Bacterial Imprinting at Pickering Emulsion Interfaces

Abstract

The tendency of bacteria to assemble at oil-water interfaces can be utilized to create microbial recognition sites on the surface of polymer beads. In this work, two different groups of bacteria were first treated with acryloyl-functionalized chitosan and then used to stabilize an oil-in-water emulsion composed of cross-linking monomers that were dispersed in aqueous buffer. Polymerization of the oil phase followed by removal of the bacterial template resulted in welldefined polymer beads bearing bacterial imprints. Chemical passivation of chitosan and cell displacement assays indicate that the bacterial recognition on the polymer beads was dependent on the nature of the pre-polymer and the target bacteria. The functional materials for microbial recognition show great potential for constructing cell-cell communication networks, biosensors, and new platforms for testing antibiotic drugs.

Label-Free Detection of Escherichia coli Based on Thermal Transport through Surface Imprinted Polymers

“…Platforms based on optical techniques such as ELISA, confocal and fluorescence microscopy have proven to be very selective and extremely sensitive. [29][30][31][32][33] However, since these techniques rely on expensive equipment, operated by experienced users in a lab environment and are difficult to miniaturize their applicability for point-of-care testing is limited. Therefore, labelfree, low-cost alternatives have been developed based on classical biosensor read-out platforms based on microgravimetrical, [34][35][36] electrochemical, [37][38][39] and optical detection principles.…”

Pickering emulsion-based molecular imprinting

“…细 菌具有和胶体颗粒一样的大小尺寸, 也有趋向于存 在油-水界面的性质 [69] . Zhu等人 [27] a) 羟甲基丙烯酰胺; b) 亚甲基双丙烯酰胺; c) 聚苯乙烯-聚甲基丙烯酸 图 4 (网络版彩色)Pickering乳液聚合合成细菌印迹示意图 [70] Figure 4 (Color online) Schematic illustration for bacterial imprinting in Pickering emulsion [70] . Figure 5 SEM images of different MIP particles.…”

Epitope imprinting of iron binding protein of Neisseria meningitidis bacteria through multiple monomers imprinting approach

“…Moreover, this device is a widely used sensing device when combined with MIP materials. In the reported studies describing epitope molecularly imprinted polymers (EMIP)‐based QCM sensors, the advantageous stability and selectivity of MIP materials and the sensitivity of the QCM sensor might combine epitope imprinting approach and surface imprinting effectively …”