Bacterial decontamination of liquids with pulsed electric fields

Schoenbach, K.H.; Joshi, R. P.; Stark, R.H.; Dobbs, Fred C.; Beebe, Stephen J.

doi:10.1109/94.879359

Cited by 193 publications

(112 citation statements)

References 47 publications

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“…Optimal biodegradation activity requires that the direct current does not have any negative effects on the physiology of the degrading bacteria. 'Electro-bioremediation' uses relatively weak electric fields (0.2-2 V cm 21 ) (Schwartz et al, 1997;Wick et al, 2007); a higher-voltage electrical field has been reported to have a detrimental impact, leading to cell death (Aronsson et al, 2005;Schoenbach et al, 2000). The present research focuses on the effect of low voltage on a pure culture of P. putida F1 grown in a bio electrochemical cell (BEC), using toluene as the sole carbon and energy source.…”

Section: Introductionmentioning

confidence: 99%

Effect of external voltage on Pseudomonas putida F1 in a bio electrochemical cell using toluene as sole carbon and energy source

et al. 2012

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A bio electrochemical cell (BEC) was constructed as a typical two-chamber microbial fuel cell (MFC), except that it was operated under external voltage instead of constant resistance as in an MFC. The anode chamber contained a pure culture of Pseudomonas putida F1 grown in a minimal medium containing toluene as the sole carbon and energy source. Operating the BEC under external voltages of 75, 125, 175, 250 and 500 mV (versus an Ag/AgCl reference electrode) led to increased bacterial cell growth to an OD 600 of 0.62-0.75, while the control BEC, which was not connected to external voltage, reached an OD 600 of only 0.3. Examination of the current generated under external voltages of 75, 125, 175, 250 and 500 mV showed that the maximal currents were 11, 23, 28, 54 and 94 mA m -2 , respectively. Cyclic voltammetry experiments demonstrated an anodic peak at 270 mV, which may imply oxidation of a vital molecule. The average residual toluene concentration after 147 h in the BEC operated under external voltage was 22 %, whereas in the control BEC it was 81 %. Proteome analysis of bacterial cells grown in the BEC (125 mV) revealed two groups of proteins, which are ascribed to charge transfer in the bacterial cells and from the cell to the electrode. In conclusion, operating the BEC at 75-500 mV enabled growth of a pure culture of P. putida F1 and toluene degradation even in an oxygenlimited environment.

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Section: Introductionmentioning

confidence: 99%

Effect of external voltage on Pseudomonas putida F1 in a bio electrochemical cell using toluene as sole carbon and energy source

et al. 2012

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“…The mechanism behind this electropermeabilization is electroporation, or pore formation in the cell membrane, and usually occurs for PEFs with electric fields on the order of a few kV/cm and pulse durations on the order of 0.1-10 ms. With the appropriate combination of these parameters, electroporation is irreversible and the membrane breaks down, which is desirable for applications ranging from bacterial decontamination to food processing [2]. Many applications require temporarily opening the cell membrane to normally impermeant molecules, such as electrochemotherapy and gene therapy [3].…”

Section: Introductionmentioning

confidence: 99%

Ultrashort electric pulse induced changes in cellular dielectric properties

Garner

Chen

et al. 2007

Biochemical and Biophysical Research Communications

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The interaction of nanosecond duration pulsed electric fields (nsPEFs) with biological cells, and the models describing this behavior, depend critically on the electrical properties of the cells being pulsed. Here, we used time domain dielectric spectroscopy to measure the dielectric properties of Jurkat cells, a malignant human T-cell line, before and after exposure to five 10 ns, 150 kV/cm electrical pulses. The cytoplasm and nucleoplasm conductivities decreased dramatically following pulsing, corresponding to previously observed rises in cell suspension conductivity. This suggests that electropermeabilization occurred, resulting in ion transport from the cell's interior to the exterior. A delayed decrease in cell membrane conductivity after the nsPEFs possibly suggests long-term ion channel damage or use dependence due to repeated membrane charging and discharging. This data could be used in models describing the phenomena at work. Ó 2007 Elsevier Inc. All rights reserved.Keywords: Dielectric spectroscopy; Nanosecond pulsed electric fields; Electroporation; Permeabilization; Plasma membrane; Time domain; Intracellular manipulation; Ultrashort pulsesThe permeability of the cell membrane to external molecules can be dramatically increased by applying pulsed electric fields (PEFs) above a threshold voltage [1]. The mechanism behind this electropermeabilization is electroporation, or pore formation in the cell membrane, and usually occurs for PEFs with electric fields on the order of a few kV/cm and pulse durations on the order of 0.1-10 ms. With the appropriate combination of these parameters, electroporation is irreversible and the membrane breaks down, which is desirable for applications ranging from bacterial decontamination to food processing [2]. Many applications require temporarily opening the cell membrane to normally impermeant molecules, such as electrochemotherapy and gene therapy [3]. Applying pulses with higher field strength (50-300 kV/cm) and shorter duration (10-300 ns) do not fully charge the cell membrane and instead interact primarily with the membranes of intracellular organelles [4]. These nanosecond duration PEFs (nsPEFs) cause intracellular calcium release [5,6] and apoptosis-induction in cell suspensions and in vivo tumors [5,7,8]. To better understand the mechanisms involved, attempts to develop mathematical models are underway 0006-291X/$ -see front matter Ó

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“…The first results for PEF research applied to food processing appeared in the late 1960s [1] and more recently use of the technique for liquid sterilization has widely investigated.. Noteworthy results have been reported by Old Dominion University, Washington State University and Ohio State University in the USA, Kumamoto University in Japan, the Technical University, Berlin in Germany, Technical University of Delft in the Netherlands and the Efremov Institute in Russia. In the UK important results were obtained by pulsed power groups at Strathclyde University and more recently at Loughborough University, when results providing a first step towards clarifying the mechanism for the lethality of ns-duration electric field pulses on prokariotes were reported [2].…”

Section: Introductionmentioning

confidence: 76%