Bacterial halo blight of coffee crop: aggressiveness and genetic diversity of strains

The genetic variability and aggressiveness of Brazilian Erwinia psidii isolates from Eucalyptus spp. was studied and compared with reference isolates from guava (Psidium guajava). Repetitive element sequence (rep)‐based PCR markers of 101 isolates from Eucalyptus spp. and five from guava showed that the populations of E. psidii displayed a relatively low genetic variability. No correlation of genetic clustering based on rep‐PCR analysis with geographic origin or host of origin was observed, indicating that genome rearrangements associated with adaptation to a particular host were not detected by these molecular markers. A higher genotypic richness was detected in the Mato Grosso do Sul population, probably reflecting a pathogen dissemination associated with the recent expansion in eucalypt plantations. Wilcoxon and ANOVA tests of disease severity data indicated differences in aggressiveness among isolates and an isolate × clone interaction. The area under the disease progress curve (AUDPC) and disease severity for some isolates were significantly different between two susceptible clones tested. Notably, isolate LPF681 from guava was not able to cause disease on a susceptible Eucalyptus urophylla clone, suggesting that some co‐evolution between pathogen and host has taken place. The variability in aggressiveness and virulence among isolates of E. psidii observed in this study will be important for the establishment of appropriate screening approaches to select for disease resistance.

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“…For instance, Maciel et al . () found variation in aggressiveness among Pseudomonas syringae pv. garcae isolates obtained from Coffea arabica .…”

Section: Discussionmentioning

confidence: 99%

“…Variation in aggressiveness among isolates has already been reported for other bacterial phytopathogens. For instance, Maciel et al (2018) found variation in aggressiveness among Pseudomonas syringae pv. garcae isolates obtained from Coffea arabica.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and aggressiveness of Erwinia psidii on Eucalyptus spp. in Brazil

et al. 2018

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“…Mwangi et al (2018) reported variability in the 16S rDNA sequence and found that the most aggressive strains clustered together in the phylogenetic tree, but no correlation with geographic origin was observed. In contrast, Maciel et al (2018) when using rep-PCR to evaluate the genetic diversity of Brazilian and Kenyan strains, found clustering according to geographic origin, although no clear correlation with aggressiveness was detected. A major caveat of those studies is that a small number of strains was evaluated and it is difficult to draw clear conclusions.…”

Section: Taxonomy Host Range and Genetic Diversitymentioning

confidence: 74%

“…Aggressiveness variation among Psgc strains is supported by studies conducted in Kenya and Brazil (Ithiru et al ., ; Rodrigues et al ., ; Maciel et al ., ; Mwangi et al ., ). Interestingly, Kairu () found that cultivar SL 28 was resistant to Brazilian strains but susceptible to Kenyan strains, suggesting a possible virulence variability among bacterial populations.…”

Section: Bacterial Halo Blightmentioning

confidence: 97%

“…In contrast, Maciel et al . () when using rep‐PCR to evaluate the genetic diversity of Brazilian and Kenyan strains, found clustering according to geographic origin, although no clear correlation with aggressiveness was detected. A major caveat of those studies is that a small number of strains was evaluated and it is difficult to draw clear conclusions.…”

Section: Bacterial Halo Blightmentioning

confidence: 97%

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Coffee bacterial diseases: a plethora of scientific opportunities

Badel

Zambolim

2019

Plant Pathology

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Coffee is a very important crop for several tropical countries across different continents. The diseases bacterial halo blight (BHB), bacterial leaf spot (BLS), bacterial leaf blight (BLB) and coffee leaf scorch (CLS), caused by the bacterial pathogens Pseudomonas syringae pv. garcae (Psgc), P. syringae pv. tabaci (Psta), Pseudomonas cichorii (Pch) and Xylella fastidiosa subsp. pauca (Xfp), respectively, cause significant reductions in coffee production, although other minor bacterial diseases have also been reported in some countries. Little research progress has been made on aspects that are relevant for control and management of these diseases. In all cases, there is an urgent need to develop rapid and more reliable methods for early detection of the pathogens in order to minimize their negative impact on coffee production. Because of the high rate of intra‐ and intersubspecific recombination occurring in X. fastidiosa, a permanent revision of the detection methods is necessary. Greater efforts should be made to understand the genetic and virulence diversity of Psgc, Psta and Pch populations. Early studies reported the identification of potential sources of resistance against Psgc and Psta, but, to date, no resistance gene has been isolated. Little effort has been made to understand the biology and molecular mechanisms underlying the interaction between Coffea spp. and these pathogenic bacteria. This review discusses the recent progress on the molecular mechanisms used by these bacteria to cause diseases on other plant species, in order to provide a guideline for the establishment of future research programmes.

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Current Challenges and Genomic Advances Toward the Development of Coffee Genotypes Resistant to Biotic Stress

Ariyoshi

Oliveira

Shigueoka³

et al. 2022

Genomic Designing for Biotic Stress Resistant Technical Crops

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Bacterial halo blight of coffee crop: aggressiveness and genetic diversity of strains

Cited by 11 publications

References 13 publications

Genetic diversity and aggressiveness of Erwinia psidii on Eucalyptus spp. in Brazil

Genetic diversity and aggressiveness of Erwinia psidii on Eucalyptus spp. in Brazil

Coffee bacterial diseases: a plethora of scientific opportunities

Current Challenges and Genomic Advances Toward the Development of Coffee Genotypes Resistant to Biotic Stress

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