Bactericidal Activity of a Monoclonal Antibody Against a Recombinant 40‐kDa Outer Membrane Protein of <i>Porphyromonas gingivalis</i>

Katoh, Mitsunobu; Saito, Shigeno; Takiguchi, Hisashi; Abiko, Yoshimitsu

doi:10.1902/jop.2000.71.3.368

Cited by 33 publications

(18 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the role of Fc␣RI and Fc␥RI in the pathogenesis of periodontitis remains unclear. As an approach to enhancing anti-P. gingivalis PMN function or to inhibiting colonization, major attention has been focused on local passive immunization with polyclonal antibodies or monoclonal antibodies (MAb) (3,28,47). It is therefore important to clarify the relative contributions of IgG and IgA receptors in triggering the anti-P. gingivalis function of GCF PMN.…”

mentioning

confidence: 99%

Effective In Vitro Clearance ofPorphyromonas gingivalisby Fcα Receptor I (CD89) on Gingival Crevicular Neutrophils

et al. 2001

View full text Add to dashboard Cite

Porphyromonas gingivalis has been implicated as a causative pathogen in periodontitis. Immunotherapeutic approaches have recently been suggested to aid in the clearance of P. gingivalis from disease sites. Because antibody-Fc receptor (FcR) interactions play a role in the effector functions of polymorphonuclear neutrophils (PMN), we evaluated which FcR on PMN from gingival crevicular fluid (GCF) serves as an optimal target molecule for FcR-directed immunotherapy. GCF PMN and peripheral blood (PB) PMN from adult periodontitis patients were analyzed for their immunoglobulin G (IgG) and IgA FcR (Fc␥R and Fc␣R, respectively) expression and function by studying IgG-and IgA-mediated elimination of P. gingivalis. GCF PMN exhibited higher Fc␣RI and Fc␥RI levels and lower Fc␥RIIa and Fc␥RIIIb levels than PB PMN. Functional studies revealed that GCF PMN exhibited less of a capacity to phagocytose and kill IgG1-opsonized P. gingivalis than PB PMN. IgA1-mediated phagocytosis and killing capacity was, however, comparable between GCF PMN and PB PMN. In summary, these in vitro results document that Fc␣RI represents a candidate target for FcRdirected immunotherapy for the clearance of P. gingivalis.

show abstract

mentioning

confidence: 99%

Effective In Vitro Clearance ofPorphyromonas gingivalisby Fcα Receptor I (CD89) on Gingival Crevicular Neutrophils

et al. 2001

View full text Add to dashboard Cite

show abstract

“…In this regard, it has been shown that 40k-OMP-specific rabbit monoclonal antibodies inhibit coaggregation activity of P. gingivalis (14,16,20). Further, these monoclonal antibodies possess complement-mediated bactericidal activity to P. gingivalis (17,18). Moreover, a recent study has demonstrated that these antibodies have opsonic activity on human neutrophil function for phagocytosis of P. gingivalis (19).…”

Section: Discussionmentioning

confidence: 99%

“…Further, these monoclonal antibodies possess a complement-mediated bactericidal activity to P gingivalis (17,18). It has also been demonstrated that anti40k-OMP antibody opsonizes P gingivalis as a target for phagocytosis by the human neutrophil cell line (19).…”

Section: Introductionmentioning

confidence: 99%

Nasal Immunization with P. gingivalis Surface Protein Antigen and Cholera Toxin Adjuvant Induces T Helper 2 Responses in Both Mucosal and Systemic Compartments

et al. 2003

Self Cite

View full text Add to dashboard Cite

It is well establishedthat Porphyromonas gingivalisis one of the major pathogens of adult periodontitis. P. gingivalis produces an outer membrane protein with a molecular mass of 40-kDa (40k-OMP). In the present study, in order to assess the potential for application of 40k-OMP in the development of an antiperiodontal disease vaccine, we analyzed 40k-OMP-specific CD4+ T helper (Th) cell responses induced in the mucosal as well as systemic compartments when 40k-OMP was administered nasally. When CD4+T cells that were isolated from cervical lymph nodes (CLN) and spleens of mice immunized with 40k-OMP plus cholera toxin (CT) as adjuvant were re-stimulated with 40k-OMP in vitro, significant levels of proliferative responses were induced. In contrast, essentially no increased proliferation occurred in CLN and spleens taken from mice given 40k-OMP alone. Analysis of T helper (Th) 1 (IFN-y) and Th2 (IL-4, IL-5 and IL-6) cytokine responses showed that 40k-OMP-specific Th cells from both CLN and the spleen produced significant levels of IL-4, IL-5 and IL-6 but did not result in changes in IFN-y production. In contrast, marginal levels of IL-4, IL-5 and IL-6 production were seen in mice given 40k-OMP alone nasally. These results suggest that nasal administration of 40k-OMP plus CT as an adjuvant can elicit 40k-OMP-specific Th2-type cytokine responses in both mucosal and systemic compartments. Further, the nasal 40k-OMP vaccine possesses the potential for antiperiodontal vaccine.

show abstract

“…Polyclonal anti-40 kDa.. OMP antibody exhibited potentially protective, complement-mediated bactericidal effect. 12 Gingipains [13], [14] Coined by Travis and Colleague These are cysteine proteinases which cleave synthetic and natural substrates after arginine or lysine residues and are referred to as arginine gingipain (Rgp) and lysine gingipain (Kgp), respectively.…”

Section: Outer Membrane Proteinmentioning

confidence: 99%

Periodontal Vaccine—A Boon In Periodontics

Kumar¹,

B²,

Dutt³

et al. 2014

IOSRJDMS

View full text Add to dashboard Cite

Bactericidal Activity of a Monoclonal Antibody Against a Recombinant 40‐kDa Outer Membrane Protein of Porphyromonas gingivalis

Abstract: Pg-ompA2 has an in vitro complement-mediated bactericidal activity to P. gingivalis. Pg-ompA2 may contribute to the development of a local immunotherapy that can be applied in the gingival crevice of a patient with P. gingivalis-related periodontitis, or be a vaccine candidate.

Cited by 33 publications

References 40 publications

Effective In Vitro Clearance ofPorphyromonas gingivalisby Fcα Receptor I (CD89) on Gingival Crevicular Neutrophils

Effective In Vitro Clearance ofPorphyromonas gingivalisby Fcα Receptor I (CD89) on Gingival Crevicular Neutrophils

Nasal Immunization with P. gingivalis Surface Protein Antigen and Cholera Toxin Adjuvant Induces T Helper 2 Responses in Both Mucosal and Systemic Compartments

Periodontal Vaccine—A Boon In Periodontics

Contact Info

Product

Resources

About