Bacterial fibronectin-binding proteins and endothelial cell surface fibronectin mediate adherence of Staphylococcus aureus to resting human endothelial cells

Peacock, Sharon J.; Foster, Timothy J.; Cameron, Brian; Berendt, Anthony R.

doi:10.1099/00221287-145-12-3477

Cited by 207 publications

(186 citation statements)

References 54 publications

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“…ECM binding, including Fn binding, is regularly elicited by pathogens to adhere to host cells (19,48,65). Moreover, Fn can also serve as a mediator to induce endocytosis and initiate the entry of bacteria when it binds to bacterial Fn-binding proteins (65,66).…”

Section: Discussionmentioning

confidence: 99%

Manganese Binds to Clostridium difficile Fbp68 and Is Essential for Fibronectin Binding

Lin¹,

Kuo²,

Koleci³

et al. 2011

Journal of Biological Chemistry

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Clostridium difficile is an etiological agent of pseudomembranous colitis and antibiotic-associated diarrhea. Adhesion is the crucial first step in bacterial infection. Thus, in addition to toxins, the importance of colonization factors in C. difficileassociated disease is recognized. In this study, we identified Clostridium difficile is a Gram-positive, spore-forming anaerobic bacterium that infects people and multiple animal species. Colonization of the gastrointestinal tract may be asymptomatic or cause a variety of disorders, including mild diarrhea, pseudomembranous colitis, and antibiotic-associated diarrhea (1). Recent outbreaks in North America and Europe document the seriousness of C. difficile infection (2). C. difficile toxins, including toxin A, toxin B, and a recently identified toxin, binary ADP-ribosyltransferase toxin C. difficile transferase, are thought to be the primary virulence factors that mediate C. difficile-associated disease (3). Because C. difficile colonizes gastrointestinal tissues and enterocyte-like Caco-2 cells (4, 5), colonization factors are also recognized as important virulence factors of C. difficile. A variety of colonization factors has been identified, including the following: capsule (6); proteolytic enzymes (7, 8); S-layer proteins P36 and P47 (9); adhesins such as SLPA, CCAP6, Fbp68, and 12-kDa protein (4, 9 -14); flagellins such as FliC and FliD (15, 16); and GroEL chaperones (17,18).Adhesion is the first step in bacterial infection, and several adhesins known as microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) 2 contribute to this step (19). MSCRAMMs located on bacterial surfaces mediate adhesion by binding to various extracellular matrices including fibronectin (Fn), laminin, collagen, elastin, and proteoglycan on host surfaces (19). Loss of some of these genes may attenuate virulence, indicating that MSCRAMMs are pivotal mediators of bacterial disease (20). Although a number of MSCRAMMs have been investigated in other bacterial pathogens, only a few clostridial adhesins, such as Fbp68, have been characterized, and the colonization mechanisms of C. difficile are still poorly understood (11).Manganese-binding proteins are important players in bacterial physiology by participating in cation homeostasis (21), carbon metabolism to promote nutrient acquisition (22), signal transduction (23), resistance to oxidative stress (24), and nutrient-deprived stress (25). In addition, the interaction of some bacterial adhesins and ECM components can be modulated by metal ions such as calcium (26,27). To date, the ability of manganese to mediate bacterial adhesion has not been reported.Previously, Fbp68 on the surface of C. difficile was shown to serve as an adhesin by binding to Fn, fibrinogen, and vitronectin (11). Interestingly, antibody to Fbp68 can be detected in sera from patients with C. difficile-associated disease, indicating that Fbp68 can induce a host immune response during C. difficile infection (28). Structural analysis of Fbp68 indicates t...

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Section: Discussionmentioning

confidence: 99%

Manganese Binds to Clostridium difficile Fbp68 and Is Essential for Fibronectin Binding

Lin¹,

Kuo²,

Koleci³

et al. 2011

Journal of Biological Chemistry

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“…Fn also serves as a mediator to induce endocytosis and initiate the entry of bacteria upon binding to bacterial surface proteins (51,52). We tested whether reducing the expression of Fn on Caco-2 cells would impair the ability of Ag85 or Ag85-expressing MAP K-10 to bind to the Caco-2 cells.…”

Section: Volume 287 • Number 3 • January 13 2012mentioning

confidence: 99%

Novel Mycobacteria Antigen 85 Complex Binding Motif on Fibronectin

Kuo

Bell

Hsieh

et al. 2012

Journal of Biological Chemistry

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“…The ability to bind to host extracellular matrix and plasma components promotes adhesion to host tissues, evasion of host defence mechanisms and invasion of epithelial and endothelial cells (Foster & Höök, 1998;Nilsson et al, 1998;Peacock et al, 1999). More recently, surface proteins have also been shown to play roles in biofilm formation (Cucarella et al, 2001) and binding of host transferrin (Taylor & Heinrichs, 2002).…”

Section: Introductionmentioning

confidence: 99%

Characterization of novel LPXTG-containing proteins of Staphylococcus aureus identified from genome sequences

et al. 2003

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Bacterial fibronectin-binding proteins and endothelial cell surface fibronectin mediate adherence of Staphylococcus aureus to resting human endothelial cells

Abstract: Adhesion of Staphylococcus aureus to human endothelial cells is implicated in

Cited by 207 publications

References 54 publications

Manganese Binds to Clostridium difficile Fbp68 and Is Essential for Fibronectin Binding

Manganese Binds to Clostridium difficile Fbp68 and Is Essential for Fibronectin Binding

Novel Mycobacteria Antigen 85 Complex Binding Motif on Fibronectin

Characterization of novel LPXTG-containing proteins of Staphylococcus aureus identified from genome sequences

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