Bacterial Interactions with Aspergillus fumigatus in the Immunocompromised Lung

Margalit, Anatte; Carolan, James C.; Kavanagh, Kevin

doi:10.3390/microorganisms9020435

Cited by 14 publications

(14 citation statements)

References 139 publications

(119 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Apart from P. aeruginosa, interactions between A. fumigatus and other bacterial infections, such as Staphylococcus aureus, Streptococcus pneumoniae, Klebsiella pneumoniae and Stenotrophomonas maltophilia, do not only lead to superinfection but also increase the chance of A. fumigatus infection evolving into pulmonary disease in PwCF [60]. In addition, in PwCF, a positive fungal culture is often associated with a positive culture for nontuberculous mycobacteria [61,62], a combination that seems to predispose to the development of chronic pulmonary aspergillosis [63,64].…”

Section: Interactionsmentioning

confidence: 99%

Aspergillus-related lung disease in people with cystic fibrosis: can imaging help us to diagnose disease?

Elders

Warris

et al. 2021

Eur Respir Rev

View full text Add to dashboard Cite

In people with cystic fibrosis (PwCF), viscous sputum and dysfunction of the mucociliary escalator leads to early and chronic infections. The prevalence of Aspergillus fumigatus in sputum is high in PwCF and the contribution of A. fumigatus to the progression of structural lung disease has been reported. However, overall, relatively little is known about the contribution of A. fumigatus to CF lung disease. More knowledge is needed to aid clinical decisions on whether to start antifungal treatment. In this review, we give an overview of A. fumigatus colonisation and infection in PwCF and the different types of pulmonary disease caused by it. Furthermore, we discuss the current evidence for structural lung damage associated with A. fumigatus in PwCF on chest computed tomography and magnetic resonance imaging. We conclude that radiological outcomes to identify disease caused by A. fumigatus can be important for clinical studies and management.

show abstract

Section: Interactionsmentioning

confidence: 99%

Aspergillus-related lung disease in people with cystic fibrosis: can imaging help us to diagnose disease?

Elders

Warris

et al. 2021

Eur Respir Rev

View full text Add to dashboard Cite

show abstract

“…BFIs contribute signi cantly to biogeochemical cycles through nutrient and enzyme trade-offs that facilitate biomass recycling [8]. Considering BFIs in health, phenazines secreted from Pseudomonas aeruginosa impair bio lm formation and enhance conidiation in Aspergillus avus, promoting its virulence [9,10]. BFI are important regulators of agriculture, where the plant growth promoting rhizobacteria like Streptomyces and Bacillus selectively promote the ectomycorrhizal fungi while inhibiting the pathogen's growth, and hence modulate the tri-trophic interactions [11].…”

Section: Introductionmentioning

confidence: 99%

Non-Obligate Pairwise Metabolite Cross-Feeding Suggests Ammensalic Interactions Between Bacillus and Aspergillus Species

Singh

Lee

2021

Preprint

View full text Add to dashboard Cite

Metabolite trade-offs at bacterial-fungal interfaces determine their ecological interactions. We designed a non-obligate pairwise metabolite cross-feeding (MCF) between Bacillus and Aspergillus. Cross-feeding Aspergillus metabolites (MCF-1) affected higher growth and biofilm formation in Bacillus. LC-MS-based multivariate analyses (MVA) showed marked variations in the endogenous metabolite profiles between the cross-fed and control Bacillus. We observed and validated that Aspergillus-derived oxylipins were rapidly depleted in Bacillus cultures concomitant with lowered secretion of cyclic lipopeptides (CLPs). Conversely, Bacillus extracts cross-fed to Aspergillus (MCF-2) diminished its mycelial growth and conidiation. Fungistatic effects of Bacillus-derived cyclic surfactins were temporally reduced following their hydrolytic linearization. MVA highlighted disparity between the cross-fed (MCF-2) and control Aspergillus cultures with marked variations in the oxylipin levels. We conclude that the pairwise MCF selectively benefitted Bacillus while suppressing Aspergillus, which suggests their ammensalic interaction. Widening this experimental pipeline across tailored communities may help model and simulate BFIs in more complex microbiomes.

show abstract

“…Concerning Aspergillus spp., the study of its interactions with bacteria is less extended. Indeed, to our knowledge, all the data available on Aspergillus-bacteria interactions almost exclusively concern P. aeruginosa (147,149). Indeed, both A. fumigatus and P. aeruginosa are the most common fungus and bacterium isolated from patients suffering from cystic fibrosis (163), in which they often co-occur as polymicrobial infections (164).…”

Section: Microbiomes and Ecological Medicine -Translating Ecological Concepts Into Human Medicinementioning

confidence: 99%

“…Another study showed that A. fumigatus inhibits biofilm formation in Acinetobacter baumannii via the production of gliotoxin (171). Additionally, Staphylococcus aureus and Streptococcus pneumoniae were shown to inhibit A. fumigatus development (149). Finally, Stenotrophomonas maltophilia was shown to hinder hyphal formation, reduce biofilm formation, and block conidia production in A. fumigatus (149).…”

