Bactericidal and virucidal activity of the alkalophilic P395D/L241V/T343A mutant of vanadium chloroperoxidase

Abstract

For many disinfection formulations, antimicrobial activity at slightly alkaline pH values is required. To date, only the wild-type vanadium chloroperoxidase has been studied for its antibacterial activity, and only at acidic to neutral pH values. Its antiviral activity (e.g. useful for the cleaning of medical equipment) was not studied before. The observed activity for the alkalophilic P395D/L241V/T343A mutant is an important step forward in the application of this robust enzyme as a component in disinfection formulations.

Possible role of reactive chlorine in microbial antagonism and organic matter chlorination in terrestrial environments

“…chloride, hydrogen peroxide and the activity of chlorinating enzymes, must be ‘sufficient’. Dose–response effects of microbially produced reactive chlorine on microorganisms have to our knowledge been investigated only for the haloperoxidase system of Curvularia sp., moulds belonging to the ascomycetes (Hansen et al ., 2003; 2004; 2005; Klebanoff, 2005; Pattison and Davies, 2006; Yap et al ., 2007; Renirie et al ., 2008). The studies show that the Curvularia system ‘has a rapid antimicrobial effect against a broad spectrum of bacteria, yeasts and filamentous fungi’ (Hansen et al ., 2003).…”

BioNano engineered hybrids for hypochlorous acid generation

“…We hypothesized that carboxyl functionalized multi-walled carbon nanotubes (MWNTs-COOH) can be used to allow generation of H 2 O 2 substrate necessary for the conversion of (Cl − ) into HOCl in the presence of CPO. HOCl has a wide spectrum of activity against a variety of pathogens from spores to bacteria [30,31,36]. Our hypothesis was that H 2 O 2 substrate can be produced through an enzymatic chain reaction at the MWNT interface.…”

“…HOCl has a wide spectrum of activity against a variety of pathogens such as spores and bacteria [30,31,36]. Specifically, we are employing a strategy to allow the working enzyme chloroperoxidase (CPO) to use H 2 O 2 produced at a nanosupport interface to convert (Cl − ) into HOCl.…”

Antimicrobial Activity of Vanadium Chloroperoxidase on Planktonic <i>Streptococcus mutans</i> Cells and <i>Streptococcus mutans</i> Biofilms

“…In our study the VCPO of C. inaequalis [Messerschmidt and Wever, 1996] was used to produce hypohalous acid from H 2 O 2 and the halides chloride and bromide. Previous work performed by Renirie et al [2008] showed that VCPO, at pH 8.0, in combination with 0.1 M Br -and 0.01 M H 2 O 2 , resulted in a 1-2 log 10 reduction in the viability of planktonic Staphylococcus aureus and Pseudomonas aeruginosa cells.…”

“…The antimicrobial activity of vanadium chloroperoxidase (VCPO) from Curvularia inaequalis has previously been investigated; the enzyme was found to be effective against planktonic bacteria, yeasts, fungi and viruses [Hansen et al, 2003;Renirie et al, 2008], but it has never been tested before on oral bacteria growing in either planktonic phase or biofilms. VCPO is able to catalyze the oxidation of halides (chloride, bromide and iodide) and has been shown to have enzymatic activity in a broad pH range between pH 4.0 and 8.0 with a pH optimum of around 5.5 [Van Schijndel et al, 1993].…”