Bactericidal and Fungicidal Activities of Calia secundiflora (Ort.) Yakovlev

Abstract

Calia secundiflora (Ortega) Yakovlev (Fabaceae) is considered a medicinal plant in Mexico but has scarcely been used because of the toxicity of its quinolizidine alkaloids. Several quinolizidine alkaloids have shown bactericidal, nematicidal, and fungicidal activities. The purpose of this study was to identify the alkaloids in the seeds and evaluate the activity of the organic extract on several phytopathogenic fungi and bacteria. An in vitro bioassay was conducted with species of the following phytopathogenic fungi: Alternaria solani, Fusarium oxysporum and Monilia fructicola; and of the following bacteria Pseudomonas sp., Xanthomonas campestris and Erwinia carotovora. Cytisine, lupinine, anagyrine, sparteine, N-methylcytisine, 5,6-dehydrolupanine, and lupanine were identified by liquid chromatography-mass spectrometry in the extract of seeds; the most abundant compound of the extract was cytisine. It was observed that the crude extract of Calia secundiflora was moderately active on bacteria and more potent on phytopathogenic fungi. In contrast cytisine showed the opposite effects.

How do soil micro‐organisms respond to N, P and NP additions? Application of the ecological framework of (co‐)limitation by multiple resources

“…Furthermore, three archaeal taxa were negatively influenced by the plant Thermopsis lanceolata that did not share the same response type to nutrient addition; and Elymus nutans positively and negatively influenced 4 and 1 bacterial taxa, respectively, covering a broad range of response types. Interestingly, T. lanceolata is characterized by specific compounds like anagyrine, cytisine and thermopsine (Gao, Li, Jiang, & Zhu, ; Vinogradova, Iskandarov, & Yunusov, ) which have negative impacts on some bacteria (Pérezlaínez et al, ; Nemesio et al, ); A. rivularis roots are characterized by antioxidant compounds with antibacterial activity (Hao, Gu, & Xiao, ; Shi, Wei, Wei, & Wu, ) and E. nutans has been reported to exudate specific compounds that inhibit seed germination of several plants (Zeng, Mallik, & Luo, ). It is thus likely that the influence of these three plant species on particular microbial taxa is due to the effect of the very specific compounds they produce.…”

Quinolizidine-Based Variations and Antifungal Activity of Eight Lupinus Species Grown under Greenhouse Conditions

“…Finally, the activity of QREs having good contents of 3 and 10 (4.61–6.08 mg/g fl) was found to be moderate to low. In this regard, other QAs containing the 2-pyridone moiety (e.g., cytisine) have exhibited bactericidal and antifungal activities [ 54 , 57 ]. However, the antifungal activity of 3 and 10 could not be statistically recognized, possibly by low inhibition by structural reasons that should be deepened in further structure-activity relationship studies.…”

Extractos vegetales para el control de Colletotrichum gloeosporioides in vitro y en periodo de floración y poscosecha del fruto de Carica papaya

“…Principalmente, los alcaloides del grupo quinolizidínicos y tropánicos han sido los más estudiados por su actividad fungicida (Wink, 1992(Wink, , 1993. Pérez-Laínez et al (2008) reportaron la actividad fungicida de alcaloides quinolizidínicos presentes en Calia secundiflora contra Alternaria solani, Fusarium oxysporum y Monilia fructicola. Usha et al (2009) señalaron la actividad de D. stramonium contra Fusarium mangiferae debido a los alcaloides tropánicos presentes en diversas partes de la planta; así como, Haiyan et al (2005) reportaron su actividad contra Alternaria sp.…”

“…En M. parviflora se ha señalado la presencia de alcaloides y la de otros metaboliltos (compuestos fenólicos y flavonoides) (Farhan et al, 2012). Pérez-Laínez et al (2008) señalaron que los alcaloides afectan varios sitios a nivel molecular (inhibición del ADN y ARN, inhibición de la biosíntesis de proteínas y alteración de la estabilidad de membranas en microorganismos y células animales y la inhibición del crecimiento celular) (Brahmachari et al, 2013). En el tercer grupo se ubicó únicamente al extracto de la especie P. icosandra, en este extracto se identificó la presencia de terpenoides.…”