Bacterial Surface Display of Levansucrase of Zymomonas mobilis Using Bacillus Subtilis Spore Display System

김준형,; 최수근,; 정흥채,; 반재구,; 김병기,

doi:10.7841/ksbbj.2011.26.3.243

Cited by 1 publication

(2 citation statements)

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“…The pCSK1 vector was derived from pHP59, which is a low copy E. coli/B. subtilis shuttle vector described by [32].…”

Section: Construction Of Fusion Protein Expression Vectormentioning

confidence: 99%

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Decolorization of Acid Green 25 by Surface Display of CotA laccase on Bacillus subtilis spores

Park¹,

Kim²,

Lee³

et al. 2019

Journal of Microbiology and Biotechnology

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Introduction Approximately 10,000 different dyes and pigments are used industrially, and over 7 × 10 5 tons of synthetic dyes are produced annually worldwide [1, 2]. Synthetic dyes are extensively used in textile dyeing, paper printing, color photography, pharmaceutical, food, cosmetics, and other industries [3]. Approximately 128 tons of dye wastewater is discharged daily and high cost is incurred for its removal. Wastewater remediation is challenging because it contains a variety of pollutants and it is difficult to change the composition of the contaminants. Physical, chemical, and biological methods have been widely used to decolorize dye wastewater [4]. The physical and chemical treatment methods are preferred over other methods; however, these methods require high operating cost and other accessory equipment. It would be desirable to develop a new decolorization method that is efficient and cost effective to reduce the overall cost of dye wastewater treatment. Recently, many studies have been published on the decolorization of synthetic dyes by laccase [5, 6]. Laccase was first discovered in 1883 from Rhus vernicifera, the Japanese lacquer tree [7]. Laccases (benzenediol: oxygen oxidoreductase; E.C. 1.10.3.2) are a family of multi-copper oxidases (MCOs) that require oxygen to oxidize organic compounds-particularly phenols and non-phenolic aromatic substrates-by one-electron transfer to form reactive radicals,

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“…The pCSK1 vector was derived from pHP59, which is a low copy E. coli/B. subtilis shuttle vector described by [32].…”

Section: Construction Of Fusion Protein Expression Vectormentioning

confidence: 99%

“…The washed spores were resuspended in 1 ml PBS solution containing anti-His 6 tag-FITC labeled antibody (1:500) (Komabiotech, Korea) and incubated for 1 h on ice. Thereafter, they were washed three times with PBS, resuspended in 500 μl PBS [32], and examined under a flow cytometer (Beckman Coulter Epics, UK).…”

Section: Flow Cytometry Analysismentioning

confidence: 99%