Bacteriocin (Marcescin) Typing of Clinical Isolates of Serratia marcescens

Traub, Walter H.; Raymond, E. A.; Startsman, T. S.

doi:10.1128/aem.21.5.837-840.1971

Cited by 44 publications

(24 citation statements)

References 4 publications

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“…Biotyping provides a current way for discriminating between nosocomial strains, but only biotypes A5, A8a, A8b, and TCT are predominantly represented in hospital wards (13). Other methods, including serotyping (26), phage typing (9), testing for bacteriocin production or susceptibility (7,8,27,28), and electrophoresis of outer membrane proteins (2) or total proteins (15,20), have been found to be unsatisfactory because of insufficient discrimination and/or poor reproducibility. Restriction endonuclease analysis of DNA with conventional agarose gel electrophoresis has been successfully used for epidemiological fingerprinting.…”

Section: Discussionmentioning

confidence: 99%

Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping

et al. 1995

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Ribotyping carried out with a nonradioactive probe (acetylaminofluorene ribosomal RNA kit I from Eurogentec, Seraing, Belgium) was performed for the characterization of 139 hospital strains of Serratia marcescens. These strains, which belonged to 11 biotypes and 1 nontypeable group, were isolated in seven hospitals in Belgium between 1986 and 1992. EcoRI and HindIII were used to obtain cleavage patterns. Analysis of the results produced 27 different patterns with EcoRI and 23 patterns with HindIII. Typeability reached 100%. Combination of the patterns obtained with each enzyme produced 38 distinct ribotypes. Percent similarity values, calculated by using the Dice coefficient and unweighted-pair group average linkage clustering, showed four main clusters and nine subclusters of ribopatterns at a similarity rate of approximately 80% or less. These groups did not coincide with those delimited by biotyping, although a rather good correlation was observed. The simultaneous use of the two methods has potential value in epidemiological studies. and ESN, five other hospitals in the Liège area; BXL, Hôpital Universitaire Brugmann, Brussels, Belgium. b ?, month unknown. c RFLP, restriction fragment length morphism.2638 CHETOUI ET AL. J. CLIN. MICROBIOL.

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Section: Discussionmentioning

confidence: 99%

Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping

et al. 1995

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“…From 1 June 1970, through 15 April 1971, 155 isolates of S. marcescenis were cultured from 105 patients. The isolates were identified as previously described (19), as were 24 isolates that had been encountered a year earlier, frozen, and stored at -65 C (20). The isolates were typed by bacteriocin sensitivity as previously reported (20).…”

Section: Methodsmentioning

confidence: 99%

Epidemiological Surveillance of Serratia marcescens Infections by Bacteriocin Typing

Traub¹,

Raymond²

1971

Applied Microbiology

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During 10 months, 155 isolates of Serratia marcescens were cultured from 105 patients, of whom 49 were considered to have significant infection. The 155 isolates were typed by bacteriocin sensitivity, and 137 (88.4%) were assigned to 37 provisional bacteriocin groups; 18 isolates were nontypable. No major outbreaks of nosocomial infection were demonstrable; however, there were four chronologically separate minor episodes of cross-infection that involved two or tbree patients per room or unit, respectively.

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“…Figure 1 shows clear zones due to bacteriocins on one of the indicators. Zones 1,2,3,7,8,11,15,16,22,and 23 are completely clear, but zone 13 has about 50 colonies growing in it. The remaining zones are identical to control 24, and are thus defined as negative.…”

Section: Resultsmentioning

confidence: 99%

“…Ewing, Davis, and Reavis (4, 5) reported a method for determining 15 0 antigens and 13 H antigens, and serological differentiation based on this scheme has been used in epidemiology (5,15,20,24). Differentiation based on bacteriophage susceptibility (16,23) and bacteriocin production or sensitivity (11,12,17, 22) has been described, but only the recent method of Traub, Raymond, and Startsman (22) was designed for routine typing. Farmer and Herman (8) recently developed a sensitive typing method for P. aeruginosa by testing a large number of bacteriocin indicator strains and choosing only those most useful for differentiation.…”

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confidence: 99%

Epidemiological Differentiation of Serratia marcescens: Typing by Bacteriocin Production1

Farmer¹

1972

Applied Microbiology

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A new method for comparing and differentiating strains of S. marcescens is described which has proved useful in determining the epidemiology of hospital infections. Strains were grown in Trypticase soy broth, and bacteriocin production was induced with mitomycin C for 5 hr. The bacteriocin lysates were then spotted onto nine standard indicator strains, which were chosen with the aid of computer analysis from the 118 indicators tested. After 24 hr at 37 C, zones of inhibition due to bacteriocins were recorded. One hundred twentynine strains were differentiated into 72 different bacteriocin production patterns, but 11 strains were nontypable. None of the 45 other strains of Enterobacteriaceae produced bacteriocins. Bacteriocin production was a stable epidemiological marker. Colonial mutants always had identical patterns, as did the same strain which has passed from patient to patient through cross-infection. The new technique does not require any specialized equipment and can be used in laboratories with limited budgets. The applications of the new method in cross-infection studies and as a supplement to serological typing are discussed.

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Bacteriocin (Marcescin) Typing of Clinical Isolates of Serratia marcescens

Cited by 44 publications

References 4 publications

Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping

Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping

Epidemiological Surveillance of Serratia marcescens Infections by Bacteriocin Typing

Epidemiological Differentiation of Serratia marcescens: Typing by Bacteriocin Production1

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