Bactericidal synergy of lysostaphin in combination with antimicrobial peptides

Desbois, Andrew P.; Coote, Peter J.

doi:10.1007/s10096-011-1188-z

Cited by 25 publications

(19 citation statements)

References 35 publications

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“…To date, several successful trials of lysostaphin containing combination treatments have been described. The enzyme shown to be synergistically bactericidal in combination with various agents, including antibiotics (Polak et al 1993; Climo et al 1998; Kiri et al 2002; Walencka et al 2006), antimicrobial peptides (Desbois and Coote 2011), and even some natural products, e.g., tea tree oil (LaPlante 2007). The similar effects have been observed in the case of several other PHs, e.g., ClyS, which showed synergistic interactions with vancomycin and oxacillin.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Desbois and Coote (2011) revealed a strong bactericidal synergy of lysostaphin in combination with some other antimicrobial peptides. It is also highly possible that the bactericidal effect of the enzyme can be enhanced by some food preservatives or natural substances (so far not tested).…”

Section: Peptidoglycan Hydrolases With Antistaphylococcal Activitymentioning

confidence: 99%

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Peptidoglycan hydrolases-potential weapons against Staphylococcus aureus

Szweda

Schielmann

Kotłowski

et al. 2012

Appl Microbiol Biotechnol

105

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Bacteria of the genus Staphylococcus are common pathogens responsible for a broad spectrum of human and animal infections and belong to the most important etiological factors causing food poisoning. Because of rapid increase in the prevalence of isolation of staphylococci resistant to many antibiotics, there is an urgent need for the development of new alternative chemotherapeutics. A number of studies have recently demonstrated the strong potential of peptidoglycan hydrolases (PHs) to control and treat infections caused by this group of bacteria. PHs cause rapid lysis and death of bacterial cells. The review concentrates on enzymes hydrolyzing peptidoglycan of staphylococci. Usually, they are characterized by high specificity to only Staphylococcus aureus cell wall components; however, some of them are also able to lyse cells of other staphylococci, e.g., Staphylococcus epidermidis-human pathogen of growing importance and also other groups of bacteria. Some PHs strengthen the bactericidal or bacteriostatic activity of common antibiotics, and as a result, they should be considered as component of combined therapy which could definitely reduced the development of bacterial resistance to both enzymes and antibiotics. The preliminary research revealed that most of these enzymes can be produced using heterologous, especially Escherichia coli expression systems; however, still much effort is required to develop more efficient and large-scale production technologies. This review discusses current state on knowledge with emphasis on the possibilities of application of PHs in the context of therapeutics for infections caused by staphylococci.

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Section: Discussionmentioning

confidence: 99%

Section: Peptidoglycan Hydrolases With Antistaphylococcal Activitymentioning

confidence: 99%

Peptidoglycan hydrolases-potential weapons against Staphylococcus aureus

Szweda

Schielmann

Kotłowski

et al. 2012

Appl Microbiol Biotechnol

105

View full text Add to dashboard Cite

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“…Less information exists about the microbicidal action of Lf against fungi and protozoa [59,60,61,62]. Very important is the finding that Lf synergizes with antibiotics and drugs, and even with other proteins of the innate immune system such as lysozyme and natural secretory IgA (sIgA) antibodies, potentiating the antimicrobial effect [63,64,65,66,67,68,69]. …”

Section: Lactoferrin: Distribution Structure and Biological Functmentioning

confidence: 99%

Lactoferrin: Balancing Ups and Downs of Inflammation Due to Microbial Infections

Drago-Serrano

Campos-Rodrı́guez

Carrero

et al. 2017

IJMS

115

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Lactoferrin (Lf) is a glycoprotein of the primary innate immune-defense system of mammals present in milk and other mucosal secretions. This protein of the transferrin family has broad antimicrobial properties by depriving pathogens from iron, or disrupting their plasma membranes through its highly cationic charge. Noteworthy, Lf also exhibits immunomodulatory activities performing up- and down-regulation of innate and adaptive immune cells, contributing to the homeostasis in mucosal surfaces exposed to myriad of microbial agents, such as the gastrointestinal and respiratory tracts. Although the inflammatory process is essential for the control of invasive infectious agents, the development of an exacerbated or chronic inflammation results in tissue damage with life-threatening consequences. In this review, we highlight recent findings in in vitro and in vivo models of the gut, lung, oral cavity, mammary gland, and liver infections that provide experimental evidence supporting the therapeutic role of human and bovine Lf in promoting some parameters of inflammation and protecting against the deleterious effects of bacterial, viral, fungal and protozoan-associated inflammation. Thus, this new knowledge of Lf immunomodulation paves the way to more effective design of treatments that include native or synthetic Lf derivatives, which may be useful to reduce immune-mediated tissue damage in infectious diseases.

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“…The effects of AMPs combined with antibiotics often exceed those of the individual drugs, whether in antibacterial activities5–8 or in interruption of biofilm formation 9–13. Drug design research faces a long and difficult challenge to overcome drug resistance, whereas drug combinations require no drug modification and lower doses can be used while enhancing the therapeutic potential 14,15…”

Section: Introductionmentioning

confidence: 99%

Synergistic effects of antimicrobial peptide DP7 combined with antibiotics against multidrug-resistant bacteria

Wu¹,

Li²,

Li³

et al. 2017

DDDT

120

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Antibiotic-resistant bacteria present a great threat to public health. In this study, the synergistic effects of antimicrobial peptides (AMPs) and antibiotics on several multidrug-resistant bacterial strains were studied, and their synergistic effects on azithromycin (AZT)-resistance genes were analyzed to determine the relationships between antimicrobial resistance and these synergistic effects. A checkerboard method was used to evaluate the synergistic effects of AMPs (DP7 and CLS001) and several antibiotics (gentamicin, vancomycin [VAN], AZT, and amoxicillin) on clinical bacterial strains (Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, and Escherichia coli). The AZT-resistance genes (ermA, ermB, ermC, mefA, and msrA) were identified in the resistant strains using quantitative polymerase chain reaction. For all the clinical isolates tested that were resistant to different antibiotics, DP7 had high antimicrobial activity (≤32 mg/L). When DP7 was combined with VAN or AZT, the effect was most frequently synergistic. When we studied the resistance genes of the AZT-resistant isolates, the synergistic effect of DP7–AZT occurred most frequently in highly resistant strains or strains carrying more than two AZT-resistance genes. A transmission electron microscopic analysis of the S. aureus strain synergistically affected by DP7–AZT showed no noteworthy morphological changes, suggesting that a molecular-level mechanism plays an important role in the synergistic action of DP7–AZT. AMP DP7 plus the antibiotic AZT or VAN is more effective, especially against highly antibiotic-resistant strains.

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Bactericidal synergy of lysostaphin in combination with antimicrobial peptides

Cited by 25 publications

References 35 publications

Peptidoglycan hydrolases-potential weapons against Staphylococcus aureus

Peptidoglycan hydrolases-potential weapons against Staphylococcus aureus

Lactoferrin: Balancing Ups and Downs of Inflammation Due to Microbial Infections

Synergistic effects of antimicrobial peptide DP7 combined with antibiotics against multidrug-resistant bacteria

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