Bacterial Uropathogens in Urinary Tract Infection and Antibiotic Susceptibility Pattern in Riyadh Hospital, Saudi Arabia

Al-Mijalli, Samiah Hs

doi:10.21767/2573-5365.100028

Cited by 13 publications

(20 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the 100 urine samples collected in this study, 76 (76%) belonged to female population while 24 (24%) belonged to male patients. The gender distribution of patients in our study is consistent with many previous local [ 35 , 36 ] and international [ 37 , 38 ] reports showing the high incidence of UTI in women compared to men across all age groups. It has been demonstrated that the high UTI incidence in women can be attributed to many reasons such as anatomical factors that allow quick access of bacteria to the bladder, poor hygiene, sexual activity, and use of contraceptives [ 39 ].…”

Section: Discussionsupporting

confidence: 91%

Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia

Alqasim

Jaffal

Alyousef

2018

International Journal of Microbiology

View full text Add to dashboard Cite

The prevalence of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-producing E. coli) has recently increased worldwide. This study aims at determining the antimicrobial susceptibility patterns of a collection of clinical E. coli urine isolates and evaluating the ESBL carriage of these isolates at phenotypic and genotypic levels. A total of 100 E. coli urine isolates were collected at a tertiary healthcare centre in Riyadh from January 2018 to March 2018. Antimicrobial susceptibility testing was carried out for all isolates. ESBL production was characterized at phenotypic and genotypic levels using double-disc synergy test and polymerase chain reaction, respectively. Detection of different ESBL variants was performed using DNA sequencing. Of 100 E. coli isolates, 67 were associated with multidrug resistance (MDR) phenotype. All isolates showed variable resistance levels to all antibiotics used here expect to imipenem, where they were all imipenem-sensitive. 33 out of 100 E. coli isolates were positive for ESBLs by phenotypic and genotypic methods. Among all ESBL-positive E. coli isolates, the CTX-M was the most prevalent ESBL type (31/33 isolates; 93.94%). CTX-M-15 variant was detected in all isolates associated with CTX-M carriage. Multiple ESBL gene carriage was detected in 15/33 isolates (45.45%), where 11 (33.33%) isolates produced two different ESBL types while 4 isolates (12.12%) associated with carrying three different ESBL types. Our study documented the high antimicrobial resistance of ESBL-producing E. coli to many front-line antibiotics currently used to treat UTI patients, and this implies the need to continuously revise the local guidelines used for optimal empirical therapy for UTI patients. It also showed the high prevalence of ESBL carriage in E. coli urine isolates, with CTX-M-15 being the most predominant CTX-M variant.

show abstract

Section: Discussionsupporting

confidence: 91%

Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia

Alqasim

Jaffal

Alyousef

2018

International Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…Recently, the level of ExPEC resistance to cephalosporins has increased markedly. For example, Al-Mijalli has reported that about 99 % of the all tested E. coli isolates were resistant to cefalotin, ceftriaxone, cefuroxime and ceftazidime, while resistance to cefoxitin and cefepime was reported in 14 and 96.7 % of isolates, respectively [14]. Another report has demonstrated that ESBL-producing UPEC isolates were associated with complete resistance to cefotaxime, ceftazidime and cefepime, and it also showed that 70.3% of these isolates were cefoxitin-resistant [9] (Table 1).…”

Section: Cephalosporinsmentioning

confidence: 99%

“…However, the level of ExPEC resistance to aztreonam has recently increased. For instance, it has been found that 74.4% of ESBL-producing UPEC isolates were aztreonam-resistant [14] ( Table 1). Another study has demonstrated that all tested ESBL-producing UPEC isolates were aztreonam-resistant, while aztreonam resistance remained very low among non-ESBL-producing ExPEC isolates and it was only detected in 1.65% of the tested isolates [9].…”

Section: Monobactamsmentioning

confidence: 99%

“…Over the past decade, E. coli resistance to carbapenems, such as imipenem and meropenem, has been uncommon, and the range of resistance was 0 -8.5 % for imipenem and 0 -1.1 % for meropenem [6,14,15].…”

Section: Carbapenemsmentioning

confidence: 99%

“…Norfloxacin resistance of ExPEC has also recently increased, and this resistance has been reported in as many as 44% of ExPEC isolates [10]. ExPEC resistance to levofloxacin has also been high, ranging between 28 and 63 % [8,11,14] (Table 1).…”

Section: Resistance To Fluoroquinolonesmentioning

confidence: 99%

See 2 more Smart Citations

Extraintestinal pathogenic <i>Escherichia coli</i> in Saudi Arabia: A review of antimicrobial resistance and molecular epidemiology

