Bacterial Degradation of Chlorpyrifos in Pure Cultures and in Soil

Mallick, K.; Kollah, Bharati; Banerji, Asoke; Shakil, Najam A.; Sethunathan, N.

doi:10.1007/s001289900840

Cited by 124 publications

(80 citation statements)

References 11 publications

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“…Earlier attempts to degrade chlorpyrifos by soil bacterial isolates have not been successful before 2000 (Racke et al 1994;Mallick et al 1999). In the present study, biodegradation of chlorpyrifos by three soil bacterial isolates was performed in comparison with bacterial cell growth in the MSM.…”

Section: Biodegradation Of Chlorpyrifos By Three Bacterial Strainsmentioning

confidence: 95%

Determination of selected pesticides in water samples adjacent to agricultural fields and removal of organophosphorus insecticide chlorpyrifos using soil bacterial isolates

et al. 2014

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The use of pesticide for crops leads to serious environmental pollution, therefore, it is essential to monitor and develop approaches to remove pesticide from contaminated environment. In this study, water samples were collected to monitor pesticide residues, and degradation of chlorpyrifos was also performed using soil bacteria. Identification of pesticide residues and determination of their levels were performed by high-performance liquid chromatography with photodiode array detector. Among 12 samples, 10 samples were found contaminated with pesticides. Chlorpyrifos was detected in four tested samples and concentrations ranged from 3.27 to 9.31 lg/l whereas fenitrothion ranging from (Below Detection Limit, \0.1 lg/ l) to 33.41 lg/l in the tested samples. Parathion was found in two tested samples at the concentration of 0.73 and 6.23 lg/l. None of the tested samples was found contaminated with Methoxychlor, DDT and Ethion. Three soil bacterial isolates, Pseudomonas peli BG1, Burkholderia caryophylli BG4 and Brevundimonas diminuta PD6 degraded chlorpyrifos completely in 8, 10 and 10 days, respectively, when 20 mg/l chlorpyrifos was supplied as sole source of carbon. Whereas, BG1, BG4 and PD6 took 14, 16 and 16 days, respectively, for complete removal of 50 mg/l chlorpyrifos. Chlorpyrifos degradation rates were found maximum by all three isolates at 2nd day of incubation for both tested concentrations. The results of the present study suggest the need for regular monitoring of pesticide residues in water, to protect the aquatic environment. Chlorpyrifos degrading bacterial isolates can be used to clean up environmental samples contaminated with the organophosphate pesticides.

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Section: Biodegradation Of Chlorpyrifos By Three Bacterial Strainsmentioning

confidence: 95%

Determination of selected pesticides in water samples adjacent to agricultural fields and removal of organophosphorus insecticide chlorpyrifos using soil bacterial isolates

et al. 2014

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“…Although chlorpyrifos has been widely used for agricultural and household pest control since 1965, it has been tricky isolating a degrading strain for this organophosphate. Several attempts to isolate a chlorpyrifos-degrading microbial system by repeated treatments or enrichment of soils and other media with chlorpyrifos have not been successful (Mallick et al 1999, Racke, et al 1990.The resistance of chlorpyrifos towards enhanced degradation in soil has been ascribed as a reasons for this failure. Co-metabolic degradation of chlorpyrifos by Flavobacterium sp.…”

Section: Introductionmentioning

confidence: 99%

“…Co-metabolic degradation of chlorpyrifos by Flavobacterium sp. and by a clone of Escherichia coli (with an opd gene)has been reported in liquid media (Mallick et al 1999, Richnis et al 1997, Wang et al 2002. However these microbes did not utilize chlorpyrifos as a source of energy.…”

Section: Introductionmentioning

confidence: 99%

“…However these microbes did not utilize chlorpyrifos as a source of energy. Mallick et al (Mallick et al 1999) reported degradation of chlorpyrifos by Arthrobacter species in mineral salt medium. However, the prevalence of degrading strains in soil and the pathway of degradation of chlorpyrifos by this bacterium in liquid culture are unknown.…”

Section: Introductionmentioning

confidence: 99%

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Elimination of Inhibitory Effects of Chlorpyrifos and Quinolphos on Radish and Green Gram Seed Germination by Bioremediation of Contaminated Soil: A Comparative Study

Srinivas¹,

Deviprasad²,

Manonmani³

2016

Int.J.Curr.Microbiol.App.Sci

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“…Previous reports revealed that the microbial degradation process detoxifies the pesticide contaminations and it can be effectively used to overcome the pollution problems (Bhadhade et al, 2002). Soil bacteria with the ability to degrade several pesticides have been isolated from soil showing enhanced biodegradation, which includes a metamitron degrading bacterium, Rhodococcus sp (Parekh et al, 1994); chlorpyrifos-degrading Flavobacterium sp (Mallick et al, 1999); atrazine degrading bacterium Pseudomonas sp (Ralebits et al, 2002) and an idopropane degrading bacterium Arthrobacter sp (Mercadier et al, 2002). 2,4-D is one of the most commonly used herbicides in agriculture and gardening and it exhibits serious ecological effects.…”

Section: Introductionmentioning

confidence: 99%

Identification and Growth Characterization of a Novel 2,4-D (Dichlorophenoxyacetic Acid) Degrading Bacterium Maribacter Sp Amsu Isolated From Aquaculture Effluent

Sankaralingam¹

2013

Appl Ecol Env Res

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Sankaralingam et al.: Identification and growth characterization of a novel 2,4-D (dichlorophenoxyacetic acid)However the bacterial growth was sustained and a significant death was observed with culture media containing 800mg/100ml 2,4-D and above. At regular time intervals the optical density was measured at 600nm. Growth of the isolate was determined by viable cell enumeration immediately after inoculation. The growth of Maribacter sp in medium containing 50mg/100ml to 200mg/100ml 2,4-D was not varied much during 24 to 48h, and the total viable count of 121 and 144 × 10 -4 CFU.ml -1 were observed, respectively. Media optimization results indicated that lactose (carbon) and ammonium chloride (nitrogen) added medium at pH 7 and 35 °C found to be suitable for the better growth of Maribacter sp. But lead added medium inhibited the growth of Maribacter sp. Degradation of 2,4D in culture supernatant was determined by using HPLC and the result obtained was discussed.

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Bacterial Degradation of Chlorpyrifos in Pure Cultures and in Soil

Cited by 124 publications

References 11 publications

Determination of selected pesticides in water samples adjacent to agricultural fields and removal of organophosphorus insecticide chlorpyrifos using soil bacterial isolates

Determination of selected pesticides in water samples adjacent to agricultural fields and removal of organophosphorus insecticide chlorpyrifos using soil bacterial isolates

Elimination of Inhibitory Effects of Chlorpyrifos and Quinolphos on Radish and Green Gram Seed Germination by Bioremediation of Contaminated Soil: A Comparative Study

Identification and Growth Characterization of a Novel 2,4-D (Dichlorophenoxyacetic Acid) Degrading Bacterium Maribacter Sp Amsu Isolated From Aquaculture Effluent

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