Bacterial endotoxin lipopolysaccharide induces up-regulation of glyceraldehyde-3-phosphate dehydrogenase in rat liver and lungs

Shao, Ningsheng; Ma, Xiaochang; Ling, Baodong; Wei, Yushu; Ding, Qinxue; Yang, Guang; Liu, Nongle; Wang, Huixin; Chen, Ke-ji

doi:10.1016/j.lfs.2006.06.018

Cited by 9 publications

(10 citation statements)

References 24 publications

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“…Similar findings were reported with hepatocytes exposed to LPS in vitro in the same study [23]. A microarray study using liver tissue from rats challenged with LPS demonstrated induction of the SOD2 gene 24 hours post LPS challenge however, no assessment was made at earlier time points [9]. The dose-dependent decrease in SOD gene expression that was observed in the present study 4–5 hours post LPS challenge, combined with these previously reported studies, suggest that SOD gene expression varies temporally in the liver following LPS challenge.…”

Section: Resultssupporting

confidence: 76%

“…Linear orthogonal polynomial contrasts (LOPCS) across dose were significant for CD14 ( p = 0.06), NRAMP1 ( p = 0.05), SOD ( p= 0.07) , IGFBP3 ( p = 0.03), and the GAPDH housekeeping gene ( p = 0.05), indicating that the expression of these genes was reduced across LPS doses. GAPDH has also recently been reported to not be a reliable hepatic housekeeping gene for rats challenged with LPS [9]. LOPCS across doses was also significant for MCP-1 ( p= 0.02), indicating that the expression of this gene was increased across LPS doses.…”

Section: Resultsmentioning

confidence: 97%

“…LPS ligation to CD14-TLR4 complex subsequently activates numerous cell types to secrete pro-inflammatory cytokines including IL-6. Recent studies performed with bovine MAC-T cells [15], and rat lung [16] and liver tissues [9] have shown that CD14 expression levels were largely unaffected by LPS. An earlier rodent study however, reported up-regulation of CD14 3-hours, but not 6-hours post-challenge with 4 μg/kg of LPS administered i.v [6].…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have shown that LPS induces MCP-1 gene expression in various tissues both in vivo and in vitro [25-27]. Two different LPS studies performed in vivo using the rat [9] and canine models [8] made no mention of hepatic MCP-1 induction using microarray analysis, although another chemokine, MIP-1 , was reportedly induced 4 hours post LPS challenge in the canine model [8]. A recent study however, reported hepatic MCP-1 protein expression in mice challenged with LPS [28].…”

Section: Resultsmentioning

confidence: 99%

“…A number of studies have previously used microarrays to study hepatic gene expression in rats, mice and dogs challenged with LPS; homologous arrays were used in these studies [6-9]. To date however, only two ruminant microarray studies have been performed with bovine cells stimulated with LPS, and these studies were performed in vitro [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of ovine hepatic gene expression profiles in response to Escherichia coli lipopolysaccharide using a bovine cDNA microarray

et al. 2006

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BackgroundDuring systemic gram-negative bacterial infections, lipopolysaccharide (LPS) ligation to the hepatic Toll-like receptor-4 complex induces the production of hepatic acute phase proteins that are involved in the host response to infection and limit the associated inflammatory process. Identifying the genes that regulate this hepatic response to LPS in ruminants may provide insight into the pathogenesis of bacterial diseases and eventually facilitate breeding of more disease resistant animals. The objective of this research was to profile the expression of ovine hepatic genes in response to Escherichia coli LPS challenge (0, 200, 400 ng/kg) using a bovine cDNA microarray and quantitative real-time PCR (qRT-PCR).ResultsTwelve yearling ewes were challenged iv with E. coli LPS (0, 200, 400 ng/kg) and liver biopsies were collected 4–5 hours post-challenge to assess hepatic gene expression profiles by bovine cDNA microarray and qRT-PCR analyses. The expression of CD14, C3, IL12R, NRAMP1, SOD and IGFBP3 genes was down regulated, whereas the expression of ACTHR, IFNαR, CD1, MCP-1 and GH was increased during LPS challenge. With the exception of C3, qRT-PCR analysis of 7 of these genes confirmed the microarray results and demonstrated that GAPDH is not a suitable housekeeping gene in LPS challenged sheep.ConclusionWe have identified several potentially important genes by bovine cDNA microarray and qRT-PCR analyses that are differentially expressed during the ovine hepatic response to systemic LPS challenge. Their potential role in regulating the inflammatory response to LPS warrants further investigation.

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Section: Resultssupporting

confidence: 76%

Section: Resultsmentioning

confidence: 97%