Alqasim¹

2020

Trop. J. Pharm Res

View full text Add to dashboard Cite

Extra-intestinal pathogenic Escherichia coli (ExPEC) is commonly associated with causing urinary tract and bloodstream infections. Over the past two decades, the antimicrobial resistance of ExPEC has increasingly been reported [1]. Given that Saudi Arabia annually hosts mass religious events, such as Hajj, this review investigated several aspects of antimicrobial resistance of ExPEC in this country including the current prevalence of resistance and molecular epidemiology of ExPEC isolates. Generally, the overall prevalence of antibiotic resistance of ExPEC in Saudi Arabia is on increase. The current emergence of colistin resistance in ExPEC represents a major challenge to public health. Local molecular epidemiological studies have shown the dominance of E. coli sequence type 131 (E. coli ST131) over other major ExPEC STs. This is an important observation given that this clone has been associated with high multidrug resistance and extended-spectrum β-lactamases carriage. To reduce the burden of this resistance in the future, it would be crucial to avoid uncontrolled use of antibiotics in either clinical settings or animal food industry. Keywords: Extra-intestinal pathogenic Escherichia coli, Antimicrobial resistance, ST131, Saudi Arabia, Colistin resistance, Extended-spectrum β-lactamases

show abstract

Antimicrobial Resistance Patterns, Extended-Spectrum Beta-Lactamase Production, and Associated Risk Factors of Klebsiella Species among UTI-Suspected Patients at Bahir Dar City, Northwest Ethiopia

Ameshe¹,

Engda

Gizachew

2022

International Journal of Microbiology

View full text Add to dashboard Cite

Introduction. Klebsiella species cause pneumonia, UTI, and septicemia in human beings. Beta-lactam drugs are used extensively to treat patients infected with Klebsiella, but most of the Klebsiella species are resistant to third- and fourth-generation cephalosporins and monobactams to which data are scarce in the study area. Objective. To determine the prevalence, antimicrobial resistance, ESBL production, and associated risk factors of Klebsiella species among UTI-suspected patients in Bahir Dar City, Northwest Ethiopia. Methods. A multi-institution-based prospective cross-sectional study was conducted from January to May 2019. Midstream urines were collected from 385 patients and inoculated onto CLED and MacConkey agars. Identification of growth was done by a battery of biochemical tests. Antimicrobial resistance and ESBL production patterns were determined by using the disc-diffusion method on Mueller–Hinton agar. Quality of data was maintained by following SOPs and using Klebsiella pneumoniae (ACTT700603). Logistic regression statistical analysis was done using the SPSS, version 25, statistical package. A p value ≤ 0.05 was considered statistically significant. Results. The median age of the study participants was 32 years. Majority of them were female, urban residents, and unable to read and/or write. The total Klebsiella species detected were 38 (9.9%). Of which, 25 (65.8%) were Klebsiella pneumoniae, followed by 6 (15.8%) Klebsiella ozaenae. 20 (80%), 19 (76%), and 19 (76%) Klebsiella pneumoniae showed resistance to amoxicillin/clavulanic acid, ampicillin, and cotrimoxazole, respectively. All K. oxytoca were resistant to ampicillin, and all K. rhinoscleromatis were resistant to cefoxitin and cefotaxime. Klebsiella species that showed resistance to ≥3 antimicrobials were 26 (68%). ESBL-producing Klebsiella species were 10 (26.3%). Patients who had history of antibiotic use, hospitalization, and tight dressing habit had more risk of getting UTI ( p < 0.05 ) than their counterparts. Conclusions. Overall UTI prevalence in our study was lower than that of previous Ethiopian studies. High MDR and ESBL-producing Klebsiella species were detected. Patients’ history of antibiotic use, hospitalization, and tight dressing habit were risk factors for UTI. It calls up for improving prevention/control systems of Klebsiella species.

show abstract

Bacterial Uropathogens in Urinary Tract Infection and Antibiotic Susceptibility Pattern in Riyadh Hospital, Saudi Arabia

Abstract: Background: Urinary tract infection (UTI) is one of the commonest infections encountered by clinicians and despite the widespread availability of antimicrobial agents. UTI has become difficult to treat because of appearance of pathogens with increasing resistance to antimicrobial agents.

Cited by 13 publications

References 28 publications

Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia

Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia

Extraintestinal pathogenic <i>Escherichia coli</i> in Saudi Arabia: A review of antimicrobial resistance and molecular epidemiology

Antimicrobial Resistance Patterns, Extended-Spectrum Beta-Lactamase Production, and Associated Risk Factors of Klebsiella Species among UTI-Suspected Patients at Bahir Dar City, Northwest Ethiopia

Contact Info

Product

Resources

About