Bacterial Hemoglobin gene (vgb) Transformed into Escherichia coli enhances Lead-Uptake and Minimizes it’s Adsorption

Khliefat, Khaled; Homady, Merza H.

doi:10.3923/pjbs.2000.1480.1483

Cited by 4 publications

(4 citation statements)

References 12 publications

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“…As mentioned previously only three compounds (cupric chloride dihydrate, cupric nitrate 3 hydrate and cupric sulfate) with concentration (300 and 1000 ppm) were used in studding the profile of growth curves.The viable cells were precisely enable us to see the effect of copper compounds on the growth kinetics of the bacteria Pseudomonas aeruginosa. The bioaviability of heavy metals in the broth is more than in the plates (Khleifat and Homady, 2000). Generally the results here were in agreement with the results of biomass.…”

Section: Growth Curvessupporting

confidence: 91%

Effect of Copper Chemical Form on The Growth of Pseudomonas aeruginosa Isolated from Burned Patients and on Its Cu Uptake

Al-Tarawneh

Qaralleh

Al-limoun

et al. 2019

J. basic appl. res. biomed.

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The main object of this investigation was to shed light on the information on the level of Cu uptake by Pseudomonas aeruginosa that has been previously isolated from patients using Gram-negative E. aerogenes ans a Gram-positive Bacillus thuringiensis as a control for Cu uptake measurements. Cu uptake data showed that maximum Cu uptake was obtained by the Pseudomonas aeruginosa. The metal uptake was dependent on the type of biosorbent with different accumulation affinities toward the tested chemical form of the copper. Cells harvested at exponential growth phase showed slightly higher Cu uptake than at stationary phase, which reflect that the Cu uptake is metabolism dependent-process. The increasing order of affinity of the cupric chloride and cupric sulphate towards the three genera were almost constant. However, where cupric nitrate was used, the copper uptake behavior in Pseudomonas aeroginosa was not changed from those results with the other two chemical forms, whereas with E. aerogenes & B. thuringiensis, different Cu uptake behaviors were observed. In B. thuengenesis, the copper uptake rate as a function of initial Cu concentration were shown to increase with increasing the initial concentration of Cu, constantly above 320 ppm as compared with others. In Enterobacter with cupric nitrate, different bahavior was also shown in which the copper uptake decline sharply beyond the 320 ppm. These results reflect that the Cu uptake in P. aeruginosa is with different mechanisms as compared with other tested bacteria

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Section: Growth Curvessupporting

confidence: 91%

Effect of Copper Chemical Form on The Growth of Pseudomonas aeruginosa Isolated from Burned Patients and on Its Cu Uptake

Al-Tarawneh

Qaralleh

Al-limoun

et al. 2019

J. basic appl. res. biomed.

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“…13c). It was reported that optimum pH for the biodegradation of different aromatic compounds was different from one bacterium to another, for example, pH ranges between 8 and 11 for biodegradation of phenol and catechol by the bacterium Halomonas campisalis (Alva and Peyton, 2003) and for the biodegradation of phenol by Klebsiella oxytoca, the pH was 6.8 (Shawabkeh et al, 2007;(Khleifat et al, 2000;Khleifat et al, 2001;Khleifat et al, 2006;Al Qaisi et al, 2014;Aljundi et al, 2010a;Khleifat et al, 2022a;Khlaifat et al, 2019;.…”

