Bacterial Lipopolysaccharides Induce Steroid Sulfatase Expression and Cell Migration through IL-6 Pathway in Human Prostate Cancer Cells

Im, Hee-Jung; Park, Na-Hee; Kwon, Yeo‐Jung; Shin, Sungho; Kim, Donghak; Chun, Young‐Jin

doi:10.4062/biomolther.2012.20.6.556

Cited by 16 publications

(7 citation statements)

References 37 publications

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“…Carcinogenesis was studied in cells treated with LPS, as determined by tumor cell survival, proliferation, invasion and metastasis (1,22,23). LPS induces PC-3 cell migration (24) by stimulating the production of TNF-α and IL-6 (25), and the serum levels of these cytokines are associated with stages of prostate cancer (26). A study investigated the effects of treatment in patients with localized aggressive periodontitis, and observed that improvement in clinical parameters correlated with reductions in responsiveness to LPS, as determined by the release of cytokines, including TNF-α, IL-1β, MCP-1 and IL-6 (27).…”

Section: Discussionmentioning

confidence: 99%

Estrogen receptor β suppresses inflammation and the progression of prostate cancer

et al. 2019

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Previous studies demonstrated that estrogen receptor β (ERβ) signaling alleviates systemic inflammation in animal models, and suggested that ERβ-selective agonists may deactivate microglia and suppress T cell activity via downregulation of nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB). In the present study, the role of ERβ in lipopolysaccharide (LPS)-induced inflammation and association with NF-κB activity were investigated in PC-3 and DU145 prostate cancer cell lines. Cells were treated with LPS to induce inflammation, and ELISA was performed to determine the expression levels of inflammatory cytokines, including tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein 1 (MCP-1), interleukin (IL)-1β and IL-6. MTT and Transwell assays, and Annexin V/propidium iodide staining were conducted to measure cell viability, apoptosis and migration, respectively. Protein expression was determined via western blot analysis. LPS-induced inflammation resulted in elevated expression levels of TNF-α, IL-1β, MCP-1 and IL-6 compared with controls. ERβ overexpression significantly inhibited the LPS-induced production of TNF-α, IL-1β, MCP-1 and IL-6. In addition, the results indicated that ERβ suppressed viability and migration, and induced apoptosis in prostate cancer cells, which was further demonstrated by altered expression of proliferating cell nuclear antigen, B-cell lymphoma 2-associated X protein, caspase-3, E-cadherin and matrix metalloproteinase-2. These effects were reversed by treatment with the ERβ antagonist PHTPP or ERβ-specific short interfering RNA. ERβ overexpression reduced the expression levels of p65 and phosphorylated NF-κB inhibitor α (IκBα), but not total IκBα expression in LPS-treated cells. In conclusion, ERβ suppressed the viability and migration of the PC-3 and DU145 prostate cancer cell lines and induced apoptosis. Furthermore, it reduced inflammation and suppressed the activation of the NF-κB pathway, suggesting that ERβ may serve roles as an anti-inflammatory and anticancer agent in prostate cancer.

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Section: Discussionmentioning

confidence: 99%

Estrogen receptor β suppresses inflammation and the progression of prostate cancer

et al. 2019

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“…20,25 As result of this activation, the levels of pro-inflammatory biomarkers significantly increase in circulation. 26 It is now well known that sustained levels of pro-inflammatory mediators are associated with several pathological conditions including different types of cancer. 27 In the current study, V. iphionoides seems to be able to protect MRC-5 against a dramatic increase of IL-6 levels due to their exposure to the bacterial endotoxin LPS (500 ng/mL) (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effect of Varthemia iphionoides extracts against prostate cancer in vitro

