Bacterial penetration through temporary restorative materials in root‐canal‐treated teeth <i>in vitro</i>

Imura, Noboru; Otani, Sonia Mic; Avila-Campos, Mario Júlio; Jardim, Edymar; Zuolo, Mário Luis

doi:10.1046/j.1365-2591.1997.00100.x

Cited by 18 publications

(32 citation statements)

References 18 publications

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“…The duration of 40 days for this study was based on the literature 2,7,8,10 . The group of opened and instrumented root canals used as a positive group (PC1) was penetrated by human saliva microorganisms during a period of 24 h and, as expected, proved that root canals exposed to the oral cavity are infected in a short period.…”

Section: Discussionmentioning

confidence: 99%

“…The use of human saliva has an advantage because it is faithfully closer to the real clinical situation. However, it does not simulate similar variables that exist in the buccal environment given temperature changes, the influence of the diet, and salivary flow 8 . Further studies are necessary to investigate the relationship between the leakage of root fillings and the reactions of periradicular tissues 15 .…”

Section: Discussionmentioning

confidence: 99%

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Coronal microleakage of endodontically treated teeth with intracanal post exposed to fresh human saliva

Oliveira

Gomes²,

Costa³

et al. 2013

J. Appl. Oral Sci.

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ObjectiveThe aim of this study was to investigate the coronal microleakage of endodontically treated teeth prepared to receive an intracanal post and teeth with an intracanal post but without a prosthetic crown and exposed to contamination by fresh human saliva.Material and MethodsA mechanical-chemical preparation following the step-back technique was carried out in 35 extracted single-rooted human teeth. The teeth were randomly divided into five groups: G1=root canals instrumented, obturated, and prepared to receive an intracanal post (N=10); G2=root canals with cemented posts but without coronal sealing (N=10); PC1=positive control root canals instrumented and open (N=5); PC2=positive control 2 root canals without instrumentation and open (N=5); and NC=negative control healthy teeth (N=5). The crowns were removed except for the control group of intact teeth. The root canals were obturated and sterilized with cobalt 60 gamma irradiation and were then adapted in an apparatus using a Brain Heart Infusion (BHI) medium and fresh human saliva for contamination. Microbial growth was indicated by the presence of turbidity in the BHI liquid medium.ResultsData were submitted to the Kaplan-Meier Survival Analysis and the Holm-Sidak statistic method, which observed an index of 90% of microleakage in root canals after 24 hours for G1 and 70% of microleakage in samples at the end of 40 days for G2.ConclusionThe results show that root canals with an intracanal post but without a prosthetic crown can be recontaminated when exposed to fresh human saliva in a short period.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Coronal microleakage of endodontically treated teeth with intracanal post exposed to fresh human saliva

Oliveira

Gomes²,

Costa³

et al. 2013

J. Appl. Oral Sci.

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“…This methodology is reproducible and has clinical relevance, presenting reliable data and simulating clinical conditions 7,13,16 . This methodology allows the observation of the exact day of the sample contamination, showed by the broth turbidity.…”

Section: Discussionmentioning

confidence: 98%

A microleakage study of gutta-percha/AH Plus and Resilon/Real self-etch systems after different irrigation protocols

2014

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The development and maintenance of the sealing of the root canal system is the key to the success of root canal treatment. The resin-based adhesive material has the potential to reduce the microleakage of the root canal because of its adhesive properties and penetration into dentinal walls. Moreover, the irrigation protocols may have an influence on the adhesiveness of resin-based sealers to root dentin.ObjectiveThe objective of the present study was to evaluate the effect of different irrigant protocols on coronal bacterial microleakage of gutta-percha/AH Plus and Resilon/Real Seal Self-etch systems.Material and MethodsOne hundred ninety pre-molars were used. The teeth were divided into 18 experimental groups according to the irrigation protocols and filling materials used. The protocols used were: distilled water; sodium hypochlorite (NaOCl)+eDTA; NaOCl+H3PO4; NaOCl+eDTA+chlorhexidine (CHX); NaOCl+H3PO4+CHX; CHX+eDTA; CHX+ H3PO4; CHX+eDTA+CHX and CHX+H3PO4+CHX. Gutta-percha/AH Plus or Resilon/Real Seal Se were used as root-filling materials. The coronal microleakage was evaluated for 90 days against Enterococcus faecalis. Data were statistically analyzed using Kaplan-Meier survival test, Kruskal-Wallis and Mann-Whitney tests.ResultsNo significant difference was verified in the groups using chlorhexidine or sodium hypochlorite during the chemo-mechanical preparation followed by eDTA or phosphoric acid for smear layer removal. The same results were found for filling materials. However, the statistical analyses revealed that a final flush with 2% chlorhexidine reduced significantly the coronal microleakage.ConclusionA final flush with 2% chlorhexidine after smear layer removal reduces coronal microleakage of teeth filled with gutta-percha/AH Plus or Resilon/Real Seal SE.

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“…The microbiological test consisted of a 2-chamber method described by Imura et al (16) which we adapted for the dental crowns of our study (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

Ex vivo study of bacterial coronal leakage in indirect pulp treatment

Ruiz¹,

Baca²,

Pardo-Ridao³

et al. 2013

Med Oral

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Objective: The aim of this study was to evaluate, ex vivo, bacterial coronal leakage with different antimicrobial agents applied to the dentine for indirect pulp treatment (IPT). Study Design: Sixty extracted teeth were prepared and randomly distributed into 5 groups (n=10): Group 1: no antimicrobial dentine treatment; group 2: 1% chlorhexidine (CHX)+1% thymol varnish (Cervitec®); group 3: 2 % CHX solution; group 4: 40% CHX varnish (EC40™) and group 5: Clearfil™ Protect Bond (CPB). Ten teeth served as controls. The teeth were restored using a resin-modified glass ionomer cement (GIC) and then mounted in a two-chamber device. The coronal access was exposed to Streptococcus mutans for 45 days. The appearance of turbidity in the BHI broth of the lower chamber was considered as specimen leakage. Results: Survival analysis, determined by non parametric Kaplan-Meier and log-rank tests, showed that the best results were for groups EC40™+GIC and GIC alone; yet there were not statistically significant differences between them. All specimens of CPB+GIC and 2% CHX+GIC, leaked at 45 days. Conclusions: In IPT the use of GIC without pretreatment of the dentine and pretreatment with 40% CHX varnish resulted in a significant delay of bacterial coronal leakage. Key words:Streptococcus mutans, bacterial leakage, resin-modified glass ionomer cement, indirect pulp treatment.

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Bacterial penetration through temporary restorative materials in root‐canal‐treated teeth in vitro

Cited by 18 publications

References 18 publications

Coronal microleakage of endodontically treated teeth with intracanal post exposed to fresh human saliva

Coronal microleakage of endodontically treated teeth with intracanal post exposed to fresh human saliva

A microleakage study of gutta-percha/AH Plus and Resilon/Real self-etch systems after different irrigation protocols

Ex vivo study of bacterial coronal leakage in indirect pulp treatment

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