Bacterial Ice Crystal Controlling Proteins

Lorv, Janet; Rose, David R.; Glick, Bernard R.

doi:10.1155/2014/976895

Cited by 82 publications

(59 citation statements)

References 111 publications

(369 reference statements)

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“…Multiplying this fraction by the PBAP concentration measured by the WIBS gives an INP concentration of 0.5 L −1 at this temperature. As Pseudomonas syringae is amongst the most active biological INPs studied thus far (Lorv et al, 2014), this value may be considered an upper estimate for biological INPs at this temperature. Though it is possible to classify WIBS measurements into different PBAP types, even within simple metaclass discrimination (bacteria, fungal spores, and pollen) the uncertainty in the derived concentrations can be large, e.g.…”

Section: Sources Of Inpsmentioning

confidence: 99%

Aerosol measurements during COPE: composition, size, and sources of CCN and INPs at the interface between marine and terrestrial influences

et al. 2016

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Abstract. Heavy rainfall from convective clouds can lead to devastating flash flooding, and observations of aerosols and clouds are required to improve cloud parameterisations used in precipitation forecasts. We present measurements of boundary layer aerosol concentration, size, and composition from a series of research flights performed over the southwest peninsula of the UK during the COnvective Precipitation Experiment (COPE) of summer 2013. We place emphasis on periods of southwesterly winds, which locally are most conducive to convective cloud formation, when marine air from the Atlantic reached the peninsula. Accumulation-mode aerosol mass loadings were typically 2-3 µg m −3 (corrected to standard cubic metres at 1013.25 hPa and 273.15 K), the majority of which was sulfuric acid over the sea, or ammonium sulfate inland, as terrestrial ammonia sources neutralised the aerosol. The cloud condensation nuclei (CCN) concentrations in these conditions were ∼ 150-280 cm −3 at 0.1 % and 400-500 cm −3 at 0.9 % supersaturation (SST), which are in good agreement with previous Atlantic measurements, and the cloud drop concentrations at cloud base ranged from 100 to 500 cm −3 . The concentration of CCN at 0.1 % SST was well correlated with non-sea-salt sulfate, meaning marine sulfate formation was likely the main source of CCN. Marine organic aerosol (OA) had a similar mass spectrum to previous measurements of sea spray OA and was poorly correlated with CCN.In one case study that was significantly different to the rest, polluted anthropogenic emissions from the southern and central UK advected to the peninsula, with significant enhancements of OA, ammonium nitrate and sulfate, and black carbon. The CCN concentrations here were around 6 times higher than in the clean cases, and the cloud drop number concentrations were 3-4 times higher.Sources of ice-nucleating particles (INPs) were assessed by comparing different parameterisations used to predict INP concentrations, using measured aerosol concentrations as input. The parameterisations based on total aerosol produced INP concentrations that agreed within an order of magnitude with measured first ice concentrations at cloud temperatures as low as −12 • C. Composition-specific parameterisations for mineral dust, fluorescent particles, and sea spray OA were 3-4 orders of magnitude lower than the measured first ice concentrations, meaning a source of INPs was present that was not characterised by our measurements and/or one or more of the composition-specific parameterisations greatly underestimated INPs in this environment.

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Section: Sources Of Inpsmentioning

confidence: 99%

Aerosol measurements during COPE: composition, size, and sources of CCN and INPs at the interface between marine and terrestrial influences

et al. 2016

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“…7,8 Generally, ina C bacteria are Gram-negative, epiphytic and pathogenic as Pseudomonas syringae, Pseudomonas putidia, Erwinia herbicola, Erwinia ananas, Xanthomonas campestris, or Pantoea ananatis among others. [7][8][9][10][11][12][13][14][15][16][17] The well characterized plant pathogen Pseudomonas syringae represents one of the most efficient INA bacterial ice nucleus known, initiating plant damaging ice formation at temperatures of ¡2 C. 11,18 Aside from the habitat of an epiphytic plant pathogen, Pseudomonas syringae, as well as other INA bacteria were found in clouds, rain, snow and streams indicating that they are disseminated with the earth hydrological cycle. [19][20][21][22] Airborne ice-active bacteria are involved in cloud condensation -acting as cloud condensation nuclei (CCN) -and precipitation.…”

Section: Introductionmentioning

confidence: 99%

Functional display of ice nucleation protein InaZ on the surface of bacterial ghosts

Kassmannhuber

Rauscher²,

Schöner³

et al. 2017

Bioengineered

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In a concept study the ability to induce heterogeneous ice formation by Bacterial Ghosts (BGs) from Escherichia coli carrying ice nucleation protein InaZ from Pseudomonas syringae in their outer membrane was investigated by a droplet-freezing assay of ultra-pure water. As determined by the median freezing temperature and cumulative ice nucleation spectra it could be demonstrated that both the living recombinant E. coli and their corresponding BGs functionally display InaZ on their surface. Under the production conditions chosen both samples belong to type II ice-nucleation particles inducing ice formation at a temperature range of between ¡5.6 C and ¡6.7 C, respectively. One advantage for the application of such BGs over their living recombinant mother bacteria is that they are non-living native cell envelopes retaining the biophysical properties of ice nucleation and do no longer represent genetically modified organisms (GMOs).

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“…These findings may be applicable in the production of natural pesticides (Tang et al 2004. ; Lee et al 2016), frost prevention (Lorv et al 2014), food uses (James et al 2015) and artificial snow (Cid et al 2016).…”

Section: Discussionmentioning

confidence: 93%

Ice nucleation active bacteria from pistachio in Kerman Province, Iran

et al. 2018

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Frost damage is one of the major factors threatening pistachio production in southern part of Iran. In the present study, an attempt was made to screen and characterize ice nucleation bacteria associated with frost damage among some predominant epiphytic microflora. A large number of epiphytic bacteria was isolated from different regions in Kerman province. 1200 bacterial isolates were obtained from bud, twig, leaf and green fruit surfaces. Sixty-four strains exhibited ice nucleation activity. Among these, a vast microbial diversity was observed. For preliminary characterization of the isolates, certain key biochemical and pathogenicity tests were performed. The preserved cultures were often checked for ice formation and loss of ice nucleation activity was found in most cultures. Furthermore, factors affecting the increment of ice nucleation activity were studied and the isolates that recovered their ice nucleation activity were selected for sequence analyses of 16SrDNA, rpoD and recA genes, then compared with other isolates in NCBI GenBank using blasts methods. Pseudomonas fragi, P. putida, P. moraviensis, P. virdiflava and Pantoea agglomerans were the major predominant ice nucleation active bacteria established on plant surfaces. This work reports for the first time the presence of two ice nucleation active bacteria, P. fragi and P. moraviensis, on pistachio plants.

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Bacterial Ice Crystal Controlling Proteins

Cited by 82 publications

References 111 publications

Aerosol measurements during COPE: composition, size, and sources of CCN and INPs at the interface between marine and terrestrial influences

Aerosol measurements during COPE: composition, size, and sources of CCN and INPs at the interface between marine and terrestrial influences

Functional display of ice nucleation protein InaZ on the surface of bacterial ghosts

Ice nucleation active bacteria from pistachio in Kerman Province, Iran

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