Bactericidal Activity of Catecholamine Copper Complexes

Copper as a Biocidal Tool

“…The cells were partially protected by superoxide dismutases (SOD) or catalase but not by OH radical scavengers. The addition of H 2 O 2 to cells, which were sensitized by preincubation with the epinephrine-copper complex, caused rapid killing and DNA degradation [122].…”

Bactericidal activity of catechin-copper (II) complexes against Staphylococcus aureus compared with Escherichia coli

“…The culture was grown for at least three generations to late logarithmic phase (0·8 O.D. at 600 nm) ( Aronovitch et al . 1991 ).…”

“…The culture was sedimented by centrifugation, washed twice in a buffer [1 mmol l −1 phosphate buffer (pH 7·4) and 1 mmol l −1 MgSO 4 ], and resuspended in a reaction buffer [10 mmol l −1 2‐( N ‐morpholino)ethanesulphonic acid (MES, pH 6·5) and 1 mmol l −1 MgSO 4 ] to 2–4 × 10 7 cells ml −1 ( Aronovitch et al . 1991 ).…”

Bactericidal activity of catechin-copper (II) complexes on Escherichia coli ATCC11775 in the absence of hydrogen peroxide

“…The culture was grown for at least three generations to the late logarithmic phase (0·8 O.D. at 600 nm) (Aronovitch et al 1991).…”

“…As EGC alone and 100 mmol l −1 did not kill E. coli (Table 1), the bactericidal action of EGC in the presence of Cu 2+ must be due to a different mechanism than that of EGC alone. Aronovitch et al (1991) have reported that EN causes little killing of E. coli in the presence of Cu 2+ , but rapid exponential killing is induced by the subsequent addition of H 2 O 2 . The bactericidal action of EN has been thought to involve activation of H 2 O 2 by the EN-Cu 2+ complex.…”