Bacterial membrane transporter systems for aromatic compounds: Regulation, engineering, and biotechnological applications

Mutanda, Ishmael; Sun, Jianzhong; Jiang, Jianxiong; Zhu, Daochen

doi:10.1016/j.biotechadv.2022.107952

Cited by 39 publications

(16 citation statements)

References 170 publications

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“…According to previous reports, , efficient transporters could significantly increase the product yield. In the production of NMN, Shoji et al introduced transporter PnuC from Bacillus mycoides to E.…”

Section: Resultsmentioning

confidence: 99%

Metabolic Engineering of Bacillus licheniformis for the Bioproduction of Nicotinamide Riboside from Nicotinamide and Glucose

Zhou

Che

et al. 2023

ACS Sustainable Chem. Eng.

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Nicotinamide riboside (NR) is an important nucleotide, a precursor of nicotinamide adenine dinucleotide, that has attracted great attention as a promising nutraceutical to improve various symptoms of diabetes, vascular disease, and age-related physiological decline. Here, we systematically engineered Bacillus licheniformis to produce NR from glucose and nicotinamide (NAM) efficiently. Specifically, we selected the pathway using nicotinamide phosphoribosyltransferase (NAMPT) for converting NAM to nicotinamide mononucleotide (NMN) in the presence of 5-phosphoribosyl-1-pyrophosphate (PRPP) and increased the titer of NMN by engineering NAMPT and strengthening the PRPP supply. To prevent the degradation of NR, genes deoD and pupG coding purine nucleoside phosphorylase were deleted, resulting in 1.76 g/L NR accumulation in the culture medium. Then, we further deleted the nicotinamidase PncA encoding gene to prevent the synthesis of the byproduct nicotinate and overexpressed nucleotidase YfkN during the conversion of NMN to NR, where the titer of NR was increased by 226 and 41%, respectively. Notably, the efflux pump MdtL from Escherichia coli was first proven to be beneficial for NR production. Finally, combined with process optimization, the recombinant strain NR17 produced 11.33 g/L NR with a yield of 0.91 mol/mol NAM and a productivity of 0.44 g/(L•h) in a shake flask. An NR producer was constructed in this study and will be promising for the low-cost, high-quality industrial production of NR.

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Section: Resultsmentioning

confidence: 99%

Metabolic Engineering of Bacillus licheniformis for the Bioproduction of Nicotinamide Riboside from Nicotinamide and Glucose

Zhou

Che

et al. 2023

ACS Sustainable Chem. Eng.

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“…In general terms, the known molecular mechanisms for xenobiotic degradation include recognition and uptake, activation of catabolic genes/enzymes, and extrusion [ 109 , 110 ]. The initial recognition and uptake of these compounds occurs both via passive diffusion or active transport (e.g., major facilitator superfamily transporters, outer membrane porins), which are part of the molecular stress response mechanisms of bacteria [ 65 , 111 , 112 ]. Once inside the cell, catabolic genes are induced and xenobiotics undergo transformation by peripheral enzymes (e.g., monooxygenases, dioxygenases, nitroreductases, laccases, dehalogenases, peroxidases, etc.)…”

Section: Resultsmentioning

confidence: 99%

“…When xenobiotics are not metabolized, they can also be directly extruded by efflux pumps (e.g., multidrug and toxic compound extrusion (MATE) family, Mex-type multidrug resistance efflux pumps, ATP-binding cassette transporters, etc.) as a defense mechanism that prevents cytotoxic concentrations from being reached [ 65 , 111 ].…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation

Cabrera

Márquez

Pérez-Donoso

2022

Genes

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The nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) is a highly toxic and persistent environmental pollutant. Since physicochemical methods for remediation are poorly effective, the use of microorganisms has gained interest as an alternative to restore TNT-contaminated sites. We previously demonstrated the high TNT-transforming capability of three novel Pseudomonas spp. isolated from Deception Island, Antarctica, which exceeded that of the well-characterized TNT-degrading bacterium Pseudomonas putida KT2440. In this study, a comparative genomic analysis was performed to search for the metabolic functions encoded in the genomes of these isolates that might explain their TNT-transforming phenotype, and also to look for differences with 21 other selected pseudomonads, including xenobiotics-degrading species. Comparative analysis of xenobiotic degradation pathways revealed that our isolates have the highest abundance of key enzymes related to the degradation of fluorobenzoate, TNT, and bisphenol A. Further comparisons considering only TNT-transforming pseudomonads revealed the presence of unique genes in these isolates that would likely participate directly in TNT-transformation, and others involved in the β-ketoadipate pathway for aromatic compound degradation. Lastly, the phylogenomic analysis suggested that these Antarctic isolates likely represent novel species of the genus Pseudomonas, which emphasizes their relevance as potential agents for the bioremediation of TNT and other xenobiotics.

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“…On the other hand, a small part of the intracellular NMN will be consumed to synthesize NAD + for bacterial growth, which is not beneficial for the accumulation of NMN. According to previous reports, 33,34 efficient transporters could efficiently increase product biosynthesis. The diffusion of a major portion of NMN outside of cells (60.4%) by the VpNadV-overexpressing strain showed that there could be some transporters in E. coli that transport the secreted NMN.…”

Section: Enhanced Nmn Production Following the Introduction Of Bmpnucmentioning

confidence: 88%

Systematic Engineering of Escherichia coli for Efficient Production of Nicotinamide Mononucleotide From Nicotinamide

Huang

et al. 2022

ACS Synth. Biol.

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Research studies on NAD + have proven its crucial role in aging and disease. Nicotinamide mononucleotide (NMN), as the key intermediate of NAD + , plays a significant role in supplying and maintaining NAD + levels. In the present study, a biocatalytic method for the efficient synthesis of NMN was established. First, Escherichia coli was systematically modified to make it more conducive to the biosynthesis and accumulation of NMN. Next, the performance of nicotinamide phosphoribosyltransferase from Vibrio bacteriophage KVP40 (VpNadV) was determined, which has the best catalytic activity to produce NMN from nicotinamide. The accumulation of extracellular NMN was further increased after the introduction of an NMN transporter. Fine-tuning of gene expression and copy number led to the synthesis of NMN at the yield of 2.6 g/L at the shake flask level. The introduction of a nicotinamide transporter, BcniaP, could not obviously increase the production of NMN at the shake flask level, but it decreased the production of NMN at the bioreactor level. Finally, the titer of NMN reached 16.2 g/L with a conversion ratio of 97.0% from nicotinamide, both of which are highest according to currently available reports. The fed-batch fermentation with direct supplementation of nicotinamide could facilitate the industrialscale production of NMN compared to that achieved by the whole-cell catalysis process. These results also represent the highest reported yield of NMN synthesized from nicotinamide in E. coli.

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Bacterial membrane transporter systems for aromatic compounds: Regulation, engineering, and biotechnological applications

Cited by 39 publications

References 170 publications

Metabolic Engineering of Bacillus licheniformis for the Bioproduction of Nicotinamide Riboside from Nicotinamide and Glucose

Metabolic Engineering of Bacillus licheniformis for the Bioproduction of Nicotinamide Riboside from Nicotinamide and Glucose

Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation

Systematic Engineering of Escherichia coli for Efficient Production of Nicotinamide Mononucleotide From Nicotinamide

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