Bacterial lipopolysaccharide stimulates bovine neutrophil production of TNF-α, IL-1β, IL-12 and IFN-γ

Abstract

-After intramammary infection, polymorphonuclear neutrophil leukocytes (PMN) are the first cells recruited into the mammary gland. Rapid recruitment of and bacterial phagocytosis and killing by PMN are the most effective defenses against establishment of bacterial infection. In addition to their phagocytic and bactericidal properties, PMN may play a key supportive role through secretion of cytokines during the innate immune response. We sought to determine whether bovine PMN produce cytokines in response to stimulation by lipopolysaccharide (LPS). To investigate the effects of LPS on the expression of cytokines secreted by bovine PMN, we measured the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-12, and interferon (IFN)-γ by ELISA after stimulation with different concentrations of LPS, and secretion of IL-8 after co-stimulation with LPS and either TNF-α or IL-1β. Bovine PMN were shown to secrete TNF-α, IL-1β, IL-12, IL-8 and IFN-γ in response to LPS. Co-incubation of PMN with LPS and TNF-α increased secretion of IL-8 when compared to LPS alone. It was concluded that LPS stimulation up-regulates the secretion of cytokines by bovine PMN, and that co-incubation of LPS with TNF-α had an additive effect on the secretion of IL-8. These data show that bovine PMN, in addition to their phagocytic and bactericidal properties, may play a supportive role in the innate immune response to infection by Gram-negative bacteria through their ability to produce immuno-regulating cytokines.lipopolysaccharide / bovine / neutrophils / cytokines / mastitis

Colonic gene expression patterns of mucin muc2 knockout mice reveal various phases in colitis development1

“…[25][26][27] In Muc2 À/À mice, transcription of Timp3, Tnf shedding enzyme inhibitor, was significantly decreased, which implies that the Tnf shedding might be increased in Muc2 À/À mice. Transcription of Tnf receptor Tnfsf1b was also upregulated in Muc2 À/À mice, and high levels of Tnfsf1b can mediate inflammation in a ligand-independent fashion.…”

Protein A‐based antibody immobilization onto polymeric microdevices for enhanced sensitivity of enzyme‐linked immunosorbent assay

“…1). Interferon-g (IFN-g), a pluripotent cytokine with a wide array of biological activities ranging from the modulation of immune responses to the regulation of cell proliferation and apoptosis (Andrianifahanana et al, 2007) was selected as a model drug because it is commonly detected by ELISA (Sohn et al, 2007). For comparison, protein A was also immobilized through conventional GA chemistry.…”

Effect of Mycoplasma bovis on Production of Pro-Inflammatory Cytokines by Peripheral Blood Mononuclear Cells

“…They may play an important role in enhancing the biological response of bovine neutrophils to bacterial infections (Leite et al, 2002;Sohn et al, 2007). Rodriguez et al (2015) conducted immunohisto chemical study for cytokine expression in spontaneously infected animals and found out that M. bovis-associated pneumonic lesions consistently resulted in upregulation of TNF-α, IL-4, IL-10 and IFN-γ expression, but expression of IL-1α, IL-1β, IL-2, IL-6 and IL-8 did not exhibit significant differences between infected and normal lung from control calves.…”