterin is an additional source of endogenous acrolein. Reuterin is an antimicrobial multicomponent system consisting of 3-hydroxypropionaldehyde, its dimer and hydrate, and also acrolein. The major conclusion is that gut microbes can metabolize glycerol to reuterin and that this transformation occurs in vivo. Given the known toxicity of acrolein, the observation that acrolein is formed in the gut necessitates further investigations on functional relevance for gut microbiota and the host.

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Section: Microbial Glycerol Metabolism and The Reuterin Systemmentioning

confidence: 99%

Gut Microbial Glycerol Metabolism as an Endogenous Acrolein Source

Zhang

Sturla

Lacroix

et al. 2018

mBio

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“…To decrease the inhibitory effect of accumulating 3-HPA, the use of semicarbazide to entrap 3-HPA (Vancauwenberge et al 1990) warrants further development, in particular, development of a method to recover the 3-HPA. The use of semicarbazide for entrapment would have several advantages.…”

Section: -Hpa From Renewable Resourcesmentioning

confidence: 99%

“…The 3-HPA thus generated could be easily hydrogenated using existing chemical methods, with yields of up to 99% (Brossmer and Arntz 2000;Etzkorn et al 2000). Alternatively, in the presence of semicarbazide, Klebsiella was shown to accumulate up to 46 g/l (621 mM) 3-HPA from glycerol (Vancauwenberge et al 1990). Although perhaps offering great potential for the future, so far no method has been reported for the recovery of HPA from its semicarbazide form.…”

Section: Acrylic Acidmentioning

confidence: 99%

3-Hydroxypropionaldehyde: applications and perspectives of biotechnological production

Vollenweider¹,

Lacroix²

2004

Applied Microbiology and Biotechnology

202

171

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3-hydroxypropionaldehyde (3-HPA) forms, together with HPA-hydrate and HPA-dimer, a dynamic, multi-component system (HPA system) used in food preservation, as a precursor for many modern chemicals such as acrolein, acrylic acid, and 1,3-propanediol (1,3-PDO), and for polymer production. 3-HPA can be obtained both through traditional chemistry and bacterial fermentation. To date, 3-HPA has been produced from petrochemical resources as an intermediate in 1,3-PDO production. In vivo, glycerol is converted in one enzymatic step into 3-HPA. The 3-HPA-producing Lactobacillus reuteri is used as a probiotic in the health care of humans and animals. The biotechnological production of 3-HPA from renewable resources is desirable both for use of 3-HPA in foods and for the production of bulk chemicals. The main challenge will be the efficient production and recovery of pure 3-HPA.

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“…coli [ 15 , 21 , 22 , 24 , 25 , 41 ], Lactobacillus sp. [ 42 , 43 , 44 , 45 , 46 , 47 , 48 ], and Klebsiella pneumoniae [ 49 , 50 , 51 , 52 , 53 ]. 1,3-butanediol, 3,5-dihydroxypentanal, and 3,5-dihydroxyhexanal [ 25 ]; acetate and lactate [ 15 ]; 2,3-butanediol [ 53 ] were major by-products, respectively, during 3-HP or PDO production via 3-HPA as an intermediate.…”

Section: Resultsmentioning

confidence: 99%

In Vitro One-Pot 3-Hydroxypropanal Production from Cheap C1 and C2 Compounds

Seo

Yeom

2022

IJMS

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One- or two-carbon (C1 or C2) compounds have been considered attractive substrates because they are inexpensive and abundant. Methanol and ethanol are representative C1 and C2 compounds, which can be used as bio-renewable platform feedstocks for the biotechnological production of value-added natural chemicals. Methanol-derived formaldehyde and ethanol-derived acetaldehyde can be converted to 3-hydroxypropanal (3-HPA) via aldol condensation. 3-HPA is used in food preservation and as a precursor for 3-hydroxypropionic acid and 1,3-propanediol that are starting materials for manufacturing biocompatible plastic and polytrimethylene terephthalate. In this study, 3-HPA was biosynthesized from formaldehyde and acetaldehyde using deoxyribose-5-phosphate aldolase from Thermotoga maritima (DERATma) and cloned and expressed in Escherichia coli for 3-HPA production. Under optimum conditions, DERATma produced 7 mM 3-HPA from 25 mM substrate (formaldehyde and acetaldehyde) for 60 min with 520 mg/L/h productivity. To demonstrate the one-pot 3-HPA production from methanol and ethanol, we used methanol dehydrogenase from Lysinibacillus xylanilyticus (MDHLx) and DERATma. One-pot 3-HPA production via aldol condensation of formaldehyde and acetaldehyde from methanol and ethanol, respectively, was investigated under optimized reaction conditions. This is the first report on 3-HPA production from inexpensive alcohol substrates (methanol and ethanol) by cascade reaction using DERATma and MDHLx.

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Bacterial conversion of glycerol to beta-hydroxypropionaldehyde

Cited by 24 publications

References 3 publications

Gut Microbial Glycerol Metabolism as an Endogenous Acrolein Source

Gut Microbial Glycerol Metabolism as an Endogenous Acrolein Source

3-Hydroxypropionaldehyde: applications and perspectives of biotechnological production

In Vitro One-Pot 3-Hydroxypropanal Production from Cheap C1 and C2 Compounds

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