Section: Microbiomes and Ecological Medicine -Translating Ecological Concepts Into Human Medicinementioning

confidence: 99%

“…Additionally, Staphylococcus aureus and Streptococcus pneumoniae were shown to inhibit A. fumigatus development (149). Finally, Stenotrophomonas maltophilia was shown to hinder hyphal formation, reduce biofilm formation, and block conidia production in A. fumigatus (149).…”

Section: Microbiomes and Ecological Medicine -Translating Ecological Concepts Into Human Medicinementioning

confidence: 99%

See 1 more Smart Citation

Bacterial oxalotrophy as an alternative biocontrol approach for the fight against pulmonary aspergillosis

Palmieri¹

View full text Add to dashboard Cite

Although fungi are estimated to kill more than 1.5 million people every year worldwide, the issue of fungal pathogenesis is largely neglected. Moreover, the rise of emergence of multi-resistant fungal pathogens worldwide is a major threat for human health. This is notably the case of the opportunistic fungal pathogens of the genus Aspergillus. The prevalence of Aspergillus-related infections, also known as aspergillosis, has dramatically increased in the last few years. Aspergillus species, such as A. fumigatus, A. flavus, A. nidulans, A. terreus or A. niger, are known to cause a vast spectrum of respiratory diseases, ranging from mild allergies to life-threatening invasive infections. Interestingly, the formation of calcium oxalate crystals has been previously reported in the latter cases. Oxalic acid is known to play a key role in the pathogenesis of plant fungal pathogens, such as for instance Sclerotinia sclerotiorum. However, a link between the production of oxalic acid and pathogenicity has not been made yet in the case of Aspergillus. Oxalic acid is commonly produced by soil fungi, along with other low molecular weight organic acids. In soils, oxalic acid generally occurs in the form of calcium oxalate crystals. Despite its chemical stability and low solubility, calcium oxalate is rarely found in the geological records, something that has been suggested to be the results of its metabolization by oxalotrophic bacteria. Oxalogenic fungi are known to interact with oxalotrophic bacteria in soils within the oxalate-carbonate pathway, where fungi, along with plants, are the source of oxalate, and oxalotrophic bacteria are its sink. Oxalotrophy is concomitant with a pH increase, which eventually leads to the precipitation of calcium carbonate, if the pH increases above a value of 8.4. The aim of the present thesis was to translate the metabolic interaction between oxalogenic fungi and oxalotrophic bacteria occurring in soils to human health. Specifically, we developed and assessed a novel biocontrol strategy for the treatment of pulmonary aspergillosis based on the manipulation of the environment through bacterial oxalotrophy, a process we named environmental interference. For this, the influence of the composition of the culture medium on the production of low molecular weight organic acids was tested for selected fungal strains, as well as their interaction with non-oxalotrophic and oxalotrophic bacterial strains. The fungal strain Aspergillus niger was selected because of its systematic production of oxalic acid in all culture media tested, and because of its medical relevance. The first demonstration of the principle of environmental interference in-vitro was made by showing the biocontrol exerted by the oxalotrophic bacterial species Cupriavidus oxalaticus on the growth of A. niger. The use of soil bacteria was shown to be problematic, as they induce important cellular damage. Therefore, the genome of C. oxalaticus was analyzed in order to better understand the oxalotrophy metabolism in this model bacterial species. This highlighted the presence of an operon containing all the genes that are required for the degradation of oxalate. These data will be used for the design of an entirely enzymatic degradation pathway of oxalate that will be more suitable as a potential therapeutic option. Finally, the fungal:fungal:bacterial interaction between A. niger, Candida albicans and C. oxalaticus was investigated. This interaction is relevant in the case of pulmonary co-infection in immunocompromised patients and patients suffering from cystic fibrosis. The interaction of both opportunistic fungal pathogens and the oxalotrophic bacterium depended on the inoculation mode (simultaneous versus sequential). To conclude, while the presented results on the biocontrol concept of environmental interference are promising, a preclinical in-vivo demonstration of this concept in a murine infection model is crucial for the development of a potential clinical application. Indeed, a more comprehensive approach by integrating the immune system is necessary in order to better comprehend the interplay between the host, the pathogen and the lung microbiota in disease development.

show abstract

Bacterial Interactions with Aspergillus fumigatus in the Immunocompromised Lung

Cited by 14 publications

References 139 publications

Aspergillus-related lung disease in people with cystic fibrosis: can imaging help us to diagnose disease?

Aspergillus-related lung disease in people with cystic fibrosis: can imaging help us to diagnose disease?

Non-Obligate Pairwise Metabolite Cross-Feeding Suggests Ammensalic Interactions Between Bacillus and Aspergillus Species

Bacterial oxalotrophy as an alternative biocontrol approach for the fight against pulmonary aspergillosis

Contact Info

Product

Resources

About