Bactericidal effects of propolis/polylactic acid (PLA) nanofibres obtained via electrospinning

Abstract

Electrospinning of propolis / polylactic acid (PLA) fibres was investigated for propolis concentrations from 0-10% (w/v). Solutions could not be spun when the amount of propolis was over 8% (w/v). Mechanical characterization indicated that propolis reduced the tensile strength. The fibre mats exhibited poorer mechanical performance when the propolis concentration was increased. At propolis concentrations of 2-6% (w/v), the fibres were smooth, bead-free and homogeneous. When the concentration of propolis was more than 6% (w/v), the fibres changed from ductile to brittle. The fibres containing 2% (w/v) propolis showed bactericidal effects on Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis and Escherichia coli. At a propolis concentration of 4% (w/v) they also inhibited Bacillus cereus. Efectos bactericidas de nanofibras de propóleos/ácido poliláctico (APL) obtenidas mediante electrohilado (electrospinning) ResumenEl electrohilado de fibras de propóleos/ácido poliláctico (APL) se ha investigado con concentraciones de propóleos de 0-10% (w/v). Las disoluciones no pudieron ser hiladas cuando la cantidad de propóleos fue superior al 8% (w/v). La caracterización mecánica indicó que el propóleos reduce la resistencia a la tracción. Las esteras de fibra tuvieron un comportamiento mecánico más pobre cuando se aumentó la concentración de propóleos. A concentraciones de propóleos de 2-6% (w/v), las fibras eran lisas, sin bolitas y homogéneas. Cuando la concentración de propóleos fue de más de 6% (w/v), las fibras cambiaron de dúctil a frágil. Las fibras con 2% (w/v) de propóleos mostraron efectos bactericidas sobre Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis y Escherichia coli. A una concentración de propóleos de 4% (w/v) también inhibieron Bacillus cereus.

Development and Evaluation of Biomimetic 3D Coated Composite Scaffold for Application as Skin Substitutes

“…[ 31,32 ] Among the biocompatible scaffold materials, polylactic acid (PLA) and PCL were widely used in tissue engineering through electrospinning or EHD jetting techniques because of their good mechanical and structural properties. [ 30,33,34 ] However, these materials present low bioactivity and hydrophobicity, which could reduce cell affinity and low tissue regeneration rates. [ 35 ] Therefore, the surface modification of electrospinning and EHD jetting scaffold has been studied by combining other biomaterials to take advantages of individual components for wound healing.…”

Propolis Extract-PVA Nanocomposites of Textile Design: Antimicrobial Effect on Gram Positive and Negative Bacterias

“…Electrospinnability of propolis by help of polymer solutions has been investigated with other types of polymers in recent studies [13,14].…”

Dichloromethane-Extract of Propolis (DEP) and DEP/PLA Electrospun Fiber Membranes

“…Propolis nanofiber fiber membrane with antibacterial activity were prepared by an electrospinning technique for different application areas. Propolis extract was used as an active ingredient, and polyvinylpyrrolidone (PVP) K90, polycaprolactone (PCL), polyurethane (PU) , polyvinyl alcohol (PVA) and Polycaprolactone (PCL) were used as the polymer matrix [24][25][26][27][28][29]. The antibacterial activity could be used against Streptococcus mutans, Staphylococcus aureus (S. aureus), Staphylococcus epidermidis, Proteus mirabilis, Bacillus cereus, and Escherichia coli, as well as against gram positive bacteria (S. aureus), gram negative bacteria (A. baumannii and P. aeruginosa) [31].…”

“…Previous mechanical characterisation showed that propolis reduces the tensile strength. Fiber mats exhibit worse mechanical performance when the propolis concentration increases [27]. Propolis has an effect on the mechanical properties of the fiber mat, hence fiber mat mechanical properties are improved by adding nanocellulose and polyurethane PU [25][26].…”