Section: Effect Of Ph Of Culture Media On Biodegradation Of 2-cbamentioning

confidence: 99%

Biodegradation of 2- Chlorobenzoic Acid and its other substitutes

AL-Quraishi¹

2022

J. basic appl. Res. Biomed.

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Because of the widespread use of these halogenated organic compounds in agriculture and industry, considerable quantities of these compounds' byproducts are discharged into the environment. These chemicals are known as halogenated pesticides. There is one group that, based on the chemical structures of these compounds, is thought to be the most tenacious and poisonous of all the groups. This group is known as the group. A. hydrophila, which was shown to be capable of utilising chlorobenzoate chemicals as a carbon and energy source, was isolated from wastewater treatment plant effluent in Petra, Jordan. Different biodegradation rates (4-chlorobenzoic acid, 5 M/hr; 3,4-dichlorobenzoic acid, 15.5 M/hr; 2-chlorobenzoic acid, 41 M/hr; 3-chlorobenzoic acid, 65 M/hr) were used to achieve these capacities. The release of chloride, the disappearance of the substrate, and finally the development of bacterial cells on these substrates were used to monitor the ability to degrade. Analyses of the ortho or meta ring-cleavage of these aromatic compounds were conducted on A. hydrophila dioxygenases that were physiologically activated by chlorobenzoic acid compounds. The fact that only one 2-dioxygenase activity was found suggests that the ortho route is used for the cleavage. The optimal conditions for 2-CBA chemical breakdown were 3 mM substrate concentration, 25 oC, pH 7, and 200 l inoculum size. Differently from how they affected chloride and cell mass synthesis, the carbon sources had an impact on the breakdown of 2-CBA. Utilized nitrogen sources decreased 2-CBA's ability to degrade and its ability to release chlorine. However, the biodegradation between the 40 and 80 hours was slightly enhanced by the nitrogen source L-proline.

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“…After treatment with lectin at concentrations of 15.1 g/mL to 1000 g/mL, the total biomass of the biofilm reduced depending on the concentration; treatment at 1000 g/mL showed an inhibition of more than 80%. (Khleifat et al, 2000;Khleifat et al, 2001;Khleifat et al, 2006;Aljundi et al, 2010a;Khleifat et al, 2022a;Khlaifat et al, 2019;Varijakzhan et al, 2021a). It was discovered, however, that some of the marine sponge chemicals were less efficient against Gram-negative bacteria.…”

Section: Antibacterial Activity Of Various Compounds Isolated From Ma...mentioning

confidence: 99%

Marine sponge's biological activities: Their Biotechnological Uses

Al-Adaileh

Al-Madadheh

Almashaleh

2022

J. basic appl. Res. Biomed.

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The most of the time, marine sponges are an invertebrate species found in the tropics, subtropics, and temperate zones of the oceans and seas. They are acknowledged as being among the most important sources of bioactive chemicals, which are found in marine habitats and can then be recovered from those settings. These bioactive chemicals are found in marine environments and can then be extracted from those environments. It is common knowledge that the chemicals that are extracted from these sponges demonstrate a wide range of bioactivities, such as antibacterial activity, anticancer activity, and general cytotoxicity. These bioactivities can be found in the isolated chemicals. In this article, we discuss the bioactive chemicals that have been found in marine sponges and their potential applications. These compounds have the potential to serve as antibacterial, antiviral, and antimalarial agents against human infections as well as fish pathogens in the aquaculture industry. In addition, these compounds may also operate as antifungal agents. Sponge organisms found in the ocean have also been shown to have these chemical compounds. This article discusses the importance of marine sponges to the fields of chemistry, microbiology, cell biology, and molecular biology from a biotechnological standpoint. Researchers of marine natural products have discovered new potential medications as a result of their efforts to exploit the remarkable chemical variety that can be found in sponges.

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Bacterial Hemoglobin gene (vgb) Transformed into Escherichia coli enhances Lead-Uptake and Minimizes it’s Adsorption

Cited by 4 publications

References 12 publications

Effect of Copper Chemical Form on The Growth of Pseudomonas aeruginosa Isolated from Burned Patients and on Its Cu Uptake

Effect of Copper Chemical Form on The Growth of Pseudomonas aeruginosa Isolated from Burned Patients and on Its Cu Uptake

Biodegradation of 2- Chlorobenzoic Acid and its other substitutes

Marine sponge's biological activities: Their Biotechnological Uses

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