et al. 2017

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Varthemia iphionoides is a Jordanian medicinal plant with several health-promoting properties, including antibacterial, antioxidant and anticancer activities. However, its anti-inflammatory properties have been poorly investigated up to date. The current study aimed to investigate the anti-inflammatory effect of V. iphionoides by measuring the production of interleukin-6 in response to a pro-inflammatory stimulus (bacterial lipopolysaccharide) in in vitro cell models of human MRC-5 and PC3 cells. We observed a significant reduction in lipopolysaccharide-induced interleukin-6 release in response to V. iphionoides (125 µg/mL) in both non-cancerous fibroblast MRC-5 and prostate cancerous PC3 cells. However, the anti-inflammatory effect of this medicinal plant was stronger when MRC-5 cells were treated with an aqueous extract, while the methanolic extract was more potent in PC3 cells. The effect of V. iphionoides in reducing interleukin-6 production was not due to its cytotoxicity, and future studies are required to elucidate the mechanisms of action by which this medicinal plant modulates inflammatory responses. In conclusion, the results of our study represent the first report of the potential protective effect of water and methanolic extracts of V. iphionoides against pro-inflammatory stimuli in fibroblasts and cancer cells of human origin, and it is critically important to identify the phytochemical compounds responsible for this effect.

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“…In this study, the effect of LPS on skin fibroblasts was investigated. LPS is the main component of Gram-negative bacteria [15]. In this study, LPS inhibited the growth of human skin fibroblasts and was associated with impaired function of endoplasmic reticulum and expression of markers of apoptosis, which occurs in vivo to remove damaged cells.…”

Section: Discussionmentioning

confidence: 75%

microRNA-211-3p has a Role in the Effects of Lipopolysaccharide on Endoplasmic Reticulum Stress in Cultured Human Skin Fibroblasts

Wang

2019

Med Sci Monit Basic Res

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Background Lipopolysaccharide (LPS) in bacterial infection of skin wounds delays wound healing. This study aimed to investigate the effects of LPS and endoplasmic reticulum stress in cultured skin fibroblasts and microRNA-211-3p (miR-211-3p) signaling. Material/Methods Human skin fibroblasts were cultured in increasing concentrations of LPS at 0 ng/ml, 5 ng/ml, 10 ng/ml, and 20 ng/ml for 0, 12 h, 24 h, 36 h, and 48 h. Cell proliferation was determined using the MTT assay. Protein expression levels of the transcription factors GRP78, CHOP, p-JNK, and the endoplasmic reticulum stress apoptosis proteins, caspase-12 and Bcl-2, were determined by Western blot. The expression of miR-211-3p in human skin fibroblasts was detected by quantitative polymerase chain reaction (qPCR). Results Cell proliferation of human skin fibroblasts decreased with increasing concentrations of LPS in a dose-dependent and time-dependent way. Protein levels of GRP78, CHOP, p-JNK, caspase-12, and Bcl-2 were increased 8 h and 12 h after LPS treatment compared with 0 h and 4 h after treatment. However, the expression of miR-211-3p was decreased in human skin fibroblasts after treatment with LPS. When miR-211-3p was overexpressed, the endoplasmic reticulum stress/CHOP related proteins, including GRP78, CHOP, p-JNK, caspase-12, and Bcl-2 were unchanged after the addition of LPS. Overexpression of miR-211-3p also reduced inhibitory effects of LPS on the growth of human skin fibroblasts. Conclusions This study showed that microRNA-211-3p had a role in the effects of LPS on endoplasmic reticulum stress and CHOP activation in cultured human skin fibroblasts.

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Bacterial Lipopolysaccharides Induce Steroid Sulfatase Expression and Cell Migration through IL-6 Pathway in Human Prostate Cancer Cells

Cited by 16 publications

References 37 publications

Estrogen receptor β suppresses inflammation and the progression of prostate cancer

Estrogen receptor β suppresses inflammation and the progression of prostate cancer

Anti-inflammatory effect of Varthemia iphionoides extracts against prostate cancer in vitro

microRNA-211-3p has a Role in the Effects of Lipopolysaccharide on Endoplasmic Reticulum Stress in Cultured Human Skin Fibroblasts

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