Bacterial inoculum enhances keratin degradation and biofilm formation in poultry compost

Ichida, Jann M.; Krizova, Lucie; LeFevre, Colleen A; Keener, H. M.; Elwell, D. L.; Burtt, Edward H.

doi:10.1016/s0167-7012(01)00302-5

Cited by 129 publications

(91 citation statements)

References 15 publications

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“…OM was assessed by determining the loss on ignition at 430°C for 24 h [21]. The fresh samples were analysed for GI in a 1:10(w/v) water-soluble extract, E. coli were fermented by the multiple-tube fermentation technique, and mortality of ascarid egg were counted using the saline saturated solution floating concentrated technique [22,23]. TN and TC were determined by automatic microanalysis [24], then C/N ratio was calculated by them, and TP and TK were measured by inductively coupled The sample for determination of water content and pH was wet basis; the sample for determination of organic matter, total N and total P was dry basis; and values in parentheses are standard deviations of all physiochemical analyses of the raw materials, n = 3 plasma-atomic emission spectrometry (ICP-AES) with a microwave digestion method [21].…”

Section: Experimental Methodsmentioning

confidence: 99%

Comparison and Evaluation of Co-composting Corn Stalk or Rice Husk with Swine Waste in China

Gao

Zhou

Wang

et al. 2015

Waste Biomass Valor

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An experimental composting system was employed to study the effect of different proportions of corn stalk, rice husk and swine waste on composting efficiency and final compost quality. Three dry mass ratios were designed with biological agent addition as blank contrast. After 84 days of a static aerobic composting process, full-scale comparison was investigated in maturity, organic nutrient and sanitarian properties. The results showed that the treatment of corn stalk co-composting with swine waste at 1:1.5 ratio with the addition of a biological agent was the first to achieve maturity and had a higher level of organic fertiliser quality, and hygienic indicators of the compost product were satisfied within the relevant standards for harm. Meanwhile, the effect of the biological agent on acceleration of degradation was verified in corn stalk composting. This study found rice husk compost did not reach ideal high temperature. The maturity effect of the final product was relatively poor. Except the treatment of rice husk mixing swine manure at 1:2 ratio with biological agent, other treatments of rice husk had lower and lesseffective products, no positive effect was observed in the rice husk compost. At the end of this paper some suggestions were given to develop new approaches for rice husk utilisation and reduce the cost of corn stalk co-composting.

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Section: Experimental Methodsmentioning

confidence: 99%

Comparison and Evaluation of Co-composting Corn Stalk or Rice Husk with Swine Waste in China

Gao

Zhou

Wang

et al. 2015

Waste Biomass Valor

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“…The enzymes are widely used not only in chemical and medical industries, but also in animal feed industry and basic biological science (Ichida, 2001). Several studies on B. licheniformis (Ichida, 2001;Evans, 2000;Zhao et al, 1998) confirmed the high activity of kertinase produced. Therefore enzymatic hydrolysis of feather wastes or dead chickens could be a safe method of recycling these organic materials.…”

Section: Resultsmentioning

confidence: 99%

Feather wastes digestion by new isolated strains Bacillus sp. in Morocco

Zerdani¹,

Faïd²,

Malki³

2004

Afr. J. Biotechnol.

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Eight strains of Bacillus were isolated from non treated soil, characterized and used for the digestion of feather wastes in the laboratory. Non-protein nitrogen (NPN) and total protein (TP) were determined during the incubation time and the microbial counts of the different strains during feather hydrolysis were also monitored. Results of the screening test showed that the solid pieces of feather were completely digested by all the strains. The most efficient isolated strain selected was compared with Bacillus subtilis ATCC 6633. Results showed that the total protein decreased from 13.6% to 1.92 % with the isolated strain, and from 12.25 % to 2.99% with the standard strain. The NPN reached a concentration of 43.2mg/100g and 20.5 mg/100g with the isolated and standard strains, respectively.

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“…The first pretreatment step was Waste Biomass Valor (2017) 8:527-537 529 developed in order to remove tissue remnants, non-keratinous proteins and lipids from the crude pig bristle bulk. It was performed by conducting a short-term culture of B. cereus PCM 2849 in a mixture consisting of pig bristle 10 g, distilled water 250 cm 3 and bacterial inoculum in nutrient broth 50 cm 3 . Afterwards, the solids were separated, washed with tap water and dried.…”

Section: Pretreatment Of Pig Bristlesmentioning

confidence: 99%

“…Composting of keratinous waste appears as a cost-effective and ecologically safe method, especially when closed bioreactors for dynamic composting are used. Nevertheless, to achieve beneficial efficiency of keratin biodegradation it is essential to incorporate keratin-degrading bacteria, fungi or actinomycetes [1][2][3]. Biomethanation, anaerobic conversion of organic waste by methanogenic bacteria, is also applicable for feather waste processing.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Degradation of Pretreated Pig Bristles with Crude Keratinase of Bacillus cereus PCM 2849

Łaba

Chorążyk

Pudło

et al. 2016

Waste Biomass Valor

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Purpose The aim of the study was to apply and optimize the process of bioconversion of pig bristle waste using keratinolytic enzymes of Bacillus cereus PCM 2849, and to evaluate the amino acid composition of the resultant hydrolysate. Methods Hydrolysis with concentrated culture fluid of B. cereus was applied for bioconversion of pig bristles, after thermo-chemical pretreatment with sulfite. The effect of substrate concentration, sulfite concentration during pretreatment and reaction temperature on the release of amino acids was determined using Box-Behnken design. Amino acid composition of the obtained hydrolysate was determined by HPLC. Structural condition and substructural changes of the residual substrate were evaluated with SEM microscopy and FTIR spectroscopy. Results The applied enzymatic preparation for bristle biodegradation was verified to contain multiple proteases of a wide molecular weight range. A regression model was developed, in which influential parameters were: linear effect of substrate concentration, followed by quadratic effects of reaction temperature, substrate concentration and pretreatment. Optimum reaction conditions were also determined. The resultant hydrolysate was rich in branched-chain amino acids. Residual substrate was detriorated and sulfitolytic cleavage of disulfides and alteration of protein secondary structures was confirmed. Conclusions Application of B. cereus crude keratinase allowed for partial hydrolysis of pig bristles, preceded by sulfitolytic pretreatment. A regression model was built to describe the process of hydrolysis to release free amino acids, at constant enzyme load. Hydrolysis in given conditions allowed to obtain hydrolysate rich in branched chain amino acids. The presented process poses an alternate way of management over pig bristles, a hard-to-degrade keratinous waste.

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Bacterial inoculum enhances keratin degradation and biofilm formation in poultry compost

Cited by 129 publications

References 15 publications

Comparison and Evaluation of Co-composting Corn Stalk or Rice Husk with Swine Waste in China

Comparison and Evaluation of Co-composting Corn Stalk or Rice Husk with Swine Waste in China

Feather wastes digestion by new isolated strains Bacillus sp. in Morocco

Enzymatic Degradation of Pretreated Pig Bristles with Crude Keratinase of Bacillus cereus PCM 